Profiling of the bacterial community and the degradative capability of newly isolated poly(lactic acid) (PLA)- and poly(butylene succinate) (PBS)-degrading bacteria from coastal samples.
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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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3 Biotech
2025
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| _version_ | 1868266149512216577 |
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| author | Phonlamai, Ausawadee Khetkorn, Wanthanee Thongpool, Voranuch Panyachanakul, Titiporn Suriyachadkun, Chanwit Kitpreechavanich, Vichien Sakdapetsiri, Chatsuda Lomthong, Thanasak |
| author_facet | Phonlamai, Ausawadee Khetkorn, Wanthanee Thongpool, Voranuch Panyachanakul, Titiporn Suriyachadkun, Chanwit Kitpreechavanich, Vichien Sakdapetsiri, Chatsuda Lomthong, Thanasak Phonlamai, Ausawadee Khetkorn, Wanthanee Thongpool, Voranuch Panyachanakul, Titiporn Suriyachadkun, Chanwit Kitpreechavanich, Vichien Sakdapetsiri, Chatsuda Lomthong, Thanasak |
| collection | PubMed - marine biology |
| contents | Profiling of the bacterial community and the degradative capability of newly isolated poly(lactic acid) (PLA)- and poly(butylene succinate) (PBS)-degrading bacteria from coastal samples. Phonlamai, Ausawadee Khetkorn, Wanthanee Thongpool, Voranuch Panyachanakul, Titiporn Suriyachadkun, Chanwit Kitpreechavanich, Vichien Sakdapetsiri, Chatsuda Lomthong, Thanasak The coastal area of Thailand is a tropical marine environment with high microbial diversity, providing favorable conditions for microorganisms capable of degrading bioplastics. The current study aimed to investigate the bacterial community profiling of four samples collected from a coastal area in Thailand and to isolate the potential thermophilic bacteria with the ability to produce bioplastic-degrading enzymes. Our analysis revealed site-specific predominant genera: in seawater (64.34 ± 0.27%), in plastic waste (39.69 ± 3.77%), in soil (54.83 ± 2.40%), and in moss rock (41.01 ± 1.67%). The thermophilic bacteria, including 6 poly(lactic acid) (PLA)- and 3 poly(butylene succinate) (PBS)-degrading bacteria, were isolated using a two-step technique in an emulsified polymer medium. These nine isolates were classified into five species across four genera: , , , , and . Among these, LDF1 and BS2-4 exhibited the highest enzymatic activities for PLA and PBS degradation (0.87 ± 0.11 U/mL and 0.31 ± 0.03 U/mL, respectively). Scanning electron microscopy confirmed the degradation capabilities of these strains in culture medium. Crude enzyme from the LDF1 strain demonstrated versatility in degrading various types of PLA, including PLA film, PLA powder, commercial cup, and commercial cutlery, while the strain BS2-4 enzyme effectively degraded PBS in film, powder, commercial cup, and commercial drinking straw. These findings advance our understanding of coastal microbial ecology and also highlight the potential of indigenous bacteria for bioplastic waste management, contributing to sustainable environmental solutions. The online version contains supplementary material available at 10.1007/s13205-025-04521-0. |
| format | Artículo científico |
| id | pubmed_40978322 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | 3 Biotech |
| record_format | pubmed |
| spellingShingle | Profiling of the bacterial community and the degradative capability of newly isolated poly(lactic acid) (PLA)- and poly(butylene succinate) (PBS)-degrading bacteria from coastal samples. Phonlamai, Ausawadee Khetkorn, Wanthanee Thongpool, Voranuch Panyachanakul, Titiporn Suriyachadkun, Chanwit Kitpreechavanich, Vichien Sakdapetsiri, Chatsuda Lomthong, Thanasak Profiling of the bacterial community and the degradative capability of newly isolated poly(lactic acid) (PLA)- and poly(butylene succinate) (PBS)-degrading bacteria from coastal samples. Phonlamai, Ausawadee Khetkorn, Wanthanee Thongpool, Voranuch Panyachanakul, Titiporn Suriyachadkun, Chanwit Kitpreechavanich, Vichien Sakdapetsiri, Chatsuda Lomthong, Thanasak The coastal area of Thailand is a tropical marine environment with high microbial diversity, providing favorable conditions for microorganisms capable of degrading bioplastics. The current study aimed to investigate the bacterial community profiling of four samples collected from a coastal area in Thailand and to isolate the potential thermophilic bacteria with the ability to produce bioplastic-degrading enzymes. Our analysis revealed site-specific predominant genera: in seawater (64.34 ± 0.27%), in plastic waste (39.69 ± 3.77%), in soil (54.83 ± 2.40%), and in moss rock (41.01 ± 1.67%). The thermophilic bacteria, including 6 poly(lactic acid) (PLA)- and 3 poly(butylene succinate) (PBS)-degrading bacteria, were isolated using a two-step technique in an emulsified polymer medium. These nine isolates were classified into five species across four genera: , , , , and . Among these, LDF1 and BS2-4 exhibited the highest enzymatic activities for PLA and PBS degradation (0.87 ± 0.11 U/mL and 0.31 ± 0.03 U/mL, respectively). Scanning electron microscopy confirmed the degradation capabilities of these strains in culture medium. Crude enzyme from the LDF1 strain demonstrated versatility in degrading various types of PLA, including PLA film, PLA powder, commercial cup, and commercial cutlery, while the strain BS2-4 enzyme effectively degraded PBS in film, powder, commercial cup, and commercial drinking straw. These findings advance our understanding of coastal microbial ecology and also highlight the potential of indigenous bacteria for bioplastic waste management, contributing to sustainable environmental solutions. The online version contains supplementary material available at 10.1007/s13205-025-04521-0. |
| title | Profiling of the bacterial community and the degradative capability of newly isolated poly(lactic acid) (PLA)- and poly(butylene succinate) (PBS)-degrading bacteria from coastal samples. |
| url | https://pubmed.ncbi.nlm.nih.gov/40978322/ |