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Main Authors: Hirakawa, Yuta, Koga, Toshiki, Suzuki, Takahiro, Ohkouchi, Naohiko, Takano, Yoshinori
Format: Artículo científico
Language:en
Published: ACS omega 2025
Online Access:https://pubmed.ncbi.nlm.nih.gov/40978342/
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author Hirakawa, Yuta
Koga, Toshiki
Suzuki, Takahiro
Ohkouchi, Naohiko
Takano, Yoshinori
author_facet Hirakawa, Yuta
Koga, Toshiki
Suzuki, Takahiro
Ohkouchi, Naohiko
Takano, Yoshinori
Hirakawa, Yuta
Koga, Toshiki
Suzuki, Takahiro
Ohkouchi, Naohiko
Takano, Yoshinori
collection PubMed - marine biology
contents Analytical Development for Detecting Femtomole-Level Organophosphorus Compounds in Biogeochemical Samples by Ion Chromatography/High-Resolution Mass Spectrometry. Hirakawa, Yuta Koga, Toshiki Suzuki, Takahiro Ohkouchi, Naohiko Takano, Yoshinori Phosphorus (P) is a crucial element for life, and its organic forms play a central role in modern biology. Therefore, the analysis of their presence in environmental samples is important for evaluating the biogeochemistry of P and the P cycle on the Earth. Herein, we developed a high-sensitivity, high-resolution analytical technique for detecting organophosphorus compounds using ion chromatography (IC) with Orbitrap-mass spectrometry (Orbitrap-MS). The IC method clearly separated organophosphorus compounds, including alkyl phosphonates and alkyl phosphates, from other organic molecules and inorganic ions. The online combination of IC and Orbitrap-MS enabled the quantification of organophosphorus compounds with concentrations as low as ∼10 fmol and their molecular identification in the presence of various interfering organic substances. The feasibility of the developed and optimized IC-Orbitrap-MS method was confirmed by analyzing deep-sea sediments obtained from 343 m below the seafloor. This developed analytical technique can also be applied to biogeochemical and astrochemical molecular surveys of organophosphorus compounds in natural environments.
format Artículo científico
id pubmed_40978342
institution PubMed
language en
publishDate 2025
publisher ACS omega
record_format pubmed
spellingShingle Analytical Development for Detecting Femtomole-Level Organophosphorus Compounds in Biogeochemical Samples by Ion Chromatography/High-Resolution Mass Spectrometry.
Hirakawa, Yuta
Koga, Toshiki
Suzuki, Takahiro
Ohkouchi, Naohiko
Takano, Yoshinori
Analytical Development for Detecting Femtomole-Level Organophosphorus Compounds in Biogeochemical Samples by Ion Chromatography/High-Resolution Mass Spectrometry. Hirakawa, Yuta Koga, Toshiki Suzuki, Takahiro Ohkouchi, Naohiko Takano, Yoshinori Phosphorus (P) is a crucial element for life, and its organic forms play a central role in modern biology. Therefore, the analysis of their presence in environmental samples is important for evaluating the biogeochemistry of P and the P cycle on the Earth. Herein, we developed a high-sensitivity, high-resolution analytical technique for detecting organophosphorus compounds using ion chromatography (IC) with Orbitrap-mass spectrometry (Orbitrap-MS). The IC method clearly separated organophosphorus compounds, including alkyl phosphonates and alkyl phosphates, from other organic molecules and inorganic ions. The online combination of IC and Orbitrap-MS enabled the quantification of organophosphorus compounds with concentrations as low as ∼10 fmol and their molecular identification in the presence of various interfering organic substances. The feasibility of the developed and optimized IC-Orbitrap-MS method was confirmed by analyzing deep-sea sediments obtained from 343 m below the seafloor. This developed analytical technique can also be applied to biogeochemical and astrochemical molecular surveys of organophosphorus compounds in natural environments.
title Analytical Development for Detecting Femtomole-Level Organophosphorus Compounds in Biogeochemical Samples by Ion Chromatography/High-Resolution Mass Spectrometry.
url https://pubmed.ncbi.nlm.nih.gov/40978342/