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| Format: | Artículo científico |
| Sprache: | en |
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Materials today. Bio
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/40989832/ |
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| author | Islam, Mohammad Shafiqul Noman, Muhammad Zhang, Zhen Ahmed, Temoor Cai, Yingying Wang, Jing Ali, Md Arshad Hafeez, Rahila Qiu, Haiping Hao, Zhongna Chai, Rongyao Ijaz, Munazza Wang, Yanli Li, Bin Wang, Jiaoyu |
| author_facet | Islam, Mohammad Shafiqul Noman, Muhammad Zhang, Zhen Ahmed, Temoor Cai, Yingying Wang, Jing Ali, Md Arshad Hafeez, Rahila Qiu, Haiping Hao, Zhongna Chai, Rongyao Ijaz, Munazza Wang, Yanli Li, Bin Wang, Jiaoyu Islam, Mohammad Shafiqul Noman, Muhammad Zhang, Zhen Ahmed, Temoor Cai, Yingying Wang, Jing Ali, Md Arshad Hafeez, Rahila Qiu, Haiping Hao, Zhongna Chai, Rongyao Ijaz, Munazza Wang, Yanli Li, Bin Wang, Jiaoyu |
| collection | PubMed - marine biology |
| contents | Advanced bifunctional nanotherapeutics display multi-tiered defense against through targeted fungal inhibition and rice immunity enhancement. Islam, Mohammad Shafiqul Noman, Muhammad Zhang, Zhen Ahmed, Temoor Cai, Yingying Wang, Jing Ali, Md Arshad Hafeez, Rahila Qiu, Haiping Hao, Zhongna Chai, Rongyao Ijaz, Munazza Wang, Yanli Li, Bin Wang, Jiaoyu Rice blast disease, caused by (), severely threatens global rice production. In this study, biogenic copper nanoparticles (bio-CuNPs) were synthesized extracellularly using Q1 and characterized for antifungal activity and plant defense induction. Bio-CuNPs (16-62 nm, average 37 nm) exhibited potent antifungal effects by significantly inhibiting mycelial growth, conidial germination, and appressorium formation in a dose-dependent manner. Microscopic observations revealed that bio-CuNPs disrupted hyphal integrity, caused intracellular leakage, and induced DNA damage. Transcriptomic profiling identified key regulatory genes in upon CuNP exposure, with key disruptions in cell wall biosynthesis (, , , and ) and membrane transport pathways (, and ). Deletion mutants for these genes demonstrated heightened sensitivity to bio-CuNPs, indicating that these genes are critical for helping to withstand the antifungal effects of bio-CuNPs. However, bio-CuNPs disrupted their functions in , confirming these genes as one of the molecular targets to suppress growth and virulence. assays revealed that bio-CuNP foliar application reduced disease severity, improved plant growth, and activated antioxidant enzymes, while suppressing oxidative stressors (, super oxide radicle and hygrogen peroxide). Metabolomic analysis revealed significant alterations in defense-related pathways, including phenylpropanoid and amino acid metabolism. Additionally, CuNPs enhanced salicylic acid and methyl jasmonate levels, which subsequently upregulated defense gene expression. Cytotoxicity assays revealed that bio-CuNPs were non-toxic to AML12 cells at effective concentrations, highlighting their potential as an eco-friendly strategy for sustainable rice blast management. |
| format | Artículo científico |
| id | pubmed_40989832 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Materials today. Bio |
| record_format | pubmed |
| spellingShingle | Advanced bifunctional nanotherapeutics display multi-tiered defense against through targeted fungal inhibition and rice immunity enhancement. Islam, Mohammad Shafiqul Noman, Muhammad Zhang, Zhen Ahmed, Temoor Cai, Yingying Wang, Jing Ali, Md Arshad Hafeez, Rahila Qiu, Haiping Hao, Zhongna Chai, Rongyao Ijaz, Munazza Wang, Yanli Li, Bin Wang, Jiaoyu Advanced bifunctional nanotherapeutics display multi-tiered defense against through targeted fungal inhibition and rice immunity enhancement. Islam, Mohammad Shafiqul Noman, Muhammad Zhang, Zhen Ahmed, Temoor Cai, Yingying Wang, Jing Ali, Md Arshad Hafeez, Rahila Qiu, Haiping Hao, Zhongna Chai, Rongyao Ijaz, Munazza Wang, Yanli Li, Bin Wang, Jiaoyu Rice blast disease, caused by (), severely threatens global rice production. In this study, biogenic copper nanoparticles (bio-CuNPs) were synthesized extracellularly using Q1 and characterized for antifungal activity and plant defense induction. Bio-CuNPs (16-62 nm, average 37 nm) exhibited potent antifungal effects by significantly inhibiting mycelial growth, conidial germination, and appressorium formation in a dose-dependent manner. Microscopic observations revealed that bio-CuNPs disrupted hyphal integrity, caused intracellular leakage, and induced DNA damage. Transcriptomic profiling identified key regulatory genes in upon CuNP exposure, with key disruptions in cell wall biosynthesis (, , , and ) and membrane transport pathways (, and ). Deletion mutants for these genes demonstrated heightened sensitivity to bio-CuNPs, indicating that these genes are critical for helping to withstand the antifungal effects of bio-CuNPs. However, bio-CuNPs disrupted their functions in , confirming these genes as one of the molecular targets to suppress growth and virulence. assays revealed that bio-CuNP foliar application reduced disease severity, improved plant growth, and activated antioxidant enzymes, while suppressing oxidative stressors (, super oxide radicle and hygrogen peroxide). Metabolomic analysis revealed significant alterations in defense-related pathways, including phenylpropanoid and amino acid metabolism. Additionally, CuNPs enhanced salicylic acid and methyl jasmonate levels, which subsequently upregulated defense gene expression. Cytotoxicity assays revealed that bio-CuNPs were non-toxic to AML12 cells at effective concentrations, highlighting their potential as an eco-friendly strategy for sustainable rice blast management. |
| title | Advanced bifunctional nanotherapeutics display multi-tiered defense against through targeted fungal inhibition and rice immunity enhancement. |
| url | https://pubmed.ncbi.nlm.nih.gov/40989832/ |