Salvato in:
| Autori principali: | , , , , , , , |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
ISME communications
2025
|
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/40994829/ |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1868266149481807872 |
|---|---|
| author | Sultana, Sabiha Bruns, Stefan Pacheco-Valenciana, Armando Mehrshad, Maliheh Wilkes, Heinz Simon, Meinhard Garcia, Sarahi Wienhausen, Gerrit |
| author_facet | Sultana, Sabiha Bruns, Stefan Pacheco-Valenciana, Armando Mehrshad, Maliheh Wilkes, Heinz Simon, Meinhard Garcia, Sarahi Wienhausen, Gerrit Sultana, Sabiha Bruns, Stefan Pacheco-Valenciana, Armando Mehrshad, Maliheh Wilkes, Heinz Simon, Meinhard Garcia, Sarahi Wienhausen, Gerrit |
| collection | PubMed - marine biology |
| contents | Vitamin B release through bacteriophage-mediated cell lysis of the marine bacterium sp. M39. Sultana, Sabiha Bruns, Stefan Pacheco-Valenciana, Armando Mehrshad, Maliheh Wilkes, Heinz Simon, Meinhard Garcia, Sarahi Wienhausen, Gerrit Vitamin B (B) is an essential cofactor for vital metabolic processes in both prokaryotes and eukaryotes. B biosynthesis is exclusively carried out by a modicum of prokaryotes, although being required by most organisms. Recently, it has been demonstrated that not all B-prototrophic bacteria voluntarily share this vital cofactor and, therefore, are termed B-retainers. Consequently, low biosynthesis potential and limited voluntary release lead to a large discrepancy between availability and demand for B in the ocean, indicating that release of B may be an important control. Hence, in this study, we examined a specific release process, cell lysis after phage infection. We isolated bacteriophages specific for the B-prototrophic, yet B-retainer bacterium sp. M39. The addition of the bacteriophages to a sp. M39 mono-culture led to a significant increase in virus-like particles, reduced bacterial growth, and quantifiable extracellular dissolved B. When introducing bacteriophages to a co-culture comprising the host bacterium and the B-auxotrophic diatom , we observed rapid response in the form of microalgal growth. Our results indicate that B is released as a result of bacteriophage-mediated cell lysis of sp. M39, enabling the growth of in co-culture and possibly other microbes in nature. Therefore, we propose that bacteriophage-mediated cell lysis is a key mechanism for the release of essential metabolites, including vitamins, and given the estimated bacteriophage infection rates in the ocean, it plays a crucial role in the B-vitamin cycle in the marine environment. |
| format | Artículo científico |
| id | pubmed_40994829 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | ISME communications |
| record_format | pubmed |
| spellingShingle | Vitamin B release through bacteriophage-mediated cell lysis of the marine bacterium sp. M39. Sultana, Sabiha Bruns, Stefan Pacheco-Valenciana, Armando Mehrshad, Maliheh Wilkes, Heinz Simon, Meinhard Garcia, Sarahi Wienhausen, Gerrit Vitamin B release through bacteriophage-mediated cell lysis of the marine bacterium sp. M39. Sultana, Sabiha Bruns, Stefan Pacheco-Valenciana, Armando Mehrshad, Maliheh Wilkes, Heinz Simon, Meinhard Garcia, Sarahi Wienhausen, Gerrit Vitamin B (B) is an essential cofactor for vital metabolic processes in both prokaryotes and eukaryotes. B biosynthesis is exclusively carried out by a modicum of prokaryotes, although being required by most organisms. Recently, it has been demonstrated that not all B-prototrophic bacteria voluntarily share this vital cofactor and, therefore, are termed B-retainers. Consequently, low biosynthesis potential and limited voluntary release lead to a large discrepancy between availability and demand for B in the ocean, indicating that release of B may be an important control. Hence, in this study, we examined a specific release process, cell lysis after phage infection. We isolated bacteriophages specific for the B-prototrophic, yet B-retainer bacterium sp. M39. The addition of the bacteriophages to a sp. M39 mono-culture led to a significant increase in virus-like particles, reduced bacterial growth, and quantifiable extracellular dissolved B. When introducing bacteriophages to a co-culture comprising the host bacterium and the B-auxotrophic diatom , we observed rapid response in the form of microalgal growth. Our results indicate that B is released as a result of bacteriophage-mediated cell lysis of sp. M39, enabling the growth of in co-culture and possibly other microbes in nature. Therefore, we propose that bacteriophage-mediated cell lysis is a key mechanism for the release of essential metabolites, including vitamins, and given the estimated bacteriophage infection rates in the ocean, it plays a crucial role in the B-vitamin cycle in the marine environment. |
| title | Vitamin B release through bacteriophage-mediated cell lysis of the marine bacterium sp. M39. |
| url | https://pubmed.ncbi.nlm.nih.gov/40994829/ |