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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
ISME communications
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/40994829/ |
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Table of Contents:
- Vitamin B release through bacteriophage-mediated cell lysis of the marine bacterium sp. M39. Sultana, Sabiha Bruns, Stefan Pacheco-Valenciana, Armando Mehrshad, Maliheh Wilkes, Heinz Simon, Meinhard Garcia, Sarahi Wienhausen, Gerrit Vitamin B (B) is an essential cofactor for vital metabolic processes in both prokaryotes and eukaryotes. B biosynthesis is exclusively carried out by a modicum of prokaryotes, although being required by most organisms. Recently, it has been demonstrated that not all B-prototrophic bacteria voluntarily share this vital cofactor and, therefore, are termed B-retainers. Consequently, low biosynthesis potential and limited voluntary release lead to a large discrepancy between availability and demand for B in the ocean, indicating that release of B may be an important control. Hence, in this study, we examined a specific release process, cell lysis after phage infection. We isolated bacteriophages specific for the B-prototrophic, yet B-retainer bacterium sp. M39. The addition of the bacteriophages to a sp. M39 mono-culture led to a significant increase in virus-like particles, reduced bacterial growth, and quantifiable extracellular dissolved B. When introducing bacteriophages to a co-culture comprising the host bacterium and the B-auxotrophic diatom , we observed rapid response in the form of microalgal growth. Our results indicate that B is released as a result of bacteriophage-mediated cell lysis of sp. M39, enabling the growth of in co-culture and possibly other microbes in nature. Therefore, we propose that bacteriophage-mediated cell lysis is a key mechanism for the release of essential metabolites, including vitamins, and given the estimated bacteriophage infection rates in the ocean, it plays a crucial role in the B-vitamin cycle in the marine environment.