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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Biology
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41007270/ |
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| _version_ | 1868266149456642049 |
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| author | Zeng, Zishan Lin, Nanxin Lu, Tao Xu, Jian Zhang, Zheng Wang, Fang Wang, Jinzi |
| author_facet | Zeng, Zishan Lin, Nanxin Lu, Tao Xu, Jian Zhang, Zheng Wang, Fang Wang, Jinzi Zeng, Zishan Lin, Nanxin Lu, Tao Xu, Jian Zhang, Zheng Wang, Fang Wang, Jinzi |
| collection | PubMed - marine biology |
| contents | Evaluation of Hypovirus Infection on the Vesicular Protein Expression Pattern of by TMT-Based Proteomics Analysis. Zeng, Zishan Lin, Nanxin Lu, Tao Xu, Jian Zhang, Zheng Wang, Fang Wang, Jinzi Hypovirus infection is known to reduce the pathogenicity of , the causative agent of chestnut blight. Isoforms derived from a viral protein p48 have been discovered in host mitochondria and vesicles, which may contribute to virulence attenuation, as reported in earlier work using two-dimensional electrophoresis (2-DE). In this study, a total of 1739 fungal proteins were identified in fungal vesicles through Tandem Mass Tag (TMT)-based quantitative proteomics. The infection of CHV1-EP713 was associated with 75 up-regulated and 201 down-regulated proteins, predominantly involved in vesicular transport process and related cellular functions, including protein folding, membrane fusion, retrograde transport, autophagy, and ER stress responses. The down-regulation of calnexin, COPI, ArfGAP, importin-β, and Atg8 is consistent with impairments in protein folding, retrograde transport, and autophagy. Meanwhile, the up-regulation of clathrin, dynamin, Vps10p, HSP70, and t-SNAREs indicated enhanced trafficking to vacuoles and increased stress response activity. Overall, our findings indicate that hypoviral infection is associated with extensive alterations in the vesicular transport system of , likely mediated through changes in the abundance of multiple key protein regulators. These alterations may underlie attenuation of virulence by impacting crucial cellular processes. |
| format | Artículo científico |
| id | pubmed_41007270 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Biology |
| record_format | pubmed |
| spellingShingle | Evaluation of Hypovirus Infection on the Vesicular Protein Expression Pattern of by TMT-Based Proteomics Analysis. Zeng, Zishan Lin, Nanxin Lu, Tao Xu, Jian Zhang, Zheng Wang, Fang Wang, Jinzi Evaluation of Hypovirus Infection on the Vesicular Protein Expression Pattern of by TMT-Based Proteomics Analysis. Zeng, Zishan Lin, Nanxin Lu, Tao Xu, Jian Zhang, Zheng Wang, Fang Wang, Jinzi Hypovirus infection is known to reduce the pathogenicity of , the causative agent of chestnut blight. Isoforms derived from a viral protein p48 have been discovered in host mitochondria and vesicles, which may contribute to virulence attenuation, as reported in earlier work using two-dimensional electrophoresis (2-DE). In this study, a total of 1739 fungal proteins were identified in fungal vesicles through Tandem Mass Tag (TMT)-based quantitative proteomics. The infection of CHV1-EP713 was associated with 75 up-regulated and 201 down-regulated proteins, predominantly involved in vesicular transport process and related cellular functions, including protein folding, membrane fusion, retrograde transport, autophagy, and ER stress responses. The down-regulation of calnexin, COPI, ArfGAP, importin-β, and Atg8 is consistent with impairments in protein folding, retrograde transport, and autophagy. Meanwhile, the up-regulation of clathrin, dynamin, Vps10p, HSP70, and t-SNAREs indicated enhanced trafficking to vacuoles and increased stress response activity. Overall, our findings indicate that hypoviral infection is associated with extensive alterations in the vesicular transport system of , likely mediated through changes in the abundance of multiple key protein regulators. These alterations may underlie attenuation of virulence by impacting crucial cellular processes. |
| title | Evaluation of Hypovirus Infection on the Vesicular Protein Expression Pattern of by TMT-Based Proteomics Analysis. |
| url | https://pubmed.ncbi.nlm.nih.gov/41007270/ |