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author Gaspar, Adi
Arantes, Larissa S
Ohana, Talya
Bodenheimer, Yair E
Tikochinski, Gili
Levy, Opal
Mor, Bar J
Vainberg, Muriel
Gat, Tomer
Mbedi, Susan
Sparmann, Sarah
Türkozan, Oğuz
Levy, Yaniv
Leader, Noam
Milstein, Dana
Mazzoni, Camila J
Tikochinski, Yaron
author_facet Gaspar, Adi
Arantes, Larissa S
Ohana, Talya
Bodenheimer, Yair E
Tikochinski, Gili
Levy, Opal
Mor, Bar J
Vainberg, Muriel
Gat, Tomer
Mbedi, Susan
Sparmann, Sarah
Türkozan, Oğuz
Levy, Yaniv
Leader, Noam
Milstein, Dana
Mazzoni, Camila J
Tikochinski, Yaron
Gaspar, Adi
Arantes, Larissa S
Ohana, Talya
Bodenheimer, Yair E
Tikochinski, Gili
Levy, Opal
Mor, Bar J
Vainberg, Muriel
Gat, Tomer
Mbedi, Susan
Sparmann, Sarah
Türkozan, Oğuz
Levy, Yaniv
Leader, Noam
Milstein, Dana
Mazzoni, Camila J
Tikochinski, Yaron
collection PubMed - marine biology
contents The Mediterranean Habitat of the Nile Soft-Shelled Turtle (): Genomic and Reproductive Insights into an Endangered Population. Gaspar, Adi Arantes, Larissa S Ohana, Talya Bodenheimer, Yair E Tikochinski, Gili Levy, Opal Mor, Bar J Vainberg, Muriel Gat, Tomer Mbedi, Susan Sparmann, Sarah Türkozan, Oğuz Levy, Yaniv Leader, Noam Milstein, Dana Mazzoni, Camila J Tikochinski, Yaron Ecosystem Mediterranean Region Endangered Species Turtles DNA, Mitochondrial Tandem Repeat Sequences Breeding Polymorphism, Single Nucleotide Maternal Inheritance Population Density Animals Reproduction Gene Flow Paternal Inheritance Male Female Inbreeding Genomics The Mediterranean soft-shell turtle () is classified as critically endangered by the IUCN. Effective conservation requires a clear understanding of its reproductive strategies and population structure. By combining mitochondrial DNA tandem repeat-region profiling with genome-wide SNP data obtained through 3RADseq, we gained high-resolution insights into the genetic composition and breeding behavior of Mediterranean populations. Our results revealed complex reproductive dynamics, including multiple paternity, sperm storage, and repeated nesting within a single season-strategies that enhance genetic diversity in small, fragmented populations. Using SNP-based kinship inference, we estimated the number of breeding females and identified full and half-sibling groups, offering a robust genomic framework for assessing population size and structure. Genetic similarity patterns highlighted moderate differentiation among Israeli river populations, suggesting some connectivity, while samples from Türkiye were clearly distinct, reflecting long-term geographic and genetic separation. This integrative approach provides a scalable, repeatable tool for long-term monitoring. The combined use of maternal and biparental markers enables detailed tracking of genetic diversity, breeding contributions, and demographic trends-key elements for designing informed, adaptive conservation strategies.
format Artículo científico
id pubmed_41009390
institution PubMed
language en
publishDate 2025
publisher International journal of molecular sciences
record_format pubmed
spellingShingle The Mediterranean Habitat of the Nile Soft-Shelled Turtle (): Genomic and Reproductive Insights into an Endangered Population.
Gaspar, Adi
Arantes, Larissa S
Ohana, Talya
Bodenheimer, Yair E
Tikochinski, Gili
Levy, Opal
Mor, Bar J
Vainberg, Muriel
Gat, Tomer
Mbedi, Susan
Sparmann, Sarah
Türkozan, Oğuz
Levy, Yaniv
Leader, Noam
Milstein, Dana
Mazzoni, Camila J
Tikochinski, Yaron
Ecosystem
Mediterranean Region
Endangered Species
Turtles
DNA, Mitochondrial
Tandem Repeat Sequences
Breeding
Polymorphism, Single Nucleotide
Maternal Inheritance
Population Density
Animals
Reproduction
Gene Flow
Paternal Inheritance
Male
Female
Inbreeding
Genomics
The Mediterranean Habitat of the Nile Soft-Shelled Turtle (): Genomic and Reproductive Insights into an Endangered Population. Gaspar, Adi Arantes, Larissa S Ohana, Talya Bodenheimer, Yair E Tikochinski, Gili Levy, Opal Mor, Bar J Vainberg, Muriel Gat, Tomer Mbedi, Susan Sparmann, Sarah Türkozan, Oğuz Levy, Yaniv Leader, Noam Milstein, Dana Mazzoni, Camila J Tikochinski, Yaron Ecosystem Mediterranean Region Endangered Species Turtles DNA, Mitochondrial Tandem Repeat Sequences Breeding Polymorphism, Single Nucleotide Maternal Inheritance Population Density Animals Reproduction Gene Flow Paternal Inheritance Male Female Inbreeding Genomics The Mediterranean soft-shell turtle () is classified as critically endangered by the IUCN. Effective conservation requires a clear understanding of its reproductive strategies and population structure. By combining mitochondrial DNA tandem repeat-region profiling with genome-wide SNP data obtained through 3RADseq, we gained high-resolution insights into the genetic composition and breeding behavior of Mediterranean populations. Our results revealed complex reproductive dynamics, including multiple paternity, sperm storage, and repeated nesting within a single season-strategies that enhance genetic diversity in small, fragmented populations. Using SNP-based kinship inference, we estimated the number of breeding females and identified full and half-sibling groups, offering a robust genomic framework for assessing population size and structure. Genetic similarity patterns highlighted moderate differentiation among Israeli river populations, suggesting some connectivity, while samples from Türkiye were clearly distinct, reflecting long-term geographic and genetic separation. This integrative approach provides a scalable, repeatable tool for long-term monitoring. The combined use of maternal and biparental markers enables detailed tracking of genetic diversity, breeding contributions, and demographic trends-key elements for designing informed, adaptive conservation strategies.
title The Mediterranean Habitat of the Nile Soft-Shelled Turtle (): Genomic and Reproductive Insights into an Endangered Population.
topic Ecosystem
Mediterranean Region
Endangered Species
Turtles
DNA, Mitochondrial
Tandem Repeat Sequences
Breeding
Polymorphism, Single Nucleotide
Maternal Inheritance
Population Density
Animals
Reproduction
Gene Flow
Paternal Inheritance
Male
Female
Inbreeding
Genomics
url https://pubmed.ncbi.nlm.nih.gov/41009390/