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| Format: | Artículo científico |
| Sprache: | en |
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RNA (New York, N.Y.)
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/41027712/ |
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Inhaltsangabe:
- Splice site diversity and abundance of noncanonical introns in diplonemids (Diplonemea, Euglenozoa). Thakur, Prasoon K Butenko, Anzhelika Karásek, Filip Svobodová, Michaela Faktorová, Drahomíra Pavlisková, Hana Varga, Vladimir Horák, Aleš Lukeš, Julius Staněk, David Euglenozoa RNA Splice Sites Introns RNA, Protozoan RNA Splicing Spliceosomes Genes, Protozoan Base Sequence Genetic Variation RNA, Small Nuclear Ribonucleoproteins, Small Nuclear Noncoding introns are a unifying feature of protein-coding genes in virtually all extant eukaryotes, with most lineages following the canonical intron structure. However, euglenozoans, unicellular flagellates that include free-living euglenids, human pathogenic kinetoplastids, and highly diverse and abundant marine diplonemids, are a notable exception. Euglenozoan genomes range from extremely intron-poor kinetoplastids to euglenid genomes containing both canonical and noncanonical introns. Here, we present a comprehensive analysis of splice sites and spliceosomal components in six species of understudied diplonemids. All diplonemids examined contain a nearly complete set of spliceosomal snRNP components, indicating the presence of a functional U2-type spliceosome. However, the majority of introns in the hemistasiid diplonemids and are noncanonical and lack conserved GT-AG terminal dinucleotides typical for U2-type introns. These noncanonical introns are capable of extensive base-pairing, which brings intron ends into close proximity. Thus, while the splicing apparatus is conserved in diplonemids, the splice sites are highly variable among individual species.