Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
FEMS microbiology letters
2025
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41032321/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266146693644288 |
|---|---|
| author | Fan, Wenqi Zhang, Dengwei Li, Yuqing Wang, Haiyan Li, Yong-Xin Li, Bin Zou, Shien Feng, Chenchen Guo, Ting |
| author_facet | Fan, Wenqi Zhang, Dengwei Li, Yuqing Wang, Haiyan Li, Yong-Xin Li, Bin Zou, Shien Feng, Chenchen Guo, Ting Fan, Wenqi Zhang, Dengwei Li, Yuqing Wang, Haiyan Li, Yong-Xin Li, Bin Zou, Shien Feng, Chenchen Guo, Ting |
| collection | PubMed - marine biology |
| contents | Intratumoral fungus Neurospora crassa is associated with worsened prognosis in ovarian cancer via modulation of extracellular matrix. Fan, Wenqi Zhang, Dengwei Li, Yuqing Wang, Haiyan Li, Yong-Xin Li, Bin Zou, Shien Feng, Chenchen Guo, Ting Neurospora crassa Mycoses Prognosis Ovarian Neoplasms Humans Female Animals Mice Cell Line, Tumor Extracellular Matrix Eosinophils Landmark studies on intratumoral fungi (ITF) have raised concerns due to irreproducible results and data-analysis errors. We aimed to determine whether ITF exist in ovarian cancer (OvCa) and, if so, whether they play a role in disease biology. Formalin-fixed, paraffin-embedded OvCa samples and multiple controls underwent operational decontamination, qPCR, internal transcribed spacer sequencing, and post-hoc data decontamination. We also leveraged updated fungal reads from The Cancer Genome Atlas generated by the TCMbio group, which addressed human-read contamination and artificial inflation, to validate findings and assess prognostic associations. A murine syngeneic model established using mouse ovarian cancer cell line (OVHM) with intratumoral Neurospora crassa injection was established. Transcriptomic and metabolomic analyses were performed to explore mechanisms. Tumor-containing blocks harbored significantly higher fungal loads than environmental controls but had loads comparable to paraffin controls. Applying a two-pass decontamination filter reduced raw sequence features from 9289 amplicon sequence variants (ASVs) to 659 ASVs. We focused on high-abundance features present in human tissues but absent from xenografts and paraffin controls and identified one candidate, N. crassa, associated with unfavorable prognosis in OvCa. Integrating human and murine data, we found Neurospora correlated with eosinophils, whereas N. crassa itself was not immune-related. Neurospora crassa promoted OvCa progression with downregulation of integrin-linked kinase and decreased extracellular matrix-receptor interaction. Most ITF signals are likely contaminants. We identified N. crassa as associated with unfavorable prognosis in OvCa, potentially via modulation of the extracellular matrix. |
| format | Artículo científico |
| id | pubmed_41032321 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | FEMS microbiology letters |
| record_format | pubmed |
| spellingShingle | Intratumoral fungus Neurospora crassa is associated with worsened prognosis in ovarian cancer via modulation of extracellular matrix. Fan, Wenqi Zhang, Dengwei Li, Yuqing Wang, Haiyan Li, Yong-Xin Li, Bin Zou, Shien Feng, Chenchen Guo, Ting Neurospora crassa Mycoses Prognosis Ovarian Neoplasms Humans Female Animals Mice Cell Line, Tumor Extracellular Matrix Eosinophils Intratumoral fungus Neurospora crassa is associated with worsened prognosis in ovarian cancer via modulation of extracellular matrix. Fan, Wenqi Zhang, Dengwei Li, Yuqing Wang, Haiyan Li, Yong-Xin Li, Bin Zou, Shien Feng, Chenchen Guo, Ting Neurospora crassa Mycoses Prognosis Ovarian Neoplasms Humans Female Animals Mice Cell Line, Tumor Extracellular Matrix Eosinophils Landmark studies on intratumoral fungi (ITF) have raised concerns due to irreproducible results and data-analysis errors. We aimed to determine whether ITF exist in ovarian cancer (OvCa) and, if so, whether they play a role in disease biology. Formalin-fixed, paraffin-embedded OvCa samples and multiple controls underwent operational decontamination, qPCR, internal transcribed spacer sequencing, and post-hoc data decontamination. We also leveraged updated fungal reads from The Cancer Genome Atlas generated by the TCMbio group, which addressed human-read contamination and artificial inflation, to validate findings and assess prognostic associations. A murine syngeneic model established using mouse ovarian cancer cell line (OVHM) with intratumoral Neurospora crassa injection was established. Transcriptomic and metabolomic analyses were performed to explore mechanisms. Tumor-containing blocks harbored significantly higher fungal loads than environmental controls but had loads comparable to paraffin controls. Applying a two-pass decontamination filter reduced raw sequence features from 9289 amplicon sequence variants (ASVs) to 659 ASVs. We focused on high-abundance features present in human tissues but absent from xenografts and paraffin controls and identified one candidate, N. crassa, associated with unfavorable prognosis in OvCa. Integrating human and murine data, we found Neurospora correlated with eosinophils, whereas N. crassa itself was not immune-related. Neurospora crassa promoted OvCa progression with downregulation of integrin-linked kinase and decreased extracellular matrix-receptor interaction. Most ITF signals are likely contaminants. We identified N. crassa as associated with unfavorable prognosis in OvCa, potentially via modulation of the extracellular matrix. |
| title | Intratumoral fungus Neurospora crassa is associated with worsened prognosis in ovarian cancer via modulation of extracellular matrix. |
| topic | Neurospora crassa Mycoses Prognosis Ovarian Neoplasms Humans Female Animals Mice Cell Line, Tumor Extracellular Matrix Eosinophils |
| url | https://pubmed.ncbi.nlm.nih.gov/41032321/ |