Integrated physiological and transcriptomic studies on the effects of melatonin on carotenoid biosynthesis in Chlamydomonas reinhardtii under chromium stress.
Fuente:
PubMed
Salvato in:
| Autori principali: | , , , , , |
|---|---|
| Natura: | Artículo científico |
| Lingua: | en |
| Pubblicazione: |
Plant physiology and biochemistry : PPB
2025
|
| Soggetti: | |
| Accesso online: | |
| Tags: |
Aggiungi Tag
Nessun Tag, puoi essere il primo ad aggiungerne!!
|
| _version_ | 1868266144191741953 |
|---|---|
| author | Yan, Meilin Mo, Jiawei Huang, Can Li, Jianing Yao, Xiangyu Wang, Yingjuan |
| author_facet | Yan, Meilin Mo, Jiawei Huang, Can Li, Jianing Yao, Xiangyu Wang, Yingjuan Yan, Meilin Mo, Jiawei Huang, Can Li, Jianing Yao, Xiangyu Wang, Yingjuan |
| collection | PubMed - marine biology |
| contents | Integrated physiological and transcriptomic studies on the effects of melatonin on carotenoid biosynthesis in Chlamydomonas reinhardtii under chromium stress. Yan, Meilin Mo, Jiawei Huang, Can Li, Jianing Yao, Xiangyu Wang, Yingjuan Melatonin Chlamydomonas reinhardtii Carotenoids Chromium Transcriptome Stress, Physiological Gene Expression Profiling Gene Expression Regulation, Plant Chromium stress threatens marine organisms, disrupts food webs, and contaminates river water, endangering human and agricultural uses. It also impacts the growth and physiology of the model alga Chlamydomonas reinhardtii, which serves as a sensitive environmental indicator due to its measurable responses to pollution. Melatonin can mitigate abiotic stresses in various organisms. This study uses integrated physiological and transcriptomic approaches to explore melatonin's regulatory effects on carotenoid biosynthesis in chromium-stressed C. reinhardtii. Four conditions were studied: CK (control), MT (1 μM melatonin), Cr (0.01 μmol/L chromium), and Cr + MT. Physiological assessments covered growth, pigment content, osmolytes, antioxidant systems, and photosynthetic performance. Results showed that melatonin alleviated chromium-induced growth inhibition and pigment changes. Transcriptomic analysis identified differentially expressed genes (DEGs) in carotenoid biosynthesis pathways. KEGG pathway classification and enrichment analyses revealed significant pathway alterations. qPCR validated differential regulation of key carotenogenic genes (DXS, DXR, PSY, and PDS) in the Cr + MT treatment. These findings offer comprehensive insights into melatonin-mediated protection of carotenoid biosynthesis against chromium stress, with implications for understanding microalgal stress responses and applications in bioremediation and biotechnology. |
| format | Artículo científico |
| id | pubmed_41043368 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Plant physiology and biochemistry : PPB |
| record_format | pubmed |
| spellingShingle | Integrated physiological and transcriptomic studies on the effects of melatonin on carotenoid biosynthesis in Chlamydomonas reinhardtii under chromium stress. Yan, Meilin Mo, Jiawei Huang, Can Li, Jianing Yao, Xiangyu Wang, Yingjuan Melatonin Chlamydomonas reinhardtii Carotenoids Chromium Transcriptome Stress, Physiological Gene Expression Profiling Gene Expression Regulation, Plant Integrated physiological and transcriptomic studies on the effects of melatonin on carotenoid biosynthesis in Chlamydomonas reinhardtii under chromium stress. Yan, Meilin Mo, Jiawei Huang, Can Li, Jianing Yao, Xiangyu Wang, Yingjuan Melatonin Chlamydomonas reinhardtii Carotenoids Chromium Transcriptome Stress, Physiological Gene Expression Profiling Gene Expression Regulation, Plant Chromium stress threatens marine organisms, disrupts food webs, and contaminates river water, endangering human and agricultural uses. It also impacts the growth and physiology of the model alga Chlamydomonas reinhardtii, which serves as a sensitive environmental indicator due to its measurable responses to pollution. Melatonin can mitigate abiotic stresses in various organisms. This study uses integrated physiological and transcriptomic approaches to explore melatonin's regulatory effects on carotenoid biosynthesis in chromium-stressed C. reinhardtii. Four conditions were studied: CK (control), MT (1 μM melatonin), Cr (0.01 μmol/L chromium), and Cr + MT. Physiological assessments covered growth, pigment content, osmolytes, antioxidant systems, and photosynthetic performance. Results showed that melatonin alleviated chromium-induced growth inhibition and pigment changes. Transcriptomic analysis identified differentially expressed genes (DEGs) in carotenoid biosynthesis pathways. KEGG pathway classification and enrichment analyses revealed significant pathway alterations. qPCR validated differential regulation of key carotenogenic genes (DXS, DXR, PSY, and PDS) in the Cr + MT treatment. These findings offer comprehensive insights into melatonin-mediated protection of carotenoid biosynthesis against chromium stress, with implications for understanding microalgal stress responses and applications in bioremediation and biotechnology. |
| title | Integrated physiological and transcriptomic studies on the effects of melatonin on carotenoid biosynthesis in Chlamydomonas reinhardtii under chromium stress. |
| topic | Melatonin Chlamydomonas reinhardtii Carotenoids Chromium Transcriptome Stress, Physiological Gene Expression Profiling Gene Expression Regulation, Plant |
| url | https://pubmed.ncbi.nlm.nih.gov/41043368/ |