Enhancing isoprimeverose-producing enzyme in genetically engineered Aspergillus oryzae through impeller shape and pH control for the large-scale fermentation.
Fuente:
PubMed
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Enzyme and microbial technology
2026
|
| Subjects: | |
| Online Access: | |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266141761142787 |
|---|---|
| author | Baihaqqi, Fahmi Wakai, Satoshi Putra, Filemon Jalu Nusantara Suzuki, Tomohiro Ikeya, Yoshiro Kahar, Prihardi Mori, Yutaro Kondo, Akihiko Ogino, Chiaki |
| author_facet | Baihaqqi, Fahmi Wakai, Satoshi Putra, Filemon Jalu Nusantara Suzuki, Tomohiro Ikeya, Yoshiro Kahar, Prihardi Mori, Yutaro Kondo, Akihiko Ogino, Chiaki Baihaqqi, Fahmi Wakai, Satoshi Putra, Filemon Jalu Nusantara Suzuki, Tomohiro Ikeya, Yoshiro Kahar, Prihardi Mori, Yutaro Kondo, Akihiko Ogino, Chiaki |
| collection | PubMed - marine biology |
| contents | Enhancing isoprimeverose-producing enzyme in genetically engineered Aspergillus oryzae through impeller shape and pH control for the large-scale fermentation. Baihaqqi, Fahmi Wakai, Satoshi Putra, Filemon Jalu Nusantara Suzuki, Tomohiro Ikeya, Yoshiro Kahar, Prihardi Mori, Yutaro Kondo, Akihiko Ogino, Chiaki Aspergillus oryzae Fermentation Hydrogen-Ion Concentration Bioreactors Cellulase Maltose Fungal Proteins Glucose Genetic Engineering Disaccharides Culture Media Metabolic Engineering Glucans Glycoside Hydrolases Aspergillus oryzae is a filamentous fungus that serves as a source of polysaccharide-degrading enzymes. We constructed two self-cloning forms of A. oryzae that specifically express isoprimeverose oligoxyloglucanase (IpeA) and endoglucanase. These enzymes are essential for the enzymatic release of the rare sugar isoprimeverose (α-D-xylopyranosyl-(1→6)-D-glucopyranose) from tamarind xyloglucan. Of the two, IpeA is a key enzyme that is currently unavailable commercially. The primary objective of this study was to enhance IpeA production through fermentation engineering. Various fermentation parameters were optimized. These include a source of carbon from either glucose or maltose, pH control, and impeller shapes using either Disk Turbine (DT) or MAXBLEND® (MB) impellers that were tested using a 5-L stirred-tank bioreactor. Cultivation in maltose-supplemented medium resulted in a reduction in carbon catabolite repression compared with that of glucose. Batch fermentation at acidic pH (4.5) using the MB impeller yielded a significantly higher level of biomass and enzyme production with a dry mycelial weight (DMW) yield of ∼19 g/L and IpeA activity of ∼68 U/mL, which compares favorably with ∼14 g/L of DMW and 43 U/mL of IpeA activity under identical pH conditions when using a DT impeller. The findings of this study offer valuable insight for both large-scale and biological production of A. oryzae. |
| format | Artículo científico |
| id | pubmed_41072238 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Enzyme and microbial technology |
| record_format | pubmed |
| spellingShingle | Enhancing isoprimeverose-producing enzyme in genetically engineered Aspergillus oryzae through impeller shape and pH control for the large-scale fermentation. Baihaqqi, Fahmi Wakai, Satoshi Putra, Filemon Jalu Nusantara Suzuki, Tomohiro Ikeya, Yoshiro Kahar, Prihardi Mori, Yutaro Kondo, Akihiko Ogino, Chiaki Aspergillus oryzae Fermentation Hydrogen-Ion Concentration Bioreactors Cellulase Maltose Fungal Proteins Glucose Genetic Engineering Disaccharides Culture Media Metabolic Engineering Glucans Glycoside Hydrolases Enhancing isoprimeverose-producing enzyme in genetically engineered Aspergillus oryzae through impeller shape and pH control for the large-scale fermentation. Baihaqqi, Fahmi Wakai, Satoshi Putra, Filemon Jalu Nusantara Suzuki, Tomohiro Ikeya, Yoshiro Kahar, Prihardi Mori, Yutaro Kondo, Akihiko Ogino, Chiaki Aspergillus oryzae Fermentation Hydrogen-Ion Concentration Bioreactors Cellulase Maltose Fungal Proteins Glucose Genetic Engineering Disaccharides Culture Media Metabolic Engineering Glucans Glycoside Hydrolases Aspergillus oryzae is a filamentous fungus that serves as a source of polysaccharide-degrading enzymes. We constructed two self-cloning forms of A. oryzae that specifically express isoprimeverose oligoxyloglucanase (IpeA) and endoglucanase. These enzymes are essential for the enzymatic release of the rare sugar isoprimeverose (α-D-xylopyranosyl-(1→6)-D-glucopyranose) from tamarind xyloglucan. Of the two, IpeA is a key enzyme that is currently unavailable commercially. The primary objective of this study was to enhance IpeA production through fermentation engineering. Various fermentation parameters were optimized. These include a source of carbon from either glucose or maltose, pH control, and impeller shapes using either Disk Turbine (DT) or MAXBLEND® (MB) impellers that were tested using a 5-L stirred-tank bioreactor. Cultivation in maltose-supplemented medium resulted in a reduction in carbon catabolite repression compared with that of glucose. Batch fermentation at acidic pH (4.5) using the MB impeller yielded a significantly higher level of biomass and enzyme production with a dry mycelial weight (DMW) yield of ∼19 g/L and IpeA activity of ∼68 U/mL, which compares favorably with ∼14 g/L of DMW and 43 U/mL of IpeA activity under identical pH conditions when using a DT impeller. The findings of this study offer valuable insight for both large-scale and biological production of A. oryzae. |
| title | Enhancing isoprimeverose-producing enzyme in genetically engineered Aspergillus oryzae through impeller shape and pH control for the large-scale fermentation. |
| topic | Aspergillus oryzae Fermentation Hydrogen-Ion Concentration Bioreactors Cellulase Maltose Fungal Proteins Glucose Genetic Engineering Disaccharides Culture Media Metabolic Engineering Glucans Glycoside Hydrolases |
| url | https://pubmed.ncbi.nlm.nih.gov/41072238/ |