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| Autores principales: | , , , , , , , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Nature communications
2025
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| Materias: | |
| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/41073418/ |
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| _version_ | 1868266141760094209 |
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| author | Diao, Zhidian Peng, Qiqun Luo, Sijun Kan, Lingyan Ge, Anle Gao, Wei Li, Runxia Bao, Weiwei Wang, Xixian Ji, Yuetong Xu, Jian Yang, Shihui Ma, Bo |
| author_facet | Diao, Zhidian Peng, Qiqun Luo, Sijun Kan, Lingyan Ge, Anle Gao, Wei Li, Runxia Bao, Weiwei Wang, Xixian Ji, Yuetong Xu, Jian Yang, Shihui Ma, Bo Diao, Zhidian Peng, Qiqun Luo, Sijun Kan, Lingyan Ge, Anle Gao, Wei Li, Runxia Bao, Weiwei Wang, Xixian Ji, Yuetong Xu, Jian Yang, Shihui Ma, Bo |
| collection | PubMed - marine biology |
| contents | AI-powered high-throughput digital colony picker platform for sorting microbial strains by multi-modal phenotypes. Diao, Zhidian Peng, Qiqun Luo, Sijun Kan, Lingyan Ge, Anle Gao, Wei Li, Runxia Bao, Weiwei Wang, Xixian Ji, Yuetong Xu, Jian Yang, Shihui Ma, Bo Phenotype High-Throughput Screening Assays Lactic Acid Zymomonas Bacterial Proteins Lab-On-A-Chip Devices Phenotype-based screening remains a major bottleneck in the development of microbial cell factories. Here, we present a Digital Colony Picker (DCP), an AI-powered platform for automated, high-throughput screening and export of microbial clones based on growth and metabolic phenotypes at single-cell resolution, without agar or physical contact. Using a microfluidic chip comprising 16,000 addressable picoliter-scale microchambers, individual cells are compartmentalized, dynamically monitored by AI-driven image analysis, and selectively exported via laser-induced bubble technique. Applied to Zymomonas mobilis, DCP enabled en masse screening and identified a mutant with 19.7% increased lactate production and 77.0% enhanced growth under 30 g/L lactate stress. This phenotype was linked to overexpression of ZMOp39x027, a canonical outer membrane autotransporter that promotes lactate transport and cell proliferation under stress. DCP provides a multi-modal phenotyping solution with spatiotemporal precision and scalable throughput, offering a generalizable strategy for accelerated strain engineering and functional gene discovery. |
| format | Artículo científico |
| id | pubmed_41073418 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Nature communications |
| record_format | pubmed |
| spellingShingle | AI-powered high-throughput digital colony picker platform for sorting microbial strains by multi-modal phenotypes. Diao, Zhidian Peng, Qiqun Luo, Sijun Kan, Lingyan Ge, Anle Gao, Wei Li, Runxia Bao, Weiwei Wang, Xixian Ji, Yuetong Xu, Jian Yang, Shihui Ma, Bo Phenotype High-Throughput Screening Assays Lactic Acid Zymomonas Bacterial Proteins Lab-On-A-Chip Devices AI-powered high-throughput digital colony picker platform for sorting microbial strains by multi-modal phenotypes. Diao, Zhidian Peng, Qiqun Luo, Sijun Kan, Lingyan Ge, Anle Gao, Wei Li, Runxia Bao, Weiwei Wang, Xixian Ji, Yuetong Xu, Jian Yang, Shihui Ma, Bo Phenotype High-Throughput Screening Assays Lactic Acid Zymomonas Bacterial Proteins Lab-On-A-Chip Devices Phenotype-based screening remains a major bottleneck in the development of microbial cell factories. Here, we present a Digital Colony Picker (DCP), an AI-powered platform for automated, high-throughput screening and export of microbial clones based on growth and metabolic phenotypes at single-cell resolution, without agar or physical contact. Using a microfluidic chip comprising 16,000 addressable picoliter-scale microchambers, individual cells are compartmentalized, dynamically monitored by AI-driven image analysis, and selectively exported via laser-induced bubble technique. Applied to Zymomonas mobilis, DCP enabled en masse screening and identified a mutant with 19.7% increased lactate production and 77.0% enhanced growth under 30 g/L lactate stress. This phenotype was linked to overexpression of ZMOp39x027, a canonical outer membrane autotransporter that promotes lactate transport and cell proliferation under stress. DCP provides a multi-modal phenotyping solution with spatiotemporal precision and scalable throughput, offering a generalizable strategy for accelerated strain engineering and functional gene discovery. |
| title | AI-powered high-throughput digital colony picker platform for sorting microbial strains by multi-modal phenotypes. |
| topic | Phenotype High-Throughput Screening Assays Lactic Acid Zymomonas Bacterial Proteins Lab-On-A-Chip Devices |
| url | https://pubmed.ncbi.nlm.nih.gov/41073418/ |