The ω6 Pathway toward Eicosapentaenoic Acid Biosynthesis outside the Chloroplast in the Industrially Relevant Alga .
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| Natura: | Artículo científico |
| Lingua: | en |
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Journal of agricultural and food chemistry
2025
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| _version_ | 1868266141759045632 |
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| author | Zheng, Jie Shi, Ying Liang, Zhiwei Sun, Han Zhou, Wenguang Hu, Hanhua Liu, Jin |
| author_facet | Zheng, Jie Shi, Ying Liang, Zhiwei Sun, Han Zhou, Wenguang Hu, Hanhua Liu, Jin Zheng, Jie Shi, Ying Liang, Zhiwei Sun, Han Zhou, Wenguang Hu, Hanhua Liu, Jin |
| collection | PubMed - marine biology |
| contents | The ω6 Pathway toward Eicosapentaenoic Acid Biosynthesis outside the Chloroplast in the Industrially Relevant Alga . Zheng, Jie Shi, Ying Liang, Zhiwei Sun, Han Zhou, Wenguang Hu, Hanhua Liu, Jin Eicosapentaenoic Acid Stramenopiles Fatty Acid Desaturases Chloroplasts Microalgae Biosynthetic Pathways Fatty Acids, Omega-6 is an emerging chassis for industrial eicosapentaenoic acid (EPA) production, yet its biosynthesis pathway remains incompletely defined. Here, we delineate the pathway by characterizing three key enzymes: Δ12 fatty acid desaturase (NoΔ12FAD), Δ6 fatty acid desaturase (NoΔ6FAD), and Δ5 fatty acid desaturase (NoΔ5FAD). Functional validation in yeast confirmed their specific desaturation activities, and localization experiments showed they reside outside the plastid of . Genetic manipulation of individual genes (knockdown/overexpression) reshaped fatty acid profiles, particularly EPA, while having a minimal effect on the transcription of other EPA biosynthesis genes. Moreover, impairing EPA biosynthesis via knockdown diminished the alga's resilience to temperature and light stress, underscoring the role of EPA in stress adaptation. Collectively, our findings offer new insights into EPA biosynthesis in , highlighting its production through the ω6 pathway via sequential desaturation and elongation steps outside of the chloroplast. |
| format | Artículo científico |
| id | pubmed_41074840 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Journal of agricultural and food chemistry |
| record_format | pubmed |
| spellingShingle | The ω6 Pathway toward Eicosapentaenoic Acid Biosynthesis outside the Chloroplast in the Industrially Relevant Alga . Zheng, Jie Shi, Ying Liang, Zhiwei Sun, Han Zhou, Wenguang Hu, Hanhua Liu, Jin Eicosapentaenoic Acid Stramenopiles Fatty Acid Desaturases Chloroplasts Microalgae Biosynthetic Pathways Fatty Acids, Omega-6 The ω6 Pathway toward Eicosapentaenoic Acid Biosynthesis outside the Chloroplast in the Industrially Relevant Alga . Zheng, Jie Shi, Ying Liang, Zhiwei Sun, Han Zhou, Wenguang Hu, Hanhua Liu, Jin Eicosapentaenoic Acid Stramenopiles Fatty Acid Desaturases Chloroplasts Microalgae Biosynthetic Pathways Fatty Acids, Omega-6 is an emerging chassis for industrial eicosapentaenoic acid (EPA) production, yet its biosynthesis pathway remains incompletely defined. Here, we delineate the pathway by characterizing three key enzymes: Δ12 fatty acid desaturase (NoΔ12FAD), Δ6 fatty acid desaturase (NoΔ6FAD), and Δ5 fatty acid desaturase (NoΔ5FAD). Functional validation in yeast confirmed their specific desaturation activities, and localization experiments showed they reside outside the plastid of . Genetic manipulation of individual genes (knockdown/overexpression) reshaped fatty acid profiles, particularly EPA, while having a minimal effect on the transcription of other EPA biosynthesis genes. Moreover, impairing EPA biosynthesis via knockdown diminished the alga's resilience to temperature and light stress, underscoring the role of EPA in stress adaptation. Collectively, our findings offer new insights into EPA biosynthesis in , highlighting its production through the ω6 pathway via sequential desaturation and elongation steps outside of the chloroplast. |
| title | The ω6 Pathway toward Eicosapentaenoic Acid Biosynthesis outside the Chloroplast in the Industrially Relevant Alga . |
| topic | Eicosapentaenoic Acid Stramenopiles Fatty Acid Desaturases Chloroplasts Microalgae Biosynthetic Pathways Fatty Acids, Omega-6 |
| url | https://pubmed.ncbi.nlm.nih.gov/41074840/ |