Okadaic acid induces oxidative stress and immunotoxicity in zebrafish: involvement of the p53 pathway and behavioral alterations.
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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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Fish & shellfish immunology
2026
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| _version_ | 1868266141734928384 |
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| author | Chen, Duo Pan, Siming Wang, Jiapeng Li, Zhenlu Dong, Chenhuan Liang, Yuanxin Huang, Yilin Chen, Youqiang Xue, Ting Lin, Chentao |
| author_facet | Chen, Duo Pan, Siming Wang, Jiapeng Li, Zhenlu Dong, Chenhuan Liang, Yuanxin Huang, Yilin Chen, Youqiang Xue, Ting Lin, Chentao Chen, Duo Pan, Siming Wang, Jiapeng Li, Zhenlu Dong, Chenhuan Liang, Yuanxin Huang, Yilin Chen, Youqiang Xue, Ting Lin, Chentao |
| collection | PubMed - marine biology |
| contents | Okadaic acid induces oxidative stress and immunotoxicity in zebrafish: involvement of the p53 pathway and behavioral alterations. Chen, Duo Pan, Siming Wang, Jiapeng Li, Zhenlu Dong, Chenhuan Liang, Yuanxin Huang, Yilin Chen, Youqiang Xue, Ting Lin, Chentao Animals Zebrafish Oxidative Stress Tumor Suppressor Protein p53 Okadaic Acid Behavior, Animal Apoptosis Zebrafish Proteins Signal Transduction Mice Cell Line Marine Toxins Okadaic acid (OA), a marine phycotoxin, was examined under a host-centric, mechanism-oriented framework using zebrafish (Danio rerio) as the primary vertebrate model and murine C2C12 myoblasts as a muscle-specific mechanistic support. Sub-lethal, pathway-probing exposures were employed to elicit interpretable endpoints without recreating environmental concentrations. Following intraperitoneal OA administration, zebrafish exhibited concentration- and time-dependent reductions in locomotor performance, which were accompanied by biochemical evidence of redox disequilibrium, including elevated reactive oxygen species and perturbed antioxidant defenses. Transcriptomic and molecular analyses indicated engagement of immune-associated signaling together with activation of a p53-linked apoptotic program, characterized by the time-resolved modulation of canonical pro- and anti-apoptotic markers. In parallel, C2C12 assays conducted at sub-lethal levels demonstrated oxidative stress, mitochondrial involvement, and induction of apoptosis, thereby providing a muscle-contextual mechanistic alignment with organism-level observations. Taken together, the data are consistent with a cascade in which OA-induced oxidative stress precedes p53 activation and apoptotic execution and is accompanied by graded behavioral impairment in vivo. The cellular findings are interpreted strictly as mechanistic support and are not used for quantitative cross-species extrapolation. By integrating molecular, cellular, and behavioral readouts in a single vertebrate system, this study delineates a testable pathway linking redox stress to p53-associated apoptosis and organismal function, and it situates the interpretation within fish immunology and host biology under sub-lethal exposure conditions. |
| format | Artículo científico |
| id | pubmed_41093255 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Fish & shellfish immunology |
| record_format | pubmed |
| spellingShingle | Okadaic acid induces oxidative stress and immunotoxicity in zebrafish: involvement of the p53 pathway and behavioral alterations. Chen, Duo Pan, Siming Wang, Jiapeng Li, Zhenlu Dong, Chenhuan Liang, Yuanxin Huang, Yilin Chen, Youqiang Xue, Ting Lin, Chentao Animals Zebrafish Oxidative Stress Tumor Suppressor Protein p53 Okadaic Acid Behavior, Animal Apoptosis Zebrafish Proteins Signal Transduction Mice Cell Line Marine Toxins Okadaic acid induces oxidative stress and immunotoxicity in zebrafish: involvement of the p53 pathway and behavioral alterations. Chen, Duo Pan, Siming Wang, Jiapeng Li, Zhenlu Dong, Chenhuan Liang, Yuanxin Huang, Yilin Chen, Youqiang Xue, Ting Lin, Chentao Animals Zebrafish Oxidative Stress Tumor Suppressor Protein p53 Okadaic Acid Behavior, Animal Apoptosis Zebrafish Proteins Signal Transduction Mice Cell Line Marine Toxins Okadaic acid (OA), a marine phycotoxin, was examined under a host-centric, mechanism-oriented framework using zebrafish (Danio rerio) as the primary vertebrate model and murine C2C12 myoblasts as a muscle-specific mechanistic support. Sub-lethal, pathway-probing exposures were employed to elicit interpretable endpoints without recreating environmental concentrations. Following intraperitoneal OA administration, zebrafish exhibited concentration- and time-dependent reductions in locomotor performance, which were accompanied by biochemical evidence of redox disequilibrium, including elevated reactive oxygen species and perturbed antioxidant defenses. Transcriptomic and molecular analyses indicated engagement of immune-associated signaling together with activation of a p53-linked apoptotic program, characterized by the time-resolved modulation of canonical pro- and anti-apoptotic markers. In parallel, C2C12 assays conducted at sub-lethal levels demonstrated oxidative stress, mitochondrial involvement, and induction of apoptosis, thereby providing a muscle-contextual mechanistic alignment with organism-level observations. Taken together, the data are consistent with a cascade in which OA-induced oxidative stress precedes p53 activation and apoptotic execution and is accompanied by graded behavioral impairment in vivo. The cellular findings are interpreted strictly as mechanistic support and are not used for quantitative cross-species extrapolation. By integrating molecular, cellular, and behavioral readouts in a single vertebrate system, this study delineates a testable pathway linking redox stress to p53-associated apoptosis and organismal function, and it situates the interpretation within fish immunology and host biology under sub-lethal exposure conditions. |
| title | Okadaic acid induces oxidative stress and immunotoxicity in zebrafish: involvement of the p53 pathway and behavioral alterations. |
| topic | Animals Zebrafish Oxidative Stress Tumor Suppressor Protein p53 Okadaic Acid Behavior, Animal Apoptosis Zebrafish Proteins Signal Transduction Mice Cell Line Marine Toxins |
| url | https://pubmed.ncbi.nlm.nih.gov/41093255/ |