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| Format: | Artículo científico |
| Langue: | en |
| Publié: |
Scientific reports
2025
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| Accès en ligne: | https://pubmed.ncbi.nlm.nih.gov/41102233/ |
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| author | Kwak, Min-Jung Park, Jaehyo Park, Hyein Yoon, Jaekyung Lee, Jidam Hahnke, Richard L Lee, Seon-Woo Kwon, Soon-Kyeong Song, Ju Yeon Kim, Jihyun F |
| author_facet | Kwak, Min-Jung Park, Jaehyo Park, Hyein Yoon, Jaekyung Lee, Jidam Hahnke, Richard L Lee, Seon-Woo Kwon, Soon-Kyeong Song, Ju Yeon Kim, Jihyun F Kwak, Min-Jung Park, Jaehyo Park, Hyein Yoon, Jaekyung Lee, Jidam Hahnke, Richard L Lee, Seon-Woo Kwon, Soon-Kyeong Song, Ju Yeon Kim, Jihyun F |
| collection | PubMed - marine biology |
| contents | Polyphasic and comparative genomic characterization of a novel Mariniflexile species in the rhizosphere microbiome of tomato resistant to bacterial wilt. Kwak, Min-Jung Park, Jaehyo Park, Hyein Yoon, Jaekyung Lee, Jidam Hahnke, Richard L Lee, Seon-Woo Kwon, Soon-Kyeong Song, Ju Yeon Kim, Jihyun F Solanum lycopersicum Rhizosphere Phylogeny Plant Diseases RNA, Ribosomal, 16S Microbiota Genome, Bacterial Ralstonia solanacearum Soil Microbiology Genomics Disease Resistance A plethora of microbes resides in the plant rhizosphere, and some play roles in host health and disease. We previously isolated a Gram-negative, aerobic, rod-shaped rhizobacterium, TRM1-10, that contributes to bacterial wilt resistance of tomato caused by Ralstonia solanacearum. In this study, we characterized TRM1-10 through physiological and biochemical analyses, complemented by whole genome sequencing and comparative genomic analyses. Phylogenetic analysis using the 16S rRNA gene and genome sequences revealed that TRM1-10 belongs to the genus Mariniflexile and represents a new lineage. TRM1-10 also exhibits noticeable differences in physiological and biochemical characteristics compared to other Mariniflexile species. Thus, based on phylogenetic affiliation and chemotaxonomic characteristics, we propose this bacterium as a novel species in the genus, Mariniflexile rhizosphaerae sp. nov. (type strain TRM1-10 = KCTC 18646P = DSM 33122). Comparative genome analyses revealed that TRM1-10 harbors more genes linked to soil adaptation compared to other phylogenetically related Mariniflexile species, most of which are associated with marine habitats. The genomic features of TRM1-10 and other strains in the species may allow the taxon to adapt to the soil and rhizosphere, compete effectively with the resident soil microbiota, and contribute to plant health. |
| format | Artículo científico |
| id | pubmed_41102233 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Scientific reports |
| record_format | pubmed |
| spellingShingle | Polyphasic and comparative genomic characterization of a novel Mariniflexile species in the rhizosphere microbiome of tomato resistant to bacterial wilt. Kwak, Min-Jung Park, Jaehyo Park, Hyein Yoon, Jaekyung Lee, Jidam Hahnke, Richard L Lee, Seon-Woo Kwon, Soon-Kyeong Song, Ju Yeon Kim, Jihyun F Solanum lycopersicum Rhizosphere Phylogeny Plant Diseases RNA, Ribosomal, 16S Microbiota Genome, Bacterial Ralstonia solanacearum Soil Microbiology Genomics Disease Resistance Polyphasic and comparative genomic characterization of a novel Mariniflexile species in the rhizosphere microbiome of tomato resistant to bacterial wilt. Kwak, Min-Jung Park, Jaehyo Park, Hyein Yoon, Jaekyung Lee, Jidam Hahnke, Richard L Lee, Seon-Woo Kwon, Soon-Kyeong Song, Ju Yeon Kim, Jihyun F Solanum lycopersicum Rhizosphere Phylogeny Plant Diseases RNA, Ribosomal, 16S Microbiota Genome, Bacterial Ralstonia solanacearum Soil Microbiology Genomics Disease Resistance A plethora of microbes resides in the plant rhizosphere, and some play roles in host health and disease. We previously isolated a Gram-negative, aerobic, rod-shaped rhizobacterium, TRM1-10, that contributes to bacterial wilt resistance of tomato caused by Ralstonia solanacearum. In this study, we characterized TRM1-10 through physiological and biochemical analyses, complemented by whole genome sequencing and comparative genomic analyses. Phylogenetic analysis using the 16S rRNA gene and genome sequences revealed that TRM1-10 belongs to the genus Mariniflexile and represents a new lineage. TRM1-10 also exhibits noticeable differences in physiological and biochemical characteristics compared to other Mariniflexile species. Thus, based on phylogenetic affiliation and chemotaxonomic characteristics, we propose this bacterium as a novel species in the genus, Mariniflexile rhizosphaerae sp. nov. (type strain TRM1-10 = KCTC 18646P = DSM 33122). Comparative genome analyses revealed that TRM1-10 harbors more genes linked to soil adaptation compared to other phylogenetically related Mariniflexile species, most of which are associated with marine habitats. The genomic features of TRM1-10 and other strains in the species may allow the taxon to adapt to the soil and rhizosphere, compete effectively with the resident soil microbiota, and contribute to plant health. |
| title | Polyphasic and comparative genomic characterization of a novel Mariniflexile species in the rhizosphere microbiome of tomato resistant to bacterial wilt. |
| topic | Solanum lycopersicum Rhizosphere Phylogeny Plant Diseases RNA, Ribosomal, 16S Microbiota Genome, Bacterial Ralstonia solanacearum Soil Microbiology Genomics Disease Resistance |
| url | https://pubmed.ncbi.nlm.nih.gov/41102233/ |