Upregulation of Canthaxanthin Biosynthesis by PH1 from Hot-Spring Origin via Sustainable Fermentation Strategy in Laboratory-Scale Bioreactor.

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Main Authors:	Inyoo, Anuttree, Pinmanee, Phitsanu, Thongkred, Paweena, Wongratpanya, Kanok, Kanokrung, Amonrat, Watanadilok, Rawiwan, Pekkoh, Jeeraporn, Pumas, Chayakorn, Sattayawat, Pachara, Bovonsombut, Sakunnee, Pathom-Aree, Wasu, Nimchua, Thidarat, Chitov, Thararat
Format:	Artículo científico
Language:	en
Published:	Biology 2025
Online Access:	Acceder al recurso
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author	Inyoo, Anuttree Pinmanee, Phitsanu Thongkred, Paweena Wongratpanya, Kanok Kanokrung, Amonrat Watanadilok, Rawiwan Pekkoh, Jeeraporn Pumas, Chayakorn Sattayawat, Pachara Bovonsombut, Sakunnee Pathom-Aree, Wasu Nimchua, Thidarat Chitov, Thararat
author_facet	Inyoo, Anuttree Pinmanee, Phitsanu Thongkred, Paweena Wongratpanya, Kanok Kanokrung, Amonrat Watanadilok, Rawiwan Pekkoh, Jeeraporn Pumas, Chayakorn Sattayawat, Pachara Bovonsombut, Sakunnee Pathom-Aree, Wasu Nimchua, Thidarat Chitov, Thararat Inyoo, Anuttree Pinmanee, Phitsanu Thongkred, Paweena Wongratpanya, Kanok Kanokrung, Amonrat Watanadilok, Rawiwan Pekkoh, Jeeraporn Pumas, Chayakorn Sattayawat, Pachara Bovonsombut, Sakunnee Pathom-Aree, Wasu Nimchua, Thidarat Chitov, Thararat
collection	PubMed - marine biology
contents	Upregulation of Canthaxanthin Biosynthesis by PH1 from Hot-Spring Origin via Sustainable Fermentation Strategy in Laboratory-Scale Bioreactor. Inyoo, Anuttree Pinmanee, Phitsanu Thongkred, Paweena Wongratpanya, Kanok Kanokrung, Amonrat Watanadilok, Rawiwan Pekkoh, Jeeraporn Pumas, Chayakorn Sattayawat, Pachara Bovonsombut, Sakunnee Pathom-Aree, Wasu Nimchua, Thidarat Chitov, Thararat Canthaxanthin is a significant carotenoid that is synthesized by specific microorganisms. It has multiple functions and has been utilized in food and feed supply chains. This research focused on improving canthaxanthin production by PH1, an orange-pigmented bacterium isolated from hot springs. Canthaxanthin production was optimized in flask-scale cultures by varying the pH, temperature, nutritional sources, aeration rates, and agitation techniques. Flask culture cultivation indicated that canthaxanthin production by this strain was influenced by pH stress mechanisms, resulting in the establishment of a two-stage pH control (pH-shift) technique to enhance cell mass and pigment production. The optimum flask conditions were refined for application in a 1 L bioreactor. An optimized cultivation procedure was established utilizing a Polypeptone Sucrose Yeast Extract (PPSYE) medium, with a pH transition from 7 to 11, incubation at 40 °C, agitation at 250 rpm, and aeration at 2 vvm for 48 h. This process resulted in a 3.12-fold increase in total carotenoid content and a 1.61-fold increase in canthaxanthin production, achieving 0.84 ± 0.06 mg/L compared to pre-optimized flask cultures in TSYEB medium (pH 7 at 37 °C, 72 h). Purified canthaxanthin from PH1 exhibited antioxidant activity in the ABTS assay.
format	Artículo científico
id	pubmed_41154737
institution	PubMed
language	en
publishDate	2025
publisher	Biology
record_format	pubmed
spellingShingle	Upregulation of Canthaxanthin Biosynthesis by PH1 from Hot-Spring Origin via Sustainable Fermentation Strategy in Laboratory-Scale Bioreactor. Inyoo, Anuttree Pinmanee, Phitsanu Thongkred, Paweena Wongratpanya, Kanok Kanokrung, Amonrat Watanadilok, Rawiwan Pekkoh, Jeeraporn Pumas, Chayakorn Sattayawat, Pachara Bovonsombut, Sakunnee Pathom-Aree, Wasu Nimchua, Thidarat Chitov, Thararat Upregulation of Canthaxanthin Biosynthesis by PH1 from Hot-Spring Origin via Sustainable Fermentation Strategy in Laboratory-Scale Bioreactor. Inyoo, Anuttree Pinmanee, Phitsanu Thongkred, Paweena Wongratpanya, Kanok Kanokrung, Amonrat Watanadilok, Rawiwan Pekkoh, Jeeraporn Pumas, Chayakorn Sattayawat, Pachara Bovonsombut, Sakunnee Pathom-Aree, Wasu Nimchua, Thidarat Chitov, Thararat Canthaxanthin is a significant carotenoid that is synthesized by specific microorganisms. It has multiple functions and has been utilized in food and feed supply chains. This research focused on improving canthaxanthin production by PH1, an orange-pigmented bacterium isolated from hot springs. Canthaxanthin production was optimized in flask-scale cultures by varying the pH, temperature, nutritional sources, aeration rates, and agitation techniques. Flask culture cultivation indicated that canthaxanthin production by this strain was influenced by pH stress mechanisms, resulting in the establishment of a two-stage pH control (pH-shift) technique to enhance cell mass and pigment production. The optimum flask conditions were refined for application in a 1 L bioreactor. An optimized cultivation procedure was established utilizing a Polypeptone Sucrose Yeast Extract (PPSYE) medium, with a pH transition from 7 to 11, incubation at 40 °C, agitation at 250 rpm, and aeration at 2 vvm for 48 h. This process resulted in a 3.12-fold increase in total carotenoid content and a 1.61-fold increase in canthaxanthin production, achieving 0.84 ± 0.06 mg/L compared to pre-optimized flask cultures in TSYEB medium (pH 7 at 37 °C, 72 h). Purified canthaxanthin from PH1 exhibited antioxidant activity in the ABTS assay.
title	Upregulation of Canthaxanthin Biosynthesis by PH1 from Hot-Spring Origin via Sustainable Fermentation Strategy in Laboratory-Scale Bioreactor.
url	https://pubmed.ncbi.nlm.nih.gov/41154737/

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