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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Biology
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41154866/ |
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| _version_ | 1868266136424939520 |
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| author | Liu, Zeyu Li, Weigang Wang, Fengchi Lu, Wei Yang, Fan Zhang, Qingke Cheng, Jie |
| author_facet | Liu, Zeyu Li, Weigang Wang, Fengchi Lu, Wei Yang, Fan Zhang, Qingke Cheng, Jie Liu, Zeyu Li, Weigang Wang, Fengchi Lu, Wei Yang, Fan Zhang, Qingke Cheng, Jie |
| collection | PubMed - marine biology |
| contents | Dynamic Regulation of Gonadal Transposons and Pseudogenes via PIWI/piRNA Pathway in Gynogenetic Japanese Flounder (). Liu, Zeyu Li, Weigang Wang, Fengchi Lu, Wei Yang, Fan Zhang, Qingke Cheng, Jie PIWI-interacting RNAs (piRNAs) are small non-coding RNAs that interact with PIWI proteins and play essential roles in genome stability, gonadal development, and gametogenesis in animals. The Japanese flounder () is an important marine culture teleost in North Asia, showing pronounced sexual size dimorphism, where gynogenetic induction of all-female cohorts can markedly enhance production. However, the PIWI/piRNA pathway in gynogenetic diploid , which often exhibit gonadal dysgenesis, poor gamete quality, and low fertilization rates, remains poorly understood. In this study, RNA-seq and small RNA-seq data from 11 tissues and 6 developmental stages of common , as well as the gonads of gynogenetic were analyzed to characterize the PIWI/piRNA pathway and its roles in transposon and gene regulation within the germline. The results showed that PIWI/piRNA genes were predominantly expressed in gonads and early embryogenesis in common , with the highest expression in testis. Clustered piRNAs were identified in the testis and early embryos of common , which targeted multiple transposon and gene families. Intriguingly, gynogenetic gonads harbored abundant clustered piRNAs not only in the testes but also in the ovaries, both targeting similar transposon families as that in common . Notably, the DNA transposon family and genes were the most heavily targeted by piRNAs in gynogenetic , with testis-biased expression. Expanded genes were identified in , overlapping with piRNA clusters, and the test in testes revealed that the expanded genes may be pseudogenes as a piRNA cluster reference to generate piRNAs regulating the conventional members. These unique features of the PIWI/piRNA pathway in gynogenetic diploid may underline their impaired reproductive ability, and have important theoretical and practical implications for teleost gynogenetic breeding. |
| format | Artículo científico |
| id | pubmed_41154866 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Biology |
| record_format | pubmed |
| spellingShingle | Dynamic Regulation of Gonadal Transposons and Pseudogenes via PIWI/piRNA Pathway in Gynogenetic Japanese Flounder (). Liu, Zeyu Li, Weigang Wang, Fengchi Lu, Wei Yang, Fan Zhang, Qingke Cheng, Jie Dynamic Regulation of Gonadal Transposons and Pseudogenes via PIWI/piRNA Pathway in Gynogenetic Japanese Flounder (). Liu, Zeyu Li, Weigang Wang, Fengchi Lu, Wei Yang, Fan Zhang, Qingke Cheng, Jie PIWI-interacting RNAs (piRNAs) are small non-coding RNAs that interact with PIWI proteins and play essential roles in genome stability, gonadal development, and gametogenesis in animals. The Japanese flounder () is an important marine culture teleost in North Asia, showing pronounced sexual size dimorphism, where gynogenetic induction of all-female cohorts can markedly enhance production. However, the PIWI/piRNA pathway in gynogenetic diploid , which often exhibit gonadal dysgenesis, poor gamete quality, and low fertilization rates, remains poorly understood. In this study, RNA-seq and small RNA-seq data from 11 tissues and 6 developmental stages of common , as well as the gonads of gynogenetic were analyzed to characterize the PIWI/piRNA pathway and its roles in transposon and gene regulation within the germline. The results showed that PIWI/piRNA genes were predominantly expressed in gonads and early embryogenesis in common , with the highest expression in testis. Clustered piRNAs were identified in the testis and early embryos of common , which targeted multiple transposon and gene families. Intriguingly, gynogenetic gonads harbored abundant clustered piRNAs not only in the testes but also in the ovaries, both targeting similar transposon families as that in common . Notably, the DNA transposon family and genes were the most heavily targeted by piRNAs in gynogenetic , with testis-biased expression. Expanded genes were identified in , overlapping with piRNA clusters, and the test in testes revealed that the expanded genes may be pseudogenes as a piRNA cluster reference to generate piRNAs regulating the conventional members. These unique features of the PIWI/piRNA pathway in gynogenetic diploid may underline their impaired reproductive ability, and have important theoretical and practical implications for teleost gynogenetic breeding. |
| title | Dynamic Regulation of Gonadal Transposons and Pseudogenes via PIWI/piRNA Pathway in Gynogenetic Japanese Flounder (). |
| url | https://pubmed.ncbi.nlm.nih.gov/41154866/ |