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| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Microorganisms
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41156872/ |
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Table of Contents:
- Unlocking the Potential of __: A Newly Isolated Bacteriophage Against . Plata Suarez, Laura Marcela Del Valle Balbuena, Salvador Becerra Mejía, Isamar Leticia Loera Piedra, Alejandra Aidee Domínguez Espinoza, Cristina Ángeles González, Arantxa Monserrat Contreras Rodríguez, Araceli Aquino Andrade, Alejandra Martínez Díaz, Sergio Francisco Aguilera Arreola, Ma Guadalupe Phage therapy has emerged as a promising alternative for combating infections caused by drug-resistant pathogens. Among these, remains a significant public health concern due to its persistence in clinical settings and frequent involvement in healthcare-associated infections (HAIs). In this study, we report the characterization of the lytic bacteriophage , isolated from urban sewage using strain IIH-74.4 as the host. Transmission electron microscopy revealed morphological features consistent with the phages formerly classified within the family. The phage exhibited high thermal and pH stability, remaining viable up to 70 °C and within a pH range of 4-11. It displayed a latent period of 20 min and a burst size of 72 PFU/cell. Notably, exhibited a narrow host range, lysing only the strain used for their isolation. Genomic analysis revealed a 41.2 kb double-stranded DNA genome devoid of known virulence or antibiotic resistance genes. Phylogenomic analysis classified the phage within the genus (Caudoviricetes), suggesting it represents a newly isolated bacteriophage species. Functional annotation identified genes related to DNA replication, host interaction, and bacterial lysis, including endolysins and holins with putative antimicrobial properties. Long-term stability assays demonstrated that tryptic soy broth (TSB) with CaCl/MgCl at 4 °C maintained viability for at least 90 days. Collectively, these findings support the potential of as a potential candidate for the development of phage-based therapies targeting .