Carbon-monoxide-driven bioethanol production operates through a tungsten-dependent catalyst.
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| Autores principales: | , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Nature chemical biology
2026
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| _version_ | 1868266132957298688 |
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| author | Lemaire, Olivier N Belhamri, Mélissa Shevchenko, Anna Wagner, Tristan |
| author_facet | Lemaire, Olivier N Belhamri, Mélissa Shevchenko, Anna Wagner, Tristan Lemaire, Olivier N Belhamri, Mélissa Shevchenko, Anna Wagner, Tristan |
| collection | PubMed - marine biology |
| contents | Carbon-monoxide-driven bioethanol production operates through a tungsten-dependent catalyst. Lemaire, Olivier N Belhamri, Mélissa Shevchenko, Anna Wagner, Tristan Tungsten Ethanol Carbon Monoxide Clostridium Biofuels Aldehyde Oxidoreductases Oxidation-Reduction Catalysis Multienzyme Complexes Ferredoxins Models, Molecular Microbial alcohol production from waste gases is a game changer for sustainable carbon cycling and remediation. While the biotechnological process using Clostridium autoethanogenum to transform syngas (H, CO and CO) is blooming, scientific debates remain on the ethanol biosynthesis pathway. Here, we experimentally validated that ethanol production is initiated through a tungsten-dependent aldehyde:ferredoxin oxidoreductase (AFOR), which reduces acetate to acetaldehyde. The reaction, thermodynamically unfavorable under standard conditions, has been considered by many as unsuitable in vivo but is rather approved by metabolic modeling. To answer this riddle, we demonstrated that the thermodynamic coupling of CO oxidation and ethanol synthesis allows acetate reduction. The experiments, performed with native CO dehydrogenase and AFOR, highlighted the key role of ferredoxin in stimulating the activity of both metalloenzymes and electron shuttling. The crystal structure of holo AFOR, refined to 1.59-Å resolution, and its biochemical characterization provide new insights into the cofactor chemistry and the specificities of this enzyme, fundamental to sustainable biofuel production. |
| format | Artículo científico |
| id | pubmed_41162695 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Nature chemical biology |
| record_format | pubmed |
| spellingShingle | Carbon-monoxide-driven bioethanol production operates through a tungsten-dependent catalyst. Lemaire, Olivier N Belhamri, Mélissa Shevchenko, Anna Wagner, Tristan Tungsten Ethanol Carbon Monoxide Clostridium Biofuels Aldehyde Oxidoreductases Oxidation-Reduction Catalysis Multienzyme Complexes Ferredoxins Models, Molecular Carbon-monoxide-driven bioethanol production operates through a tungsten-dependent catalyst. Lemaire, Olivier N Belhamri, Mélissa Shevchenko, Anna Wagner, Tristan Tungsten Ethanol Carbon Monoxide Clostridium Biofuels Aldehyde Oxidoreductases Oxidation-Reduction Catalysis Multienzyme Complexes Ferredoxins Models, Molecular Microbial alcohol production from waste gases is a game changer for sustainable carbon cycling and remediation. While the biotechnological process using Clostridium autoethanogenum to transform syngas (H, CO and CO) is blooming, scientific debates remain on the ethanol biosynthesis pathway. Here, we experimentally validated that ethanol production is initiated through a tungsten-dependent aldehyde:ferredoxin oxidoreductase (AFOR), which reduces acetate to acetaldehyde. The reaction, thermodynamically unfavorable under standard conditions, has been considered by many as unsuitable in vivo but is rather approved by metabolic modeling. To answer this riddle, we demonstrated that the thermodynamic coupling of CO oxidation and ethanol synthesis allows acetate reduction. The experiments, performed with native CO dehydrogenase and AFOR, highlighted the key role of ferredoxin in stimulating the activity of both metalloenzymes and electron shuttling. The crystal structure of holo AFOR, refined to 1.59-Å resolution, and its biochemical characterization provide new insights into the cofactor chemistry and the specificities of this enzyme, fundamental to sustainable biofuel production. |
| title | Carbon-monoxide-driven bioethanol production operates through a tungsten-dependent catalyst. |
| topic | Tungsten Ethanol Carbon Monoxide Clostridium Biofuels Aldehyde Oxidoreductases Oxidation-Reduction Catalysis Multienzyme Complexes Ferredoxins Models, Molecular |
| url | https://pubmed.ncbi.nlm.nih.gov/41162695/ |