DNA/RNA hybrid profiling in autistic patients: A focus on mRNA and non-coding RNA variations.
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| Auteurs principaux: | , , , , , , , |
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| Format: | Artículo científico |
| Langue: | en |
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PloS one
2025
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| _version_ | 1868266132927938560 |
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| author | Kianmehr, Leila MokhtarZadeh, Kasra Yilmaz, Zeynep Darzi Ramandi, Hadi Mehmetbeyoglu Duman, Ecmel Funda Sener, Elif Taheri, Serpil Rassoulzadegan, Minoo |
| author_facet | Kianmehr, Leila MokhtarZadeh, Kasra Yilmaz, Zeynep Darzi Ramandi, Hadi Mehmetbeyoglu Duman, Ecmel Funda Sener, Elif Taheri, Serpil Rassoulzadegan, Minoo Kianmehr, Leila MokhtarZadeh, Kasra Yilmaz, Zeynep Darzi Ramandi, Hadi Mehmetbeyoglu Duman, Ecmel Funda Sener, Elif Taheri, Serpil Rassoulzadegan, Minoo |
| collection | PubMed - marine biology |
| contents | DNA/RNA hybrid profiling in autistic patients: A focus on mRNA and non-coding RNA variations. Kianmehr, Leila MokhtarZadeh, Kasra Yilmaz, Zeynep Darzi Ramandi, Hadi Mehmetbeyoglu Duman, Ecmel Funda Sener, Elif Taheri, Serpil Rassoulzadegan, Minoo Humans RNA, Messenger Male Female Gene Expression Profiling Autism Spectrum Disorder Child RNA, Untranslated Case-Control Studies RNA, Long Noncoding Autistic Disorder DNA Transcriptome Nucleic Acid Hybridization Autism spectrum disorder (ASD) is a set of genetically heterogenous neurodevelopmental disorders characterized by core symptoms including impaired social interaction, communication deficits, and restricted or stereotyped behaviors. While a significant number of cases are not explained by Mendelian inheritance, there is growing evidence for implication of non-coding RNAs (ncRNAs) in the development and inheritance of ASD. Transcriptional studies often face challenges due to patient-specific variations in gene expression and technical differences in preserving RNA integrity. We propose that isolating RNA from DNA/RNA hybrids provides a robust method to reliably capture transcriptional information. We performed a whole transcriptome analysis on blood samples from ASD patients and healthy controls to investigate transcripts associated with DNA/RNA hybrids. We identified 278,300 novel transcripts across 68,487 DNA/RNA hybrid loci, with significant enrichment in exonic and intronic regions. The novel long non-coding RNAs (lncRNAs) we found showed higher expression levels compared to known transcripts. Differential expression analysis revealed 301 significantly upregulated and 401 downregulated known transcripts in ASD samples compared to controls (|log2-fold change| > 1 and adjusted p-value |
| format | Artículo científico |
| id | pubmed_41183059 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | PloS one |
| record_format | pubmed |
| spellingShingle | DNA/RNA hybrid profiling in autistic patients: A focus on mRNA and non-coding RNA variations. Kianmehr, Leila MokhtarZadeh, Kasra Yilmaz, Zeynep Darzi Ramandi, Hadi Mehmetbeyoglu Duman, Ecmel Funda Sener, Elif Taheri, Serpil Rassoulzadegan, Minoo Humans RNA, Messenger Male Female Gene Expression Profiling Autism Spectrum Disorder Child RNA, Untranslated Case-Control Studies RNA, Long Noncoding Autistic Disorder DNA Transcriptome Nucleic Acid Hybridization DNA/RNA hybrid profiling in autistic patients: A focus on mRNA and non-coding RNA variations. Kianmehr, Leila MokhtarZadeh, Kasra Yilmaz, Zeynep Darzi Ramandi, Hadi Mehmetbeyoglu Duman, Ecmel Funda Sener, Elif Taheri, Serpil Rassoulzadegan, Minoo Humans RNA, Messenger Male Female Gene Expression Profiling Autism Spectrum Disorder Child RNA, Untranslated Case-Control Studies RNA, Long Noncoding Autistic Disorder DNA Transcriptome Nucleic Acid Hybridization Autism spectrum disorder (ASD) is a set of genetically heterogenous neurodevelopmental disorders characterized by core symptoms including impaired social interaction, communication deficits, and restricted or stereotyped behaviors. While a significant number of cases are not explained by Mendelian inheritance, there is growing evidence for implication of non-coding RNAs (ncRNAs) in the development and inheritance of ASD. Transcriptional studies often face challenges due to patient-specific variations in gene expression and technical differences in preserving RNA integrity. We propose that isolating RNA from DNA/RNA hybrids provides a robust method to reliably capture transcriptional information. We performed a whole transcriptome analysis on blood samples from ASD patients and healthy controls to investigate transcripts associated with DNA/RNA hybrids. We identified 278,300 novel transcripts across 68,487 DNA/RNA hybrid loci, with significant enrichment in exonic and intronic regions. The novel long non-coding RNAs (lncRNAs) we found showed higher expression levels compared to known transcripts. Differential expression analysis revealed 301 significantly upregulated and 401 downregulated known transcripts in ASD samples compared to controls (|log2-fold change| > 1 and adjusted p-value |
| title | DNA/RNA hybrid profiling in autistic patients: A focus on mRNA and non-coding RNA variations. |
| topic | Humans RNA, Messenger Male Female Gene Expression Profiling Autism Spectrum Disorder Child RNA, Untranslated Case-Control Studies RNA, Long Noncoding Autistic Disorder DNA Transcriptome Nucleic Acid Hybridization |
| url | https://pubmed.ncbi.nlm.nih.gov/41183059/ |