Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Biosensors & bioelectronics
2026
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41183413/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266132926889984 |
|---|---|
| author | Chen, Huiyou Zeng, Zhixi Wei, Yangdao Huang, Hui Negahdary, Masoud Han, Xiaosheng Lin, Yezhou He, Linwen Song, Fengge Wan, Yi |
| author_facet | Chen, Huiyou Zeng, Zhixi Wei, Yangdao Huang, Hui Negahdary, Masoud Han, Xiaosheng Lin, Yezhou He, Linwen Song, Fengge Wan, Yi Chen, Huiyou Zeng, Zhixi Wei, Yangdao Huang, Hui Negahdary, Masoud Han, Xiaosheng Lin, Yezhou He, Linwen Song, Fengge Wan, Yi |
| collection | PubMed - marine biology |
| contents | Dynamic biosensing enables amplifier-collateral-cleavage enhancement for pathogen diagnostic. Chen, Huiyou Zeng, Zhixi Wei, Yangdao Huang, Hui Negahdary, Masoud Han, Xiaosheng Lin, Yezhou He, Linwen Song, Fengge Wan, Yi Biosensing Techniques CRISPR-Cas Systems Humans DNA While CRISPR-Cas system and dynamic DNA nanotechnology have been extensively applied to mainstream biomedical domains, including gene editing, biochemical analysis, and molecular imaging-current approaches remain constrained by limitations in addressing increasingly nuanced and specialized experimental scenarios. Here, we report that CRISPR-CasΦ possesses a unique collateral-cleavage blockade characteristic: CasΦ is unable to recognize the "TTN" sequence in the loop region at the 3' end of stem-loop DNA, resulting in the blockade of collateral-cleavage activity. Leveraging this discovery, we successfully designed and customized two back-end signal amplifiers for biosensing by integrating dynamic DNA sensing studies of CasΦ. Based on these two specialized stem-loop amplifiers, we further developed the Amplifier-collateral-cleavage enhancement (ACE) method, achieving exponential signal amplification. Clinical validation using 112 urine samples demonstrated that ACE exhibits 98.8 % sensitivity and 90 % specificity. These findings highlight the potential of CasΦ dynamic sensing and establish a bridge for future integration of dynamic DNA technology and CRISPR systems. |
| format | Artículo científico |
| id | pubmed_41183413 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Biosensors & bioelectronics |
| record_format | pubmed |
| spellingShingle | Dynamic biosensing enables amplifier-collateral-cleavage enhancement for pathogen diagnostic. Chen, Huiyou Zeng, Zhixi Wei, Yangdao Huang, Hui Negahdary, Masoud Han, Xiaosheng Lin, Yezhou He, Linwen Song, Fengge Wan, Yi Biosensing Techniques CRISPR-Cas Systems Humans DNA Dynamic biosensing enables amplifier-collateral-cleavage enhancement for pathogen diagnostic. Chen, Huiyou Zeng, Zhixi Wei, Yangdao Huang, Hui Negahdary, Masoud Han, Xiaosheng Lin, Yezhou He, Linwen Song, Fengge Wan, Yi Biosensing Techniques CRISPR-Cas Systems Humans DNA While CRISPR-Cas system and dynamic DNA nanotechnology have been extensively applied to mainstream biomedical domains, including gene editing, biochemical analysis, and molecular imaging-current approaches remain constrained by limitations in addressing increasingly nuanced and specialized experimental scenarios. Here, we report that CRISPR-CasΦ possesses a unique collateral-cleavage blockade characteristic: CasΦ is unable to recognize the "TTN" sequence in the loop region at the 3' end of stem-loop DNA, resulting in the blockade of collateral-cleavage activity. Leveraging this discovery, we successfully designed and customized two back-end signal amplifiers for biosensing by integrating dynamic DNA sensing studies of CasΦ. Based on these two specialized stem-loop amplifiers, we further developed the Amplifier-collateral-cleavage enhancement (ACE) method, achieving exponential signal amplification. Clinical validation using 112 urine samples demonstrated that ACE exhibits 98.8 % sensitivity and 90 % specificity. These findings highlight the potential of CasΦ dynamic sensing and establish a bridge for future integration of dynamic DNA technology and CRISPR systems. |
| title | Dynamic biosensing enables amplifier-collateral-cleavage enhancement for pathogen diagnostic. |
| topic | Biosensing Techniques CRISPR-Cas Systems Humans DNA |
| url | https://pubmed.ncbi.nlm.nih.gov/41183413/ |