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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Applied biochemistry and biotechnology
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41201686/ |
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Table of Contents:
- Phycobiliproteins of Marine Red Alga, Kappaphycus Alvarezii Possess Potent Antioxidant and Metal Chelating Properties, and Inhibit Hepatocellular Carcinoma (HepG2) Cell Growth. Mahendran, Gayathridevi Stephen, Nimish Mol Manivel, Mangaiyarkarasi Maradagi, Tehreem Nagarajan, Muralidharan Natarajan, Jayakumar Ponesakki, Ganesan Rhodophyta Humans Antioxidants Hep G2 Cells Chelating Agents Phycobiliproteins Liver Neoplasms Carcinoma, Hepatocellular Cell Proliferation Apoptosis Metals Edible Seaweeds Kappaphycus alvarezii is one of the economically important red alga, harvested primarily for the production of hydrocolloids. This study aimed to identify an effective extraction method for the isolation of phycobiliproteins (PBPs) from K. alvarezii and evaluate its biofunctional properties. Organic solvents, inorganic acids, organic acids, phosphate buffer saline (PBS), distilled water, and freeze-thawing were used to identify an effective extraction method. The identification of PBPs was carried out by UV-Vis spectrophotometry, FTIR spectroscopy, and SDS-PAGE analysis. The antioxidant potentials of PBPs was examined by DPPH and ABTS radical scavenging assays. The metal chelating efficiency was determined using divalent, monovalent, and trivalent metal ions. The WST-1 assay and DAPI staining were performed to examine HepG2 cell viability and apoptosis, respectively. Among the extraction methods, higher content of phycoeryhrin was recorded in phosphate buffer saline. The results showed that extraction using PBS exhibits highest content of phycoerythrin. The PBPs fraction contains phycoerythrin as a major pigment molecule. The isolated PBPs exhibited increased DPPH and ABTS radical scavenging activity in a concentration-dependent manner. It has possible quenching ability of Co and Cu. The isolated PBPs inhibited HepG2 cell growth in a dose-dependent manner and induced nuclear fragmentation. The data emphasize that K. alvarezii could be utilized for the extraction of phycoerythrin which has antioxidant, metal chelation, and anticancer properties.