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| Main Authors: | , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Nature communications
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41219190/ |
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| author | Chung, Dudley Brask, Nikolaj Matar, Sari Gallot-Lavallée, Lucie Pringle, Eric S Duguay, Brett A Blais, Cédric Latimer, Jessica Haro, Ronie Slamovits, Claudio H Leyland, Ben Rest, Joshua S Collier, Jackie L McCormick, Craig Archibald, John M |
| author_facet | Chung, Dudley Brask, Nikolaj Matar, Sari Gallot-Lavallée, Lucie Pringle, Eric S Duguay, Brett A Blais, Cédric Latimer, Jessica Haro, Ronie Slamovits, Claudio H Leyland, Ben Rest, Joshua S Collier, Jackie L McCormick, Craig Archibald, John M Chung, Dudley Brask, Nikolaj Matar, Sari Gallot-Lavallée, Lucie Pringle, Eric S Duguay, Brett A Blais, Cédric Latimer, Jessica Haro, Ronie Slamovits, Claudio H Leyland, Ben Rest, Joshua S Collier, Jackie L McCormick, Craig Archibald, John M |
| collection | PubMed - marine biology |
| contents | Persistent mirusvirus infection in the marine protist Aurantiochytrium. Chung, Dudley Brask, Nikolaj Matar, Sari Gallot-Lavallée, Lucie Pringle, Eric S Duguay, Brett A Blais, Cédric Latimer, Jessica Haro, Ronie Slamovits, Claudio H Leyland, Ben Rest, Joshua S Collier, Jackie L McCormick, Craig Archibald, John M Stramenopiles DNA Viruses Genome, Viral Virion DNA, Viral Viral Proteins Plasmids Proteomics Mirusviruses are abundant and broadly distributed double-stranded (ds) DNA viruses recently discovered in marine metagenomic data. Their host range and ecological impact are unclear. The protist Aurantiochytrium limacinum possesses two mirusvirus-like genomic elements, one a circular episome (AurliV-1) and the other (AurliV-2) a chromosomal integrant. Here we show that genes in both genomes are expressed and viral particles containing mainly AurliV-1 DNA are produced under starvation conditions and when cells are cultured in standard growth medium. We detected viral particles of ~140 nm in the nucleus, in cytoplasmic vesicles, between the plasma membrane and cell wall, and in the extracellular environment. Of 67 AurliV-1-encoded proteins detected using proteomics, 45 are enriched under starvation conditions, including the structurally important major capsid and triplex proteins. Our results establish Aurantiochytrium as a model system for elucidating mirusvirus-host interactions and demonstrate persistent viral infection in a microbial eukaryote. |
| format | Artículo científico |
| id | pubmed_41219190 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Nature communications |
| record_format | pubmed |
| spellingShingle | Persistent mirusvirus infection in the marine protist Aurantiochytrium. Chung, Dudley Brask, Nikolaj Matar, Sari Gallot-Lavallée, Lucie Pringle, Eric S Duguay, Brett A Blais, Cédric Latimer, Jessica Haro, Ronie Slamovits, Claudio H Leyland, Ben Rest, Joshua S Collier, Jackie L McCormick, Craig Archibald, John M Stramenopiles DNA Viruses Genome, Viral Virion DNA, Viral Viral Proteins Plasmids Proteomics Persistent mirusvirus infection in the marine protist Aurantiochytrium. Chung, Dudley Brask, Nikolaj Matar, Sari Gallot-Lavallée, Lucie Pringle, Eric S Duguay, Brett A Blais, Cédric Latimer, Jessica Haro, Ronie Slamovits, Claudio H Leyland, Ben Rest, Joshua S Collier, Jackie L McCormick, Craig Archibald, John M Stramenopiles DNA Viruses Genome, Viral Virion DNA, Viral Viral Proteins Plasmids Proteomics Mirusviruses are abundant and broadly distributed double-stranded (ds) DNA viruses recently discovered in marine metagenomic data. Their host range and ecological impact are unclear. The protist Aurantiochytrium limacinum possesses two mirusvirus-like genomic elements, one a circular episome (AurliV-1) and the other (AurliV-2) a chromosomal integrant. Here we show that genes in both genomes are expressed and viral particles containing mainly AurliV-1 DNA are produced under starvation conditions and when cells are cultured in standard growth medium. We detected viral particles of ~140 nm in the nucleus, in cytoplasmic vesicles, between the plasma membrane and cell wall, and in the extracellular environment. Of 67 AurliV-1-encoded proteins detected using proteomics, 45 are enriched under starvation conditions, including the structurally important major capsid and triplex proteins. Our results establish Aurantiochytrium as a model system for elucidating mirusvirus-host interactions and demonstrate persistent viral infection in a microbial eukaryote. |
| title | Persistent mirusvirus infection in the marine protist Aurantiochytrium. |
| topic | Stramenopiles DNA Viruses Genome, Viral Virion DNA, Viral Viral Proteins Plasmids Proteomics |
| url | https://pubmed.ncbi.nlm.nih.gov/41219190/ |