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Auteurs principaux: Chen, Renwei, Nordheim-Maestas, Caitlin L, Briggs, Cheryl J
Format: Artículo científico
Langue:en
Publié: ISME communications 2025
Accès en ligne:https://pubmed.ncbi.nlm.nih.gov/41221506/
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author Chen, Renwei
Nordheim-Maestas, Caitlin L
Briggs, Cheryl J
author_facet Chen, Renwei
Nordheim-Maestas, Caitlin L
Briggs, Cheryl J
Chen, Renwei
Nordheim-Maestas, Caitlin L
Briggs, Cheryl J
collection PubMed - marine biology
contents Double-edged effects of aquatic environmental biofilms on growth and inhibition. Chen, Renwei Nordheim-Maestas, Caitlin L Briggs, Cheryl J (Bd) is an aquatic chytrid fungus that infects amphibians and has the potential to remain viable outside of hosts. However, the role of aquatic microbiota in influencing Bd growth and survival remains insufficiently understood. In this study, we demonstrated that in the absence of amphibian hosts, aquatic environmental (AE) biofilms supported the development of Bd, allowing it to complete its life cycle for a short period; whereas aquatic planktonic microorganisms did not. However, exposure of Bd zoospores to AE biofilms or planktonic microorganisms resulted in a significant reduction in Bd DNA within a week. These results suggest a dual role of aquatic biofilms in both supporting Bd growth and inhibiting it simultaneously. Moreover, Bd monolayers, composed mainly of zoosporangia, rapidly declined when exposed to AE planktonic microorganisms. Laboratory-formulated nutrients further enhanced the Bd-inhibitory effect of AE microbiota, suggesting that competition for shared nutrients plays a role in this interaction. This study advances our understanding of the complex interactions between Bd and aquatic microbial communities, underscores the ecological significance of biofilm-associated environments, and supports the potential of microbiota-informed interventions for controlling chytridiomycosis in amphibians.
format Artículo científico
id pubmed_41221506
institution PubMed
language en
publishDate 2025
publisher ISME communications
record_format pubmed
spellingShingle Double-edged effects of aquatic environmental biofilms on growth and inhibition.
Chen, Renwei
Nordheim-Maestas, Caitlin L
Briggs, Cheryl J
Double-edged effects of aquatic environmental biofilms on growth and inhibition. Chen, Renwei Nordheim-Maestas, Caitlin L Briggs, Cheryl J (Bd) is an aquatic chytrid fungus that infects amphibians and has the potential to remain viable outside of hosts. However, the role of aquatic microbiota in influencing Bd growth and survival remains insufficiently understood. In this study, we demonstrated that in the absence of amphibian hosts, aquatic environmental (AE) biofilms supported the development of Bd, allowing it to complete its life cycle for a short period; whereas aquatic planktonic microorganisms did not. However, exposure of Bd zoospores to AE biofilms or planktonic microorganisms resulted in a significant reduction in Bd DNA within a week. These results suggest a dual role of aquatic biofilms in both supporting Bd growth and inhibiting it simultaneously. Moreover, Bd monolayers, composed mainly of zoosporangia, rapidly declined when exposed to AE planktonic microorganisms. Laboratory-formulated nutrients further enhanced the Bd-inhibitory effect of AE microbiota, suggesting that competition for shared nutrients plays a role in this interaction. This study advances our understanding of the complex interactions between Bd and aquatic microbial communities, underscores the ecological significance of biofilm-associated environments, and supports the potential of microbiota-informed interventions for controlling chytridiomycosis in amphibians.
title Double-edged effects of aquatic environmental biofilms on growth and inhibition.
url https://pubmed.ncbi.nlm.nih.gov/41221506/