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| Main Authors: | , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Proceedings of the National Academy of Sciences of the United States of America
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41231942/ |
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Table of Contents:
- protein kinase MAK phosphorylates FAP256/CEP104 and regulates axonemal microtubule assembly. Zhang, Yi Yang, Xinjia Pan, Junmin Axoneme Microtubules Phosphorylation Cilia Chlamydomonas reinhardtii Protein Kinases Chlamydomonas Microtubule-Associated Proteins The formation of cilia involves the assembly of axonemal microtubules, the structural core of the organelle, from tubulins that are delivered by intraflagellar transport and diffusion. Whether and how this process is controlled are not clear. Here, we show that MAK (CrMAK), related to mammalian ICK/MAK, regulates axonemal microtubule assembly by phosphorylating FAP256/CEP104, a microtubule plus-end tracking protein. CrMAK, primarily present in cilia, associates with the axoneme via its C-terminal tail, and is enriched at the ciliary tip during active ciliary assembly. It is constitutively active during this process and acts downstream of a ciliary length-regulatory complex composed of LF1, LF3, and LF2, a CCRK homologue. Loss of CrMAK or its kinase activity leads to aciliated cells. CrMAK interacts with and phosphorylates FAP256/CEP104 and dephosphorylation of FAP256/CEP104 impairs ciliary assembly. These findings reveal a molecular mechanism governing the assembly of axonemal microtubules.