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| Natura: | Artículo científico |
| Lingua: | en |
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Biochimica et biophysica acta. General subjects
2026
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| Soggetti: | |
| Accesso online: | https://pubmed.ncbi.nlm.nih.gov/41238195/ |
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| _version_ | 1868266127714418688 |
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| author | Bertsova, Yulia V Serebryakova, Marina V Baykov, Alexander A Bogachev, Alexander V |
| author_facet | Bertsova, Yulia V Serebryakova, Marina V Baykov, Alexander A Bogachev, Alexander V Bertsova, Yulia V Serebryakova, Marina V Baykov, Alexander A Bogachev, Alexander V |
| collection | PubMed - marine biology |
| contents | Histidine betaine trimethylammonia-lyase, enzyme coupled with terminal urocanate reductase in Shewanella woodyi grown anaerobically. Bertsova, Yulia V Serebryakova, Marina V Baykov, Alexander A Bogachev, Alexander V Shewanella Anaerobiosis Betaine Bacterial Proteins Ammonia-Lyases Oxidoreductases Histidine Multigene Family Methylamines Bacteria coping with oxygen deficiency can switch to alternative terminal electron acceptors, which can be normal metabolic intermediates or products of dedicated coupled reactions. In the latter case, the genes for the respective terminal reductase and coupling enzyme are expected to cluster in the genome. Here, we determined the roles of two uncharacterized periplasmic proteins encoded by the swoo_3912-swoo_3913 gene cluster in the facultatively anaerobic marine bacterium Shewanella woodyi. We confirmed the current database annotation of the former protein as "urocanate reductase" but identified the latter protein as a histidine betaine trimethylammonia-lyase (HBTL). HBTL converts histidine betaine into urocanate and trimethylamine and is remarkably specific for histidine betaine as substrate. HBTL requires Mg for activity and undergoes slow reversible inactivation at low Mg concentrations. HBTL activity was not evident in S. woodyi cells grown aerobically but was induced in cells grown anaerobically. Both histidine betaine and urocanate supported anaerobic S. woodyi growth and, hence, respiration. Similar gene clusters are found in many anaerobic bacteria, suggesting a wide occurrence of the anaerobic respiration pathway discovered in this work in the bacterial world. |
| format | Artículo científico |
| id | pubmed_41238195 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Biochimica et biophysica acta. General subjects |
| record_format | pubmed |
| spellingShingle | Histidine betaine trimethylammonia-lyase, enzyme coupled with terminal urocanate reductase in Shewanella woodyi grown anaerobically. Bertsova, Yulia V Serebryakova, Marina V Baykov, Alexander A Bogachev, Alexander V Shewanella Anaerobiosis Betaine Bacterial Proteins Ammonia-Lyases Oxidoreductases Histidine Multigene Family Methylamines Histidine betaine trimethylammonia-lyase, enzyme coupled with terminal urocanate reductase in Shewanella woodyi grown anaerobically. Bertsova, Yulia V Serebryakova, Marina V Baykov, Alexander A Bogachev, Alexander V Shewanella Anaerobiosis Betaine Bacterial Proteins Ammonia-Lyases Oxidoreductases Histidine Multigene Family Methylamines Bacteria coping with oxygen deficiency can switch to alternative terminal electron acceptors, which can be normal metabolic intermediates or products of dedicated coupled reactions. In the latter case, the genes for the respective terminal reductase and coupling enzyme are expected to cluster in the genome. Here, we determined the roles of two uncharacterized periplasmic proteins encoded by the swoo_3912-swoo_3913 gene cluster in the facultatively anaerobic marine bacterium Shewanella woodyi. We confirmed the current database annotation of the former protein as "urocanate reductase" but identified the latter protein as a histidine betaine trimethylammonia-lyase (HBTL). HBTL converts histidine betaine into urocanate and trimethylamine and is remarkably specific for histidine betaine as substrate. HBTL requires Mg for activity and undergoes slow reversible inactivation at low Mg concentrations. HBTL activity was not evident in S. woodyi cells grown aerobically but was induced in cells grown anaerobically. Both histidine betaine and urocanate supported anaerobic S. woodyi growth and, hence, respiration. Similar gene clusters are found in many anaerobic bacteria, suggesting a wide occurrence of the anaerobic respiration pathway discovered in this work in the bacterial world. |
| title | Histidine betaine trimethylammonia-lyase, enzyme coupled with terminal urocanate reductase in Shewanella woodyi grown anaerobically. |
| topic | Shewanella Anaerobiosis Betaine Bacterial Proteins Ammonia-Lyases Oxidoreductases Histidine Multigene Family Methylamines |
| url | https://pubmed.ncbi.nlm.nih.gov/41238195/ |