Saved in:
| Main Authors: | , , , , , , , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Harmful algae
2025
|
| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41241548/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- Ranking the cytotoxic potency of Ostreopsis cf. ovata, Ostreopsis cf. siamensis (O. sp. 9), and purified ovatoxins in human keratinocytes. Plessis, Loïc Lhaute, Korian Hervé, Fabienne Thimann, Madeleine Séchet, Véronique Carpentier, Liliane Moutinho, Ariana B Simões, Tiago Amorim, Ana Henry, Kevin Neves, Jorge L B Gorse, Léana Paradis, Margaux Brédif, Stéphanie Hess, Philipp Réveillon, Damien Acrylamides Keratinocytes Cell Death Polyketides Marine Toxins Ovatoxins (OVTXs), a family of marine polyketides structurally similar to palytoxin (PLTX), are suspected to cause respiratory symptoms and skin irritation among coastal users during blooms of the dinoflagellate Ostreopsis cf. ovata. To investigate their role in cutaneous toxicity, crude extracts from six O. cf. ovata strains (OVTX(+) and OVTX(-)) and three O. cf. siamensis (O. sp. 9) strains (OVTX(-)), along with six purified OVTX analogs, were tested for cytotoxicity (MTT assay) and cell death phenotypes (microscopy) in human keratinocyte models. Interestingly, cytotoxicity was driven by OVTX content, while OVTX(-) extracts were, on average, 150-fold and >1000-fold less potent for O. cf. siamensis and O. cf. ovata, respectively. Nevertheless, the latter two induced PLTX-like cell death, suggesting the presence of unidentified PLTX-like toxins. Purified OVTXs exhibited sub-nanomolar cytotoxicity, 3.5- to 12.5-fold lower than PLTX (IC = 0.036-0.642 nM vs. 0.011-0.049 nM), ranking in potency: PLTX > OVTX-d > OVTX-b > OVTX-a > OVTX-c > OVTX-e >>> OVTX-h (non-toxic, likely due to ring-opening). This study demonstrates the high toxic potency of OVTXs, close to that of PLTX, while providing a comprehensive cytotoxicity assessment of OVTX analogs, which could be useful for assessing the risks of human exposure.