Selenium nanoparticles conjugated with exopolysaccharides from marine hydrothermal vent crab-associated actinomycete Pseudonocardia alni SCSW01 and their antioxidant, antibacterial, and antibiofilm activities.
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| Autores principales: | , , , , , , |
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| Formato: | Artículo científico |
| Lenguaje: | en |
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Biomaterials advances
2026
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| _version_ | 1868266127702884354 |
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| author | Munir Ahamed, Johnthini Dayalan, Sandhiya Dahms, Hans-Uwe Khan, Muhammad Bilal Upadhyaya, Ujala Wu, Ming-Tsang Huang, Yeou-Lih |
| author_facet | Munir Ahamed, Johnthini Dayalan, Sandhiya Dahms, Hans-Uwe Khan, Muhammad Bilal Upadhyaya, Ujala Wu, Ming-Tsang Huang, Yeou-Lih Munir Ahamed, Johnthini Dayalan, Sandhiya Dahms, Hans-Uwe Khan, Muhammad Bilal Upadhyaya, Ujala Wu, Ming-Tsang Huang, Yeou-Lih |
| collection | PubMed - marine biology |
| contents | Selenium nanoparticles conjugated with exopolysaccharides from marine hydrothermal vent crab-associated actinomycete Pseudonocardia alni SCSW01 and their antioxidant, antibacterial, and antibiofilm activities. Munir Ahamed, Johnthini Dayalan, Sandhiya Dahms, Hans-Uwe Khan, Muhammad Bilal Upadhyaya, Ujala Wu, Ming-Tsang Huang, Yeou-Lih Biofilms Anti-Bacterial Agents Antioxidants Selenium Animals Staphylococcus aureus Nanoparticles Microbial Sensitivity Tests Brachyura Hydrothermal Vents Escherichia coli Staphylococcus epidermidis Polysaccharides, Bacterial Multidrug-resistant infections demand agents that combine antibacterial and antibiofilm activity. We synthesized selenium nanoparticles (SeNPs) and conjugated them with exopolysaccharides (EPS) from the shallow-water hydrothermal vent actinomycete Pseudonocardia alni SCSW01. Culture optimization increased EPS yield to 4.05 g/L. Physicochemical and microscopic analyses confirmed the formation of stable SeNPs-EPS. SeNPs exhibited a crystalline structure, whereas EPS was amorphous, and conjugation produced a negative surface potential (ζ ≈ -25 mV), indicating enhanced colloidal stability. Functionally, EPS exhibited the highest antioxidant capacity, whereas SeNPs-EPS demonstrated the most potent antibacterial activity. At 50 μg/mL concentration, SeNPs-EPS inhibited the growth of Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli; SeNPs or EPS alone were selectively active against S. epidermidis. Field emission scanning electron microscope (FESEM) images showed that SeNPs, SeNPs-EPS, and EPS caused substantial morphological damage in S. aureus. Confocal laser scanning microscopy images and antibiofilm assay results confirmed that SeNPs-EPS exhibited strong antibiofilm activity against S. aureus and E. coli. To our knowledge, this is the first report of EPS production, medium optimization, and SeNPs-EPS conjugation from Pseudonocardia alni SCSW01. Our study results indicate that SeNPs-EPS is a promising anti-biofilm coating candidate for medical devices. |
| format | Artículo científico |
| id | pubmed_41242058 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Biomaterials advances |
| record_format | pubmed |
| spellingShingle | Selenium nanoparticles conjugated with exopolysaccharides from marine hydrothermal vent crab-associated actinomycete Pseudonocardia alni SCSW01 and their antioxidant, antibacterial, and antibiofilm activities. Munir Ahamed, Johnthini Dayalan, Sandhiya Dahms, Hans-Uwe Khan, Muhammad Bilal Upadhyaya, Ujala Wu, Ming-Tsang Huang, Yeou-Lih Biofilms Anti-Bacterial Agents Antioxidants Selenium Animals Staphylococcus aureus Nanoparticles Microbial Sensitivity Tests Brachyura Hydrothermal Vents Escherichia coli Staphylococcus epidermidis Polysaccharides, Bacterial Selenium nanoparticles conjugated with exopolysaccharides from marine hydrothermal vent crab-associated actinomycete Pseudonocardia alni SCSW01 and their antioxidant, antibacterial, and antibiofilm activities. Munir Ahamed, Johnthini Dayalan, Sandhiya Dahms, Hans-Uwe Khan, Muhammad Bilal Upadhyaya, Ujala Wu, Ming-Tsang Huang, Yeou-Lih Biofilms Anti-Bacterial Agents Antioxidants Selenium Animals Staphylococcus aureus Nanoparticles Microbial Sensitivity Tests Brachyura Hydrothermal Vents Escherichia coli Staphylococcus epidermidis Polysaccharides, Bacterial Multidrug-resistant infections demand agents that combine antibacterial and antibiofilm activity. We synthesized selenium nanoparticles (SeNPs) and conjugated them with exopolysaccharides (EPS) from the shallow-water hydrothermal vent actinomycete Pseudonocardia alni SCSW01. Culture optimization increased EPS yield to 4.05 g/L. Physicochemical and microscopic analyses confirmed the formation of stable SeNPs-EPS. SeNPs exhibited a crystalline structure, whereas EPS was amorphous, and conjugation produced a negative surface potential (ζ ≈ -25 mV), indicating enhanced colloidal stability. Functionally, EPS exhibited the highest antioxidant capacity, whereas SeNPs-EPS demonstrated the most potent antibacterial activity. At 50 μg/mL concentration, SeNPs-EPS inhibited the growth of Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli; SeNPs or EPS alone were selectively active against S. epidermidis. Field emission scanning electron microscope (FESEM) images showed that SeNPs, SeNPs-EPS, and EPS caused substantial morphological damage in S. aureus. Confocal laser scanning microscopy images and antibiofilm assay results confirmed that SeNPs-EPS exhibited strong antibiofilm activity against S. aureus and E. coli. To our knowledge, this is the first report of EPS production, medium optimization, and SeNPs-EPS conjugation from Pseudonocardia alni SCSW01. Our study results indicate that SeNPs-EPS is a promising anti-biofilm coating candidate for medical devices. |
| title | Selenium nanoparticles conjugated with exopolysaccharides from marine hydrothermal vent crab-associated actinomycete Pseudonocardia alni SCSW01 and their antioxidant, antibacterial, and antibiofilm activities. |
| topic | Biofilms Anti-Bacterial Agents Antioxidants Selenium Animals Staphylococcus aureus Nanoparticles Microbial Sensitivity Tests Brachyura Hydrothermal Vents Escherichia coli Staphylococcus epidermidis Polysaccharides, Bacterial |
| url | https://pubmed.ncbi.nlm.nih.gov/41242058/ |