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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Biomaterials advances
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41242058/ |
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Table of Contents:
- Selenium nanoparticles conjugated with exopolysaccharides from marine hydrothermal vent crab-associated actinomycete Pseudonocardia alni SCSW01 and their antioxidant, antibacterial, and antibiofilm activities. Munir Ahamed, Johnthini Dayalan, Sandhiya Dahms, Hans-Uwe Khan, Muhammad Bilal Upadhyaya, Ujala Wu, Ming-Tsang Huang, Yeou-Lih Biofilms Anti-Bacterial Agents Antioxidants Selenium Animals Staphylococcus aureus Nanoparticles Microbial Sensitivity Tests Brachyura Hydrothermal Vents Escherichia coli Staphylococcus epidermidis Polysaccharides, Bacterial Multidrug-resistant infections demand agents that combine antibacterial and antibiofilm activity. We synthesized selenium nanoparticles (SeNPs) and conjugated them with exopolysaccharides (EPS) from the shallow-water hydrothermal vent actinomycete Pseudonocardia alni SCSW01. Culture optimization increased EPS yield to 4.05 g/L. Physicochemical and microscopic analyses confirmed the formation of stable SeNPs-EPS. SeNPs exhibited a crystalline structure, whereas EPS was amorphous, and conjugation produced a negative surface potential (ζ ≈ -25 mV), indicating enhanced colloidal stability. Functionally, EPS exhibited the highest antioxidant capacity, whereas SeNPs-EPS demonstrated the most potent antibacterial activity. At 50 μg/mL concentration, SeNPs-EPS inhibited the growth of Staphylococcus aureus, Staphylococcus epidermidis, and Escherichia coli; SeNPs or EPS alone were selectively active against S. epidermidis. Field emission scanning electron microscope (FESEM) images showed that SeNPs, SeNPs-EPS, and EPS caused substantial morphological damage in S. aureus. Confocal laser scanning microscopy images and antibiofilm assay results confirmed that SeNPs-EPS exhibited strong antibiofilm activity against S. aureus and E. coli. To our knowledge, this is the first report of EPS production, medium optimization, and SeNPs-EPS conjugation from Pseudonocardia alni SCSW01. Our study results indicate that SeNPs-EPS is a promising anti-biofilm coating candidate for medical devices.