Non-proteinogenic β-alanine metabolism in sp. MNB13 regulates manganese reduction in deep-sea ferromanganese nodules.
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| Format: | Artículo científico |
| Langue: | en |
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Applied and environmental microbiology
2025
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| _version_ | 1868266125103464449 |
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| author | Guo, Shuju Xu, Xiuli Shuai, Hui Song, Fuhang Zhang, Guoliang Ma, Linlin Yang, Na |
| author_facet | Guo, Shuju Xu, Xiuli Shuai, Hui Song, Fuhang Zhang, Guoliang Ma, Linlin Yang, Na Guo, Shuju Xu, Xiuli Shuai, Hui Song, Fuhang Zhang, Guoliang Ma, Linlin Yang, Na |
| collection | PubMed - marine biology |
| contents | Non-proteinogenic β-alanine metabolism in sp. MNB13 regulates manganese reduction in deep-sea ferromanganese nodules. Guo, Shuju Xu, Xiuli Shuai, Hui Song, Fuhang Zhang, Guoliang Ma, Linlin Yang, Na beta-Alanine Halomonas Manganese Oxidation-Reduction Iron Seawater Energy Metabolism Deep-sea ferromanganese nodules are valuable materials for investigating microorganism-mediated geochemical cycling of manganese. While many microbial metabolic pathways are closely associated with manganese reduction, few studies have examined the role of amino acid metabolism in the release of Mn(II) from these nodules. Here, we explored the impact of non-proteinogenic β-alanine metabolism on manganese reduction. sp. MNB13 is an indigenous bacterium isolated from a ferromanganese nodule that can reduce Mn oxides and decrease the pH of the environment during Mn(II) release. Comparative transcriptomic analysis unveiled that exposure to MnO significantly upregulated β-alanine metabolism, leading to a reduced intracellular concentration of β-alanine. Exogenous β-alanine decreased the proportion of Mn(IV) without changing the type of Mn residues by promoting MNB13 growth and reshaping the profile of secreted organic acids. β-Alanine elevated energy metabolism in MNB13 and increased catalase activity, mitigating reactive oxygen species generated during Mn(II) release and thereby enhancing bacterial growth. Among the secreted organic acids, levulinate and pantothenate showed altered abundance and were confirmed to promote Mn(II) release following β-alanine supplementation. This study suggests that environmental β-alanine modulates Mn(IV) reduction by promoting bacterial growth and organic acid secretion, offering novel insights into bacteria-mediated Mn(II) release from deep-sea ferromanganese nodules. Microorganisms are believed to play a role in the biotic processes of deep-sea ferromanganese nodule formation and biosolubilization. Although most studies have linked microbial metabolism to manganese reduction, the role of microbial amino acid metabolism in the release of Mn(Ⅱ) from ferromanganese nodules into seawater remains underexplored. β-Alanine is a naturally occurring non-proteinogenic β-amino acid widely distributed in the marine environment. However, its role in Mn(Ⅳ) reduction is unclear. This study demonstrated that Mn oxides upregulate β-alanine metabolism in sp. MNB13 and external β-alanine significantly enhances Mn(Ⅱ) release from Mn oxides. We showed that ferromanganese nodules induce β-alanine metabolism and lead to the release of pantothenate and levulinate, decreasing the culture pH, reducing Mn(Ⅳ) to Mn(Ⅱ), and resulting in Mn(Ⅱ) release. These findings provide new insights into bacterial non-proteinogenic amino acid metabolism and its role in facilitating the Mn(Ⅱ) release from ferromanganese nodules in deep-sea environments. |
| format | Artículo científico |
| id | pubmed_41251340 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Applied and environmental microbiology |
| record_format | pubmed |
| spellingShingle | Non-proteinogenic β-alanine metabolism in sp. MNB13 regulates manganese reduction in deep-sea ferromanganese nodules. Guo, Shuju Xu, Xiuli Shuai, Hui Song, Fuhang Zhang, Guoliang Ma, Linlin Yang, Na beta-Alanine Halomonas Manganese Oxidation-Reduction Iron Seawater Energy Metabolism Non-proteinogenic β-alanine metabolism in sp. MNB13 regulates manganese reduction in deep-sea ferromanganese nodules. Guo, Shuju Xu, Xiuli Shuai, Hui Song, Fuhang Zhang, Guoliang Ma, Linlin Yang, Na beta-Alanine Halomonas Manganese Oxidation-Reduction Iron Seawater Energy Metabolism Deep-sea ferromanganese nodules are valuable materials for investigating microorganism-mediated geochemical cycling of manganese. While many microbial metabolic pathways are closely associated with manganese reduction, few studies have examined the role of amino acid metabolism in the release of Mn(II) from these nodules. Here, we explored the impact of non-proteinogenic β-alanine metabolism on manganese reduction. sp. MNB13 is an indigenous bacterium isolated from a ferromanganese nodule that can reduce Mn oxides and decrease the pH of the environment during Mn(II) release. Comparative transcriptomic analysis unveiled that exposure to MnO significantly upregulated β-alanine metabolism, leading to a reduced intracellular concentration of β-alanine. Exogenous β-alanine decreased the proportion of Mn(IV) without changing the type of Mn residues by promoting MNB13 growth and reshaping the profile of secreted organic acids. β-Alanine elevated energy metabolism in MNB13 and increased catalase activity, mitigating reactive oxygen species generated during Mn(II) release and thereby enhancing bacterial growth. Among the secreted organic acids, levulinate and pantothenate showed altered abundance and were confirmed to promote Mn(II) release following β-alanine supplementation. This study suggests that environmental β-alanine modulates Mn(IV) reduction by promoting bacterial growth and organic acid secretion, offering novel insights into bacteria-mediated Mn(II) release from deep-sea ferromanganese nodules. Microorganisms are believed to play a role in the biotic processes of deep-sea ferromanganese nodule formation and biosolubilization. Although most studies have linked microbial metabolism to manganese reduction, the role of microbial amino acid metabolism in the release of Mn(Ⅱ) from ferromanganese nodules into seawater remains underexplored. β-Alanine is a naturally occurring non-proteinogenic β-amino acid widely distributed in the marine environment. However, its role in Mn(Ⅳ) reduction is unclear. This study demonstrated that Mn oxides upregulate β-alanine metabolism in sp. MNB13 and external β-alanine significantly enhances Mn(Ⅱ) release from Mn oxides. We showed that ferromanganese nodules induce β-alanine metabolism and lead to the release of pantothenate and levulinate, decreasing the culture pH, reducing Mn(Ⅳ) to Mn(Ⅱ), and resulting in Mn(Ⅱ) release. These findings provide new insights into bacterial non-proteinogenic amino acid metabolism and its role in facilitating the Mn(Ⅱ) release from ferromanganese nodules in deep-sea environments. |
| title | Non-proteinogenic β-alanine metabolism in sp. MNB13 regulates manganese reduction in deep-sea ferromanganese nodules. |
| topic | beta-Alanine Halomonas Manganese Oxidation-Reduction Iron Seawater Energy Metabolism |
| url | https://pubmed.ncbi.nlm.nih.gov/41251340/ |