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| Main Authors: | , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Comparative biochemistry and physiology. Part D, Genomics & proteomics
2026
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41265056/ |
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Table of Contents:
- The effects of interfering with gs and gls genes on intestinal genes expression and metabolic pathways in Trachinotus blochii. Chen, Changlin Wang, Lei Deng, Chaoyue Lin, Wenyan Chen, Huapeng Wang, Xinxin Yu, Haoran Sun, Junlong Luo, Jian Song, Feibiao Animals Intestines Metabolic Networks and Pathways Fish Proteins Glutamate-Cysteine Ligase RNA Interference Transcriptome Previous research has demonstrated that the addition of exogenous glutamine in feed can enhance the growth and development of Trachinotus blochii. Nonetheless, there is limited knowledge about the specific regulatory pathways and essential regulatory genes implicated in glutamine's influence on the growth and development of T. blochii. This research utilized RNA interference technology to achieve sustained suppression of the gs and gls genes in live T. blochii. After 52 days of interference, we analyzed the growth and development of T. blochii, intestinal morphology, digestive and antioxidant enzyme activity, and intestinal transcriptome. The development of T. blochii was markedly inhibited by interference, with the intestinal diameter, villus length, width, and muscle layer in the interference group being greatly reduced compared to the control group. Measurements of intestinal enzyme activity showed a significant decrease in LPS content in T. blochii following interference, and antioxidant-related enzyme activities (T-AOC and GSH) were lowered, and MDA content was significantly raised. Transcriptome analysis indicated that following the knockdown of the gs and gls genes, there was a significant enrichment of growth-related serine metabolism pathways and lipid metabolism pathways, as well as immune inflammation-related pathways, including the complement and coagulation cascade and PPAR signaling pathway. Co-expression mRNA network analysis revealed a significant enrichment of genes associated with purine de novo synthesis and the coenzyme Q oxidoreductase family. In summary, knockdown of the gs and gls genes diminishes amino acid metabolism and fat utilization in the intestines of T. blochii, thus impacting intestinal structure and hindering growth. These findings offer significant insights for the control of intestinal health and agricultural practices of T. blochii.