Astaxanthin mitigates the inflammatory toxicity of microcystin-LR on zebrafish embryos.

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Auteurs principaux: Wu, Yahui, Huang, Qing
Format: Artículo científico
Langue:en
Publié: Comparative biochemistry and physiology. Toxicology & pharmacology : CBP 2026
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author Wu, Yahui
Huang, Qing
author_facet Wu, Yahui
Huang, Qing
Wu, Yahui
Huang, Qing
collection PubMed - marine biology
contents Astaxanthin mitigates the inflammatory toxicity of microcystin-LR on zebrafish embryos. Wu, Yahui Huang, Qing Animals Microcystins Zebrafish Xanthophylls Marine Toxins Embryo, Nonmammalian Anti-Inflammatory Agents Inflammation Oxidative Stress Cyanobacteria Toxins Antioxidants Cytokines Zebrafish Proteins Astaxanthin (AST) as a natural carotenoid exhibits potent antioxidant and anti-inflammatory capacities. This work investigated AST's protective effects against microcystin-LR (MC-LR) toxicity to zebrafish embryos. When the zebrafish embryos were exposed to a sublethal, environmentally relevant concentration of MC-LR (10 μg/L, approximately half of the LC value), AST (100 μg/L) could significantly reduce MC-LR-induced mortality by 39.8% and deformity rates by 60.0%. Furthermore, AST decreased ROS and MDA levels by 11.0% and 14.5%, respectively, and enhanced the activities of superoxide dismutase (SOD, 4.4-fold), catalase (CAT, 1.2-fold), and glutathione reductase (GR, 1.6-fold). It also ameliorated MC-LR-induced inflammatory responses, as evidenced by a 49.1% reduction in neutral red staining, a 42.0% to 42.9% improvement in host resistance, and a significant down-regulation of major cytokines (IL-1β, IL-6, IL-8, TNF-α) by 0.4 to 0.6-fold. Analysis of the transcriptome revealed that AST can inhibit the C-type lectin receptor signaling pathway and others to counteract the inflammatory and oxidative stress induced by MC-LR. Our findings confirm that AST neutralizes the toxicity of MC-LR through the mechanisms of antagonizing oxidative stress, exhibiting anti-inflammatory and immunomodulatory effects, which may pave the way for AST being used in aquaculture and environmental health.
format Artículo científico
id pubmed_41270878
institution PubMed
language en
publishDate 2026
publisher Comparative biochemistry and physiology. Toxicology & pharmacology : CBP
record_format pubmed
spellingShingle Astaxanthin mitigates the inflammatory toxicity of microcystin-LR on zebrafish embryos.
Wu, Yahui
Huang, Qing
Animals
Microcystins
Zebrafish
Xanthophylls
Marine Toxins
Embryo, Nonmammalian
Anti-Inflammatory Agents
Inflammation
Oxidative Stress
Cyanobacteria Toxins
Antioxidants
Cytokines
Zebrafish Proteins
Astaxanthin mitigates the inflammatory toxicity of microcystin-LR on zebrafish embryos. Wu, Yahui Huang, Qing Animals Microcystins Zebrafish Xanthophylls Marine Toxins Embryo, Nonmammalian Anti-Inflammatory Agents Inflammation Oxidative Stress Cyanobacteria Toxins Antioxidants Cytokines Zebrafish Proteins Astaxanthin (AST) as a natural carotenoid exhibits potent antioxidant and anti-inflammatory capacities. This work investigated AST's protective effects against microcystin-LR (MC-LR) toxicity to zebrafish embryos. When the zebrafish embryos were exposed to a sublethal, environmentally relevant concentration of MC-LR (10 μg/L, approximately half of the LC value), AST (100 μg/L) could significantly reduce MC-LR-induced mortality by 39.8% and deformity rates by 60.0%. Furthermore, AST decreased ROS and MDA levels by 11.0% and 14.5%, respectively, and enhanced the activities of superoxide dismutase (SOD, 4.4-fold), catalase (CAT, 1.2-fold), and glutathione reductase (GR, 1.6-fold). It also ameliorated MC-LR-induced inflammatory responses, as evidenced by a 49.1% reduction in neutral red staining, a 42.0% to 42.9% improvement in host resistance, and a significant down-regulation of major cytokines (IL-1β, IL-6, IL-8, TNF-α) by 0.4 to 0.6-fold. Analysis of the transcriptome revealed that AST can inhibit the C-type lectin receptor signaling pathway and others to counteract the inflammatory and oxidative stress induced by MC-LR. Our findings confirm that AST neutralizes the toxicity of MC-LR through the mechanisms of antagonizing oxidative stress, exhibiting anti-inflammatory and immunomodulatory effects, which may pave the way for AST being used in aquaculture and environmental health.
title Astaxanthin mitigates the inflammatory toxicity of microcystin-LR on zebrafish embryos.
topic Animals
Microcystins
Zebrafish
Xanthophylls
Marine Toxins
Embryo, Nonmammalian
Anti-Inflammatory Agents
Inflammation
Oxidative Stress
Cyanobacteria Toxins
Antioxidants
Cytokines
Zebrafish Proteins
url https://pubmed.ncbi.nlm.nih.gov/41270878/