Cryo-EM structures of photocomplexes from the free-living aerobic anoxygenic phototrophic bacterium Erythrobacter sanguineus.

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Main Authors: Yue, Xing-Yu, Wang, Guang-Lei, Zou, Mei-Juan, Ma, Fei, Wang-Otomo, Zheng-Yu, Madigan, Michael T, Yu, Long-Jiang
Format: Artículo científico
Language:en
Published: Structure (London, England : 1993) 2026
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author Yue, Xing-Yu
Wang, Guang-Lei
Zou, Mei-Juan
Ma, Fei
Wang-Otomo, Zheng-Yu
Madigan, Michael T
Yu, Long-Jiang
author_facet Yue, Xing-Yu
Wang, Guang-Lei
Zou, Mei-Juan
Ma, Fei
Wang-Otomo, Zheng-Yu
Madigan, Michael T
Yu, Long-Jiang
Yue, Xing-Yu
Wang, Guang-Lei
Zou, Mei-Juan
Ma, Fei
Wang-Otomo, Zheng-Yu
Madigan, Michael T
Yu, Long-Jiang
collection PubMed - marine biology
contents Cryo-EM structures of photocomplexes from the free-living aerobic anoxygenic phototrophic bacterium Erythrobacter sanguineus. Yue, Xing-Yu Wang, Guang-Lei Zou, Mei-Juan Ma, Fei Wang-Otomo, Zheng-Yu Madigan, Michael T Yu, Long-Jiang Cryoelectron Microscopy Light-Harvesting Protein Complexes Bacterial Proteins Models, Molecular Carotenoids Protein Binding Sphingomonadaceae Photosynthesis Aerobic anoxygenic phototrophic bacteria (AAPB) are widely distributed in nature and they are important members of the marine phototrophic community. However, a structural and functional understanding of the AAPB photosynthetic apparatus is still lacking. Here, we present cryo-EM structures of the LH1-RC (core) and LH2 (peripheral) photocomplexes from the model aerobic phototroph Erythrobacter (Ery.) sanguineus. The LH1 αβ-heterodimers bind the carotenoids bacteriorubixanthinal and caloxanthin-pigments that are absent from anaerobic anoxygenic phototrophs-to form a closed ring structure. Ery. sanguineus LH1-RC contains a lipid-anchored polypeptide unrelated to any of the auxiliary proteins identified in the core complexes of purple bacteria so far. The Ery. sanguineus LH2 complex shows unique absorption characteristics, with its Q transition being blue-shifted to 814 nm. This work provides structural insights into the unusual photosynthetic properties of AAPB and points to new avenues to further explore their biology.
format Artículo científico
id pubmed_41274286
institution PubMed
language en
publishDate 2026
publisher Structure (London, England : 1993)
record_format pubmed
spellingShingle Cryo-EM structures of photocomplexes from the free-living aerobic anoxygenic phototrophic bacterium Erythrobacter sanguineus.
Yue, Xing-Yu
Wang, Guang-Lei
Zou, Mei-Juan
Ma, Fei
Wang-Otomo, Zheng-Yu
Madigan, Michael T
Yu, Long-Jiang
Cryoelectron Microscopy
Light-Harvesting Protein Complexes
Bacterial Proteins
Models, Molecular
Carotenoids
Protein Binding
Sphingomonadaceae
Photosynthesis
Cryo-EM structures of photocomplexes from the free-living aerobic anoxygenic phototrophic bacterium Erythrobacter sanguineus. Yue, Xing-Yu Wang, Guang-Lei Zou, Mei-Juan Ma, Fei Wang-Otomo, Zheng-Yu Madigan, Michael T Yu, Long-Jiang Cryoelectron Microscopy Light-Harvesting Protein Complexes Bacterial Proteins Models, Molecular Carotenoids Protein Binding Sphingomonadaceae Photosynthesis Aerobic anoxygenic phototrophic bacteria (AAPB) are widely distributed in nature and they are important members of the marine phototrophic community. However, a structural and functional understanding of the AAPB photosynthetic apparatus is still lacking. Here, we present cryo-EM structures of the LH1-RC (core) and LH2 (peripheral) photocomplexes from the model aerobic phototroph Erythrobacter (Ery.) sanguineus. The LH1 αβ-heterodimers bind the carotenoids bacteriorubixanthinal and caloxanthin-pigments that are absent from anaerobic anoxygenic phototrophs-to form a closed ring structure. Ery. sanguineus LH1-RC contains a lipid-anchored polypeptide unrelated to any of the auxiliary proteins identified in the core complexes of purple bacteria so far. The Ery. sanguineus LH2 complex shows unique absorption characteristics, with its Q transition being blue-shifted to 814 nm. This work provides structural insights into the unusual photosynthetic properties of AAPB and points to new avenues to further explore their biology.
title Cryo-EM structures of photocomplexes from the free-living aerobic anoxygenic phototrophic bacterium Erythrobacter sanguineus.
topic Cryoelectron Microscopy
Light-Harvesting Protein Complexes
Bacterial Proteins
Models, Molecular
Carotenoids
Protein Binding
Sphingomonadaceae
Photosynthesis
url https://pubmed.ncbi.nlm.nih.gov/41274286/