A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context.
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| Main Authors: | , , , , , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
bioRxiv : the preprint server for biology
2025
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| _version_ | 1868266122556473346 |
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| author | Bao, Yujin Marin, Zach Chen, Xiongchao Liu, Qiong Tuna, Yazgan Stoller, Sylvi Schueder, Florian Zhang, Chuyue Fung, Suet Yin Sarah Wu, Min Neugebauer, Karla M Howard, Jonathon Liu, Chi Baddeley, David Shribak, Michael Bewersdorf, Joerg |
| author_facet | Bao, Yujin Marin, Zach Chen, Xiongchao Liu, Qiong Tuna, Yazgan Stoller, Sylvi Schueder, Florian Zhang, Chuyue Fung, Suet Yin Sarah Wu, Min Neugebauer, Karla M Howard, Jonathon Liu, Chi Baddeley, David Shribak, Michael Bewersdorf, Joerg Bao, Yujin Marin, Zach Chen, Xiongchao Liu, Qiong Tuna, Yazgan Stoller, Sylvi Schueder, Florian Zhang, Chuyue Fung, Suet Yin Sarah Wu, Min Neugebauer, Karla M Howard, Jonathon Liu, Chi Baddeley, David Shribak, Michael Bewersdorf, Joerg |
| collection | PubMed - marine biology |
| contents | A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context. Bao, Yujin Marin, Zach Chen, Xiongchao Liu, Qiong Tuna, Yazgan Stoller, Sylvi Schueder, Florian Zhang, Chuyue Fung, Suet Yin Sarah Wu, Min Neugebauer, Karla M Howard, Jonathon Liu, Chi Baddeley, David Shribak, Michael Bewersdorf, Joerg Fluorescence microscopy has been widely used to reveal the spatial distribution of specifically labeled molecules, but it is blind to cellular context. Quantitative phase contrast microscopy (QPC) provides such complementary information. Here we have developed a platform that combines the QPC technique of correlative orientation-independent differential interference contrast (OI-DIC) microscopy with single-molecule super-resolution fluorescence microscopy. We demonstrate a detection sensitivity of 0.05 nm optical path difference, sufficient to detect single microtubules, and show its capability of 3D super-resolution fluorescence imaging in the cellular context. Additionally, we report deep-learning enabled digital staining, identifying nuclei, mitochondria and lipid droplets from OI-DIC data and demonstrate the potential of this approach for long-term live-cell imaging of organelles of interest without the need for fluorescence. OI-DIC can be easily integrated into most fluorescence microscopes and is readily adoptable by microscopy labs. |
| format | Artículo científico |
| id | pubmed_41278783 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | bioRxiv : the preprint server for biology |
| record_format | pubmed |
| spellingShingle | A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context. Bao, Yujin Marin, Zach Chen, Xiongchao Liu, Qiong Tuna, Yazgan Stoller, Sylvi Schueder, Florian Zhang, Chuyue Fung, Suet Yin Sarah Wu, Min Neugebauer, Karla M Howard, Jonathon Liu, Chi Baddeley, David Shribak, Michael Bewersdorf, Joerg A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context. Bao, Yujin Marin, Zach Chen, Xiongchao Liu, Qiong Tuna, Yazgan Stoller, Sylvi Schueder, Florian Zhang, Chuyue Fung, Suet Yin Sarah Wu, Min Neugebauer, Karla M Howard, Jonathon Liu, Chi Baddeley, David Shribak, Michael Bewersdorf, Joerg Fluorescence microscopy has been widely used to reveal the spatial distribution of specifically labeled molecules, but it is blind to cellular context. Quantitative phase contrast microscopy (QPC) provides such complementary information. Here we have developed a platform that combines the QPC technique of correlative orientation-independent differential interference contrast (OI-DIC) microscopy with single-molecule super-resolution fluorescence microscopy. We demonstrate a detection sensitivity of 0.05 nm optical path difference, sufficient to detect single microtubules, and show its capability of 3D super-resolution fluorescence imaging in the cellular context. Additionally, we report deep-learning enabled digital staining, identifying nuclei, mitochondria and lipid droplets from OI-DIC data and demonstrate the potential of this approach for long-term live-cell imaging of organelles of interest without the need for fluorescence. OI-DIC can be easily integrated into most fluorescence microscopes and is readily adoptable by microscopy labs. |
| title | A correlative quantitative phase contrast and fluorescence super-resolution microscope for imaging molecules in their cellular context. |
| url | https://pubmed.ncbi.nlm.nih.gov/41278783/ |