Saved in:
| Main Authors: | , , , , , , , , , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
bioRxiv : the preprint server for biology
2025
|
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41279112/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Table of Contents:
- A chromosome-level assembly and functional genomic resources for the model annelid . Davies, Billie E Gonzalez, Paul Sur, Abhinav Wei, Jingcheng Frankish, Tom Montagne, Jimena Carrillo-Baltodano, Allan M Guynes, Kero Liang, Yan Donnellan, Rory D Travis Moreland, R Singh, Sumeeta Zhang, Suiyuan Wolfsberg, Tyra G Meyer, Néva P Seaver, Elaine C Baxevanis, Andreas D Martín-Durán, José M The polychaete is a commonly used annelid for studies in evolutionary developmental biology, comparative genomics, conservation, and ecotoxicology. Over a decade ago, it was the first polychaete to have its genome sequenced and assembled, contributing to pioneering studies that transformed our understanding of animal genomes and their evolution. However, this early resource is now outdated compared to current genome sequencing standards, limiting the use of modern functional genomic tools that could further our understanding of numerous biological processes. We combine long-read and short-read sequencing with Hi-C chromatin conformation capture data to assemble the chromosome-level nuclear and mitochondrial genomes of the laboratory strain of . This reference assembly more accurately reflects the expected genome size for this polychaete (~243.6 Mb) and contains a highly complete, evolutionarily conserved gene repertoire. Notably, the nuclear and mitochondrial genomes are heavily rearranged, indicating a decoupling between gene family repertoire and chromosomal evolution. The analyses of multi-omic datasets available for , including developmental time courses of bulk and single-cell RNA-seq, ATAC-seq, and EM-seq, using the new reference assembly, resulted in a significant quality improvement, allowing us to identify new cell-type-specific gene markers and gain additional insights of biological relevance. Finally, we generated a publicly available genome browser that ensures all these resources are easily findable, accessible, interoperable, and reusable. Our study provides state-of-the-art genomic resources for the polychaete model , addressing a pressing community need that will open new research opportunities in animal and genome evolution.