Saved in:
| Main Authors: | , , , , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
bioRxiv : the preprint server for biology
2025
|
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41279224/ |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1868266122555424770 |
|---|---|
| author | Rubino, Frederick A Coffing, Gabrielle C Gibbons, Connor J Small, Scott T Desvignes, Thomas Pessutti, Jeffery Petersen, Ann M Arkhipkin, Alexander Shcherbich, Zhanna Postlethwait, John H Kern, Andrew D Montague, Tessa G |
| author_facet | Rubino, Frederick A Coffing, Gabrielle C Gibbons, Connor J Small, Scott T Desvignes, Thomas Pessutti, Jeffery Petersen, Ann M Arkhipkin, Alexander Shcherbich, Zhanna Postlethwait, John H Kern, Andrew D Montague, Tessa G Rubino, Frederick A Coffing, Gabrielle C Gibbons, Connor J Small, Scott T Desvignes, Thomas Pessutti, Jeffery Petersen, Ann M Arkhipkin, Alexander Shcherbich, Zhanna Postlethwait, John H Kern, Andrew D Montague, Tessa G |
| collection | PubMed - marine biology |
| contents | A non-invasive method to genotype cephalopod sex by quantitative PCR. Rubino, Frederick A Coffing, Gabrielle C Gibbons, Connor J Small, Scott T Desvignes, Thomas Pessutti, Jeffery Petersen, Ann M Arkhipkin, Alexander Shcherbich, Zhanna Postlethwait, John H Kern, Andrew D Montague, Tessa G Coleoid cephalopods (cuttlefish, octopus, and squid) are emerging model organisms in neuroscience, development, and evolutionary biology, and are of major economic importance in global fisheries. However, they are notoriously difficult and expensive to culture. The ability to determine sex early in development would enable more efficient and sustainable population management in both lab and wild settings. Here, we present a non-invasive method to genotype the sex of dwarf cuttlefish () as young as three hours post-hatching using a skin swab and quantitative PCR assay, which detects a two-fold dosage difference between ZZ and Z0 sex chromosomes of males and females, respectively. Furthermore, we designed and validated primers for four additional cephalopod research species with assembled genomes (, ), and for a wild-caught species of economic value () for which we generated low-coverage whole genome sequencing data. This sex-genotyping method enables accurate sex determination from hatchlings to adults across cephalopods, independent of genome quality or availability. |
| format | Artículo científico |
| id | pubmed_41279224 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | bioRxiv : the preprint server for biology |
| record_format | pubmed |
| spellingShingle | A non-invasive method to genotype cephalopod sex by quantitative PCR. Rubino, Frederick A Coffing, Gabrielle C Gibbons, Connor J Small, Scott T Desvignes, Thomas Pessutti, Jeffery Petersen, Ann M Arkhipkin, Alexander Shcherbich, Zhanna Postlethwait, John H Kern, Andrew D Montague, Tessa G A non-invasive method to genotype cephalopod sex by quantitative PCR. Rubino, Frederick A Coffing, Gabrielle C Gibbons, Connor J Small, Scott T Desvignes, Thomas Pessutti, Jeffery Petersen, Ann M Arkhipkin, Alexander Shcherbich, Zhanna Postlethwait, John H Kern, Andrew D Montague, Tessa G Coleoid cephalopods (cuttlefish, octopus, and squid) are emerging model organisms in neuroscience, development, and evolutionary biology, and are of major economic importance in global fisheries. However, they are notoriously difficult and expensive to culture. The ability to determine sex early in development would enable more efficient and sustainable population management in both lab and wild settings. Here, we present a non-invasive method to genotype the sex of dwarf cuttlefish () as young as three hours post-hatching using a skin swab and quantitative PCR assay, which detects a two-fold dosage difference between ZZ and Z0 sex chromosomes of males and females, respectively. Furthermore, we designed and validated primers for four additional cephalopod research species with assembled genomes (, ), and for a wild-caught species of economic value () for which we generated low-coverage whole genome sequencing data. This sex-genotyping method enables accurate sex determination from hatchlings to adults across cephalopods, independent of genome quality or availability. |
| title | A non-invasive method to genotype cephalopod sex by quantitative PCR. |
| url | https://pubmed.ncbi.nlm.nih.gov/41279224/ |