Creating an Improved Diatoxanthin Production Line by Knocking Out in the Background in the Marine Diatom .
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| Format: | Artículo científico |
| Sprache: | en |
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Marine drugs
2025
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| author | Volpe, Charlotte Bartosova, Zdenka Kissen, Ralph Winge, Per Nymark, Marianne |
| author_facet | Volpe, Charlotte Bartosova, Zdenka Kissen, Ralph Winge, Per Nymark, Marianne Volpe, Charlotte Bartosova, Zdenka Kissen, Ralph Winge, Per Nymark, Marianne |
| collection | PubMed - marine biology |
| contents | Creating an Improved Diatoxanthin Production Line by Knocking Out in the Background in the Marine Diatom . Volpe, Charlotte Bartosova, Zdenka Kissen, Ralph Winge, Per Nymark, Marianne Diatoms Xanthophylls Microalgae Oxidoreductases Light Mutation Diatoxanthin is a photoprotective carotenoid found in a few groups of microalgae displaying in vitro anti-inflammatory and anti-cancer properties, making it a promising candidate for nutraceutical, pharmaceutical, and cosmetic applications. However, large-scale production is currently nonexistent because of two major challenges: Instability during microalgae harvesting, where diatoxanthin is rapidly converted back to its inactive precursor diadinoxanthin under non-stressful light conditions, and dependence on prolonged exposure to high-intensity light, which is costly and technically challenging during indoor high-cell-density cultivation. The first limitation was previously addressed by knocking out zeaxanthin epoxidase 3 (ZEP3) in the marine diatom , resulting in a mutant that stabilized diatoxanthin under non-stressful light conditions. Here, we report an improved diatoxanthin production line where both of the described challenges have been overcome. This was achieved by creating mutants where the phenotype of the mutant was combined with the light-sensitive phenotype of the () mutant. Growth rates were maintained at wild-type levels at light intensities ≤ 150 µmol photons m s in the mutants, but prolonged medium light exposure resulted in a 1.5- and 7-fold increase in diatoxanthin concentration compared with and wild-type, respectively. When returned to low light, the cultures retained ~80% of their accumulated diatoxanthin. The improved production lines allow for diatoxanthin accumulation without the use of high-intensity light and with limited loss of diatoxanthin when returned to non-stressful light conditions. |
| format | Artículo científico |
| id | pubmed_41295387 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Marine drugs |
| record_format | pubmed |
| spellingShingle | Creating an Improved Diatoxanthin Production Line by Knocking Out in the Background in the Marine Diatom . Volpe, Charlotte Bartosova, Zdenka Kissen, Ralph Winge, Per Nymark, Marianne Diatoms Xanthophylls Microalgae Oxidoreductases Light Mutation Creating an Improved Diatoxanthin Production Line by Knocking Out in the Background in the Marine Diatom . Volpe, Charlotte Bartosova, Zdenka Kissen, Ralph Winge, Per Nymark, Marianne Diatoms Xanthophylls Microalgae Oxidoreductases Light Mutation Diatoxanthin is a photoprotective carotenoid found in a few groups of microalgae displaying in vitro anti-inflammatory and anti-cancer properties, making it a promising candidate for nutraceutical, pharmaceutical, and cosmetic applications. However, large-scale production is currently nonexistent because of two major challenges: Instability during microalgae harvesting, where diatoxanthin is rapidly converted back to its inactive precursor diadinoxanthin under non-stressful light conditions, and dependence on prolonged exposure to high-intensity light, which is costly and technically challenging during indoor high-cell-density cultivation. The first limitation was previously addressed by knocking out zeaxanthin epoxidase 3 (ZEP3) in the marine diatom , resulting in a mutant that stabilized diatoxanthin under non-stressful light conditions. Here, we report an improved diatoxanthin production line where both of the described challenges have been overcome. This was achieved by creating mutants where the phenotype of the mutant was combined with the light-sensitive phenotype of the () mutant. Growth rates were maintained at wild-type levels at light intensities ≤ 150 µmol photons m s in the mutants, but prolonged medium light exposure resulted in a 1.5- and 7-fold increase in diatoxanthin concentration compared with and wild-type, respectively. When returned to low light, the cultures retained ~80% of their accumulated diatoxanthin. The improved production lines allow for diatoxanthin accumulation without the use of high-intensity light and with limited loss of diatoxanthin when returned to non-stressful light conditions. |
| title | Creating an Improved Diatoxanthin Production Line by Knocking Out in the Background in the Marine Diatom . |
| topic | Diatoms Xanthophylls Microalgae Oxidoreductases Light Mutation |
| url | https://pubmed.ncbi.nlm.nih.gov/41295387/ |