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| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Marine drugs
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41295410/ |
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Table of Contents:
- as a Platform to Assess the Cytotoxicity of Marine Algal Extracts: The Case of . Figueroa-Valencia, Sheyla J Hernández, Marcos Castañeta, Grover Pérez, Ian Ardiles, Alejandro Figueroa-Valencia, Elizabeth Terrones, Teresa Cano de Chávez, Francisco P Areche, Carlos Seaweed Dictyostelium Biological Products Tandem Mass Spectrometry High-Throughput Screening Assays Diterpenes Chromatography, High Pressure Liquid Plant Extracts Drug Discovery The social amoeba is a versatile biological model widely used in drug discovery and studying cellular stress responses. However, its application for cytotoxicity evaluation of natural products, particularly algal-derived compounds, remains underutilized. In this study, we developed a high-throughput developmental assay in to analyze the cytotoxicity of acetone and methanol extracts from the Peruvian seaweed . Our results showed that the acetone extract caused a transient delay in the social development of the amoeba. In contrast, the methanol extract exhibited no significant effects, even at high extract concentrations. UHPLC/Orbitrap/ESI/MS/MS analysis tentatively identified ten major compounds, including pachydictyol A and dictyotriol A diacetate. The presence of diterpenes, such as dictyotadiol and pachydictyol A, previously reported to exhibit moderate cytotoxic activity, likely explains the developmental delay observed with the acetone extract. This study highlights the utility of as a scalable cytotoxicity screening platform within algal pharmacognosy, facilitating the early identification of non-toxic marine natural products suitable for further biomedical and biotechnological development.