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| Format: | Artículo científico |
| Sprache: | en |
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Biomedical engineering online
2025
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| Online-Zugang: | https://pubmed.ncbi.nlm.nih.gov/41299692/ |
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| author | Ahrari, Ameneh Khodabandeh, Saber Akhavan Niaki, Haleh Zare-Zardini, Hadi |
| author_facet | Ahrari, Ameneh Khodabandeh, Saber Akhavan Niaki, Haleh Zare-Zardini, Hadi Ahrari, Ameneh Khodabandeh, Saber Akhavan Niaki, Haleh Zare-Zardini, Hadi |
| collection | PubMed - marine biology |
| contents | Inhibition of Na,K -ATPase enzyme by tetrodotoxin extract in the breast tumor xenograft Balb/c nu mouse model. Ahrari, Ameneh Khodabandeh, Saber Akhavan Niaki, Haleh Zare-Zardini, Hadi Animals Mice Breast Neoplasms Female Tetrodotoxin Sodium-Potassium-Exchanging ATPase Cell Line, Tumor Mice, Inbred BALB C Enzyme Inhibitors Xenograft Model Antitumor Assays The Na, K-ATPase (NKA) is involved in many cellular functions, and changes in its activity and expression have been linked to various diseases, including cancer. This study aims to investigate the potential anti-tumor effect of tetrodotoxin (TTX), a potent sodium channel blocker extracted from the pufferfish Chelonodon patoca, on nude mice (Balb/c nu) induced with breast cancer (4T1) to determine whether it can affect the activity of the NKA. TTX was extracted using 2% acetic acid-methanol (v/v). To determine the lethal concentration of TTX, three concentrations of the TTX extract (1.5, 2.0, and 3.0 mg/kg) were injected into transgenic mice that had already been induced by the 4T1 cell line and bearing tumors. After 7-8 weeks of monitoring tumor volume, the mice were killed, and a portion of the tumors was fixed in Bouin's fixative for immunohistological analysis, while the rest was frozen for biochemical analysis. Biometric analysis of Balb/c nu mice indicated a notable reduction in tumor volume progression in the group subjected to TTX extract compared to the control group. Furthermore, immunohistochemical and biochemical analysis demonstrated a decrease in the intensity and activity of NKA in the TTX extract group compared to the control group. Our results indicate that TTX extract may have anti-tumor effects by inhibiting NKA activity. Therefore, considering the presence of TTX in the pufferfish extract, TTX could be a promising therapeutic candidate for controlling metastatic breast tumor growth and metastasis through NKA inhibition. |
| format | Artículo científico |
| id | pubmed_41299692 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Biomedical engineering online |
| record_format | pubmed |
| spellingShingle | Inhibition of Na,K -ATPase enzyme by tetrodotoxin extract in the breast tumor xenograft Balb/c nu mouse model. Ahrari, Ameneh Khodabandeh, Saber Akhavan Niaki, Haleh Zare-Zardini, Hadi Animals Mice Breast Neoplasms Female Tetrodotoxin Sodium-Potassium-Exchanging ATPase Cell Line, Tumor Mice, Inbred BALB C Enzyme Inhibitors Xenograft Model Antitumor Assays Inhibition of Na,K -ATPase enzyme by tetrodotoxin extract in the breast tumor xenograft Balb/c nu mouse model. Ahrari, Ameneh Khodabandeh, Saber Akhavan Niaki, Haleh Zare-Zardini, Hadi Animals Mice Breast Neoplasms Female Tetrodotoxin Sodium-Potassium-Exchanging ATPase Cell Line, Tumor Mice, Inbred BALB C Enzyme Inhibitors Xenograft Model Antitumor Assays The Na, K-ATPase (NKA) is involved in many cellular functions, and changes in its activity and expression have been linked to various diseases, including cancer. This study aims to investigate the potential anti-tumor effect of tetrodotoxin (TTX), a potent sodium channel blocker extracted from the pufferfish Chelonodon patoca, on nude mice (Balb/c nu) induced with breast cancer (4T1) to determine whether it can affect the activity of the NKA. TTX was extracted using 2% acetic acid-methanol (v/v). To determine the lethal concentration of TTX, three concentrations of the TTX extract (1.5, 2.0, and 3.0 mg/kg) were injected into transgenic mice that had already been induced by the 4T1 cell line and bearing tumors. After 7-8 weeks of monitoring tumor volume, the mice were killed, and a portion of the tumors was fixed in Bouin's fixative for immunohistological analysis, while the rest was frozen for biochemical analysis. Biometric analysis of Balb/c nu mice indicated a notable reduction in tumor volume progression in the group subjected to TTX extract compared to the control group. Furthermore, immunohistochemical and biochemical analysis demonstrated a decrease in the intensity and activity of NKA in the TTX extract group compared to the control group. Our results indicate that TTX extract may have anti-tumor effects by inhibiting NKA activity. Therefore, considering the presence of TTX in the pufferfish extract, TTX could be a promising therapeutic candidate for controlling metastatic breast tumor growth and metastasis through NKA inhibition. |
| title | Inhibition of Na,K -ATPase enzyme by tetrodotoxin extract in the breast tumor xenograft Balb/c nu mouse model. |
| topic | Animals Mice Breast Neoplasms Female Tetrodotoxin Sodium-Potassium-Exchanging ATPase Cell Line, Tumor Mice, Inbred BALB C Enzyme Inhibitors Xenograft Model Antitumor Assays |
| url | https://pubmed.ncbi.nlm.nih.gov/41299692/ |