Cultivation of Bdelloid Rotifer with Synthetic Medium and Characterization of Associated Bacteria.
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| Autori principali: | , , , |
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| Natura: | Artículo científico |
| Lingua: | en |
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Biology
2025
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| _version_ | 1868266119913013249 |
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| author | Wang, Wenbo He, Zhili Wang, Qing Yang, Yufeng |
| author_facet | Wang, Wenbo He, Zhili Wang, Qing Yang, Yufeng Wang, Wenbo He, Zhili Wang, Qing Yang, Yufeng |
| collection | PubMed - marine biology |
| contents | Cultivation of Bdelloid Rotifer with Synthetic Medium and Characterization of Associated Bacteria. Wang, Wenbo He, Zhili Wang, Qing Yang, Yufeng Bdelloid rotifers are model organisms for evolutionary genetics; however, their laboratory cultivation has been limited to traditional systems that require natural food sources (e.g., lettuce juice, bacteria, or yeast) of undefined composition. This constraint impedes mechanistic studies of rotifer-microbe interactions and genetic evolution. We developed a synthetic rotifer medium (SRM) that enables axenic cultivation of , the most commonly used model species of bdelloid rotifers in the laboratory, as a chemically controlled alternative. reached a population density of 357 ± 19.95 ind./mL with a specific growth rate of 0.2131 ± 0.003 over 20 days in SRM, achieving parity with traditional food-supplemented systems while eliminating compositional variability. We further isolated 20 bacterial strains associated with SRM-cultured , which were affiliated with two genera ( and ) on the body surface, as well as four genera (, , and ) and one family (Burkholderiaceae) inside . Additionally, the addition of low-concentration antibiotics over 20 days reduced the population size or specific growth rate of , and cannot fully eliminate the associated bacteria. This study established the first nutritionally autonomous, compositionally stable culture system for bdelloids, enabling precise investigation of rotifer-microbe coevolution and functional genetics. |
| format | Artículo científico |
| id | pubmed_41300299 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Biology |
| record_format | pubmed |
| spellingShingle | Cultivation of Bdelloid Rotifer with Synthetic Medium and Characterization of Associated Bacteria. Wang, Wenbo He, Zhili Wang, Qing Yang, Yufeng Cultivation of Bdelloid Rotifer with Synthetic Medium and Characterization of Associated Bacteria. Wang, Wenbo He, Zhili Wang, Qing Yang, Yufeng Bdelloid rotifers are model organisms for evolutionary genetics; however, their laboratory cultivation has been limited to traditional systems that require natural food sources (e.g., lettuce juice, bacteria, or yeast) of undefined composition. This constraint impedes mechanistic studies of rotifer-microbe interactions and genetic evolution. We developed a synthetic rotifer medium (SRM) that enables axenic cultivation of , the most commonly used model species of bdelloid rotifers in the laboratory, as a chemically controlled alternative. reached a population density of 357 ± 19.95 ind./mL with a specific growth rate of 0.2131 ± 0.003 over 20 days in SRM, achieving parity with traditional food-supplemented systems while eliminating compositional variability. We further isolated 20 bacterial strains associated with SRM-cultured , which were affiliated with two genera ( and ) on the body surface, as well as four genera (, , and ) and one family (Burkholderiaceae) inside . Additionally, the addition of low-concentration antibiotics over 20 days reduced the population size or specific growth rate of , and cannot fully eliminate the associated bacteria. This study established the first nutritionally autonomous, compositionally stable culture system for bdelloids, enabling precise investigation of rotifer-microbe coevolution and functional genetics. |
| title | Cultivation of Bdelloid Rotifer with Synthetic Medium and Characterization of Associated Bacteria. |
| url | https://pubmed.ncbi.nlm.nih.gov/41300299/ |