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| Main Authors: | , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Biology
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41300299/ |
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Table of Contents:
- Cultivation of Bdelloid Rotifer with Synthetic Medium and Characterization of Associated Bacteria. Wang, Wenbo He, Zhili Wang, Qing Yang, Yufeng Bdelloid rotifers are model organisms for evolutionary genetics; however, their laboratory cultivation has been limited to traditional systems that require natural food sources (e.g., lettuce juice, bacteria, or yeast) of undefined composition. This constraint impedes mechanistic studies of rotifer-microbe interactions and genetic evolution. We developed a synthetic rotifer medium (SRM) that enables axenic cultivation of , the most commonly used model species of bdelloid rotifers in the laboratory, as a chemically controlled alternative. reached a population density of 357 ± 19.95 ind./mL with a specific growth rate of 0.2131 ± 0.003 over 20 days in SRM, achieving parity with traditional food-supplemented systems while eliminating compositional variability. We further isolated 20 bacterial strains associated with SRM-cultured , which were affiliated with two genera ( and ) on the body surface, as well as four genera (, , and ) and one family (Burkholderiaceae) inside . Additionally, the addition of low-concentration antibiotics over 20 days reduced the population size or specific growth rate of , and cannot fully eliminate the associated bacteria. This study established the first nutritionally autonomous, compositionally stable culture system for bdelloids, enabling precise investigation of rotifer-microbe coevolution and functional genetics.