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| Main Authors: | , , , , , , , |
|---|---|
| Format: | Artículo científico |
| Language: | en |
| Published: |
Biology
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41300336/ |
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| _version_ | 1868266119910916096 |
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| author | Liu, Yi Xie, Jiang He, Yang Shi, Qingchao Gong, Quan Zhao, Weihong Qin, Chuanjie Zhou, Chuang |
| author_facet | Liu, Yi Xie, Jiang He, Yang Shi, Qingchao Gong, Quan Zhao, Weihong Qin, Chuanjie Zhou, Chuang Liu, Yi Xie, Jiang He, Yang Shi, Qingchao Gong, Quan Zhao, Weihong Qin, Chuanjie Zhou, Chuang |
| collection | PubMed - marine biology |
| contents | Metabolome and Metagenome Signatures Underlying the Differential Resistance of , Crucian Carp, and Yellow Catfish to Infection. Liu, Yi Xie, Jiang He, Yang Shi, Qingchao Gong, Quan Zhao, Weihong Qin, Chuanjie Zhou, Chuang poses a significant threat to global aquaculture, yet some fish species exhibit remarkable resistance. This study employed a combined LC-MS-based metabolomics and 16S rRNA gene sequencing approach to investigate the intrinsic mechanisms underlying the differential susceptibility of , crucian carp, and yellow catfish. Our results revealed distinct skin molecular and microbial profiles in associated with its enhanced resilience. Metabolomic analysis identified a significant upregulation of key antioxidants (L-Glutathione reduced, L-Glutathione oxidized, L-Cysteine-glutathione gisulfide, Uric acid, Histamine, N-Acetylhistamine, and scorbic acid) in , most notably L-Glutathione reduced, which was 31- and 59-fold higher than in yellow catfish and crucian carp, respectively. Functional enrichment further highlighted the critical role of enhanced antioxidant capacity (centered on glutathione metabolism) and immune/inflammatory responses in the resistance to of . Concurrently, skin microbiome analysis showed that hosted a microbial community distinct from the other two species, with significantly higher α-diversity. Notably, skin was significantly depleted of the parasitic bacteria and , which were highly abundant in the susceptible species. Furthermore, predicted metagenomic functions indicated that 's microbiota was enriched in fundamental metabolic pathways, whereas the microbiota of crucian carp and yellow catfish was skewed towards disease- and immune-related pathways. In conclusion, our findings demonstrate that the superior resistance of to is likely conferred by a synergistic effect of a robust skin antioxidant capacity (primarily driven by glutathione) and a protective skin microbiome that excludes specific parasites. This study provides novel insights into the multi-faceted mechanisms of disease resistance in fish. |
| format | Artículo científico |
| id | pubmed_41300336 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Biology |
| record_format | pubmed |
| spellingShingle | Metabolome and Metagenome Signatures Underlying the Differential Resistance of , Crucian Carp, and Yellow Catfish to Infection. Liu, Yi Xie, Jiang He, Yang Shi, Qingchao Gong, Quan Zhao, Weihong Qin, Chuanjie Zhou, Chuang Metabolome and Metagenome Signatures Underlying the Differential Resistance of , Crucian Carp, and Yellow Catfish to Infection. Liu, Yi Xie, Jiang He, Yang Shi, Qingchao Gong, Quan Zhao, Weihong Qin, Chuanjie Zhou, Chuang poses a significant threat to global aquaculture, yet some fish species exhibit remarkable resistance. This study employed a combined LC-MS-based metabolomics and 16S rRNA gene sequencing approach to investigate the intrinsic mechanisms underlying the differential susceptibility of , crucian carp, and yellow catfish. Our results revealed distinct skin molecular and microbial profiles in associated with its enhanced resilience. Metabolomic analysis identified a significant upregulation of key antioxidants (L-Glutathione reduced, L-Glutathione oxidized, L-Cysteine-glutathione gisulfide, Uric acid, Histamine, N-Acetylhistamine, and scorbic acid) in , most notably L-Glutathione reduced, which was 31- and 59-fold higher than in yellow catfish and crucian carp, respectively. Functional enrichment further highlighted the critical role of enhanced antioxidant capacity (centered on glutathione metabolism) and immune/inflammatory responses in the resistance to of . Concurrently, skin microbiome analysis showed that hosted a microbial community distinct from the other two species, with significantly higher α-diversity. Notably, skin was significantly depleted of the parasitic bacteria and , which were highly abundant in the susceptible species. Furthermore, predicted metagenomic functions indicated that 's microbiota was enriched in fundamental metabolic pathways, whereas the microbiota of crucian carp and yellow catfish was skewed towards disease- and immune-related pathways. In conclusion, our findings demonstrate that the superior resistance of to is likely conferred by a synergistic effect of a robust skin antioxidant capacity (primarily driven by glutathione) and a protective skin microbiome that excludes specific parasites. This study provides novel insights into the multi-faceted mechanisms of disease resistance in fish. |
| title | Metabolome and Metagenome Signatures Underlying the Differential Resistance of , Crucian Carp, and Yellow Catfish to Infection. |
| url | https://pubmed.ncbi.nlm.nih.gov/41300336/ |