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| Main Authors: | , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Genes
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41300745/ |
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Table of Contents:
- Gonadal Transcriptome Analysis Reveals the lncRNA-mRNA Pair in Sea Cucumber . Zhang, Jing Yu, Jingwei Zhang, Yang Su, Meiyao Animals RNA, Long Noncoding RNA, Messenger Male Female Transcriptome Holothuria Ovary Gene Expression Profiling Testis Gonads Long non-coding RNA (lncRNA) was structurally similar to mRNAs, yet they could not be translated into proteins. While an increasing number of reports have systematically identified and described lncRNA in model species, information about non-model species remains scarce. Sea cucumber could be used for both medicinal and food purposes, which have high economic value, gradually attracting the attention of researchers. In this research, we constructed lncRNA library and compared the difference in lncRNA expression profiles between testis and ovary of sea cucumber . To elucidate the molecular interactions between lncRNA and mRNA, we computationally predicted potential complementary binding sites through analysis of both cis- and trans-acting antisense mechanisms. Subsequent Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses demonstrated that the identified target genes are potentially involved in the regulatory pathways governing gonad development. Quantitative reverse transcription PCR analysis showed that MSTRG.32831.1- and MSTRG.57315.1- exhibited a high expression pattern in testis; while MSTRG.11041.1- and MSTRG.11074.1- showed a high expression pattern in the ovary. Deciphering lncRNA-mRNA expression patterns may uncover fundamental principles governing reproductive regulation in marine invertebrates. This discovery not only deepens understanding in this field but also provides valuable comparative insights for developmental biology.