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| Main Authors: | , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
International journal of molecular sciences
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41303395/ |
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| _version_ | 1868266119906721792 |
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| author | Šamanić, Ivica Dželalija, Mia Bellulovich, Ema Kalinić, Hrvoje Jozić, Slaven Ordulj, Marin Udiković-Kolić, Nikolina Maravić, Ana |
| author_facet | Šamanić, Ivica Dželalija, Mia Bellulovich, Ema Kalinić, Hrvoje Jozić, Slaven Ordulj, Marin Udiković-Kolić, Nikolina Maravić, Ana Šamanić, Ivica Dželalija, Mia Bellulovich, Ema Kalinić, Hrvoje Jozić, Slaven Ordulj, Marin Udiković-Kolić, Nikolina Maravić, Ana |
| collection | PubMed - marine biology |
| contents | Characterisation of Plasmid-Associated Antimicrobial Resistance Genes in Coastal Marine from the Central Adriatic Sea: De Novo Assembly and Bioinformatic Profiling. Šamanić, Ivica Dželalija, Mia Bellulovich, Ema Kalinić, Hrvoje Jozić, Slaven Ordulj, Marin Udiković-Kolić, Nikolina Maravić, Ana Plasmids Computational Biology Enterobacteriaceae Drug Resistance, Bacterial Anti-Bacterial Agents Genes, Bacterial Seawater Whole Genome Sequencing Genome, Bacterial This study examines the genomic composition and resistance potential of eight putative plasmid-derived contig assemblies reconstructed from marine isolated in the central Adriatic Sea. Using a combination of Illumina-based whole genome sequencing, de novo assembly, and a multi-tool bioinformatics pipeline, we annotated antimicrobial resistance genes (ARGs), insertion sequences (ISs), and plasmid replicon types. Clinically significant resistance markers such as , , , , and were identified, frequently co-localised with mobile genetic elements including IS110, IS4, and IS1182. The plasmid-associated contigs were assigned to MOBP and MOBQ types and contained replicon markers (IncP6, IncA/C2) characteristic of broad-host-range plasmids. Our findings provide valuable insight into the plasmidome of environmental , emphasising the role of coastal pollution in shaping the distribution and potential mobility of antimicrobial resistance genes. This supports the One Health framework by linking environmental reservoirs to clinically relevant resistance mechanisms. |
| format | Artículo científico |
| id | pubmed_41303395 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of molecular sciences |
| record_format | pubmed |
| spellingShingle | Characterisation of Plasmid-Associated Antimicrobial Resistance Genes in Coastal Marine from the Central Adriatic Sea: De Novo Assembly and Bioinformatic Profiling. Šamanić, Ivica Dželalija, Mia Bellulovich, Ema Kalinić, Hrvoje Jozić, Slaven Ordulj, Marin Udiković-Kolić, Nikolina Maravić, Ana Plasmids Computational Biology Enterobacteriaceae Drug Resistance, Bacterial Anti-Bacterial Agents Genes, Bacterial Seawater Whole Genome Sequencing Genome, Bacterial Characterisation of Plasmid-Associated Antimicrobial Resistance Genes in Coastal Marine from the Central Adriatic Sea: De Novo Assembly and Bioinformatic Profiling. Šamanić, Ivica Dželalija, Mia Bellulovich, Ema Kalinić, Hrvoje Jozić, Slaven Ordulj, Marin Udiković-Kolić, Nikolina Maravić, Ana Plasmids Computational Biology Enterobacteriaceae Drug Resistance, Bacterial Anti-Bacterial Agents Genes, Bacterial Seawater Whole Genome Sequencing Genome, Bacterial This study examines the genomic composition and resistance potential of eight putative plasmid-derived contig assemblies reconstructed from marine isolated in the central Adriatic Sea. Using a combination of Illumina-based whole genome sequencing, de novo assembly, and a multi-tool bioinformatics pipeline, we annotated antimicrobial resistance genes (ARGs), insertion sequences (ISs), and plasmid replicon types. Clinically significant resistance markers such as , , , , and were identified, frequently co-localised with mobile genetic elements including IS110, IS4, and IS1182. The plasmid-associated contigs were assigned to MOBP and MOBQ types and contained replicon markers (IncP6, IncA/C2) characteristic of broad-host-range plasmids. Our findings provide valuable insight into the plasmidome of environmental , emphasising the role of coastal pollution in shaping the distribution and potential mobility of antimicrobial resistance genes. This supports the One Health framework by linking environmental reservoirs to clinically relevant resistance mechanisms. |
| title | Characterisation of Plasmid-Associated Antimicrobial Resistance Genes in Coastal Marine from the Central Adriatic Sea: De Novo Assembly and Bioinformatic Profiling. |
| topic | Plasmids Computational Biology Enterobacteriaceae Drug Resistance, Bacterial Anti-Bacterial Agents Genes, Bacterial Seawater Whole Genome Sequencing Genome, Bacterial |
| url | https://pubmed.ncbi.nlm.nih.gov/41303395/ |