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| Main Authors: | , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
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International journal of systematic and evolutionary microbiology
2025
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| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41313628/ |
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| _version_ | 1868266119887847426 |
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| author | Rissanen, Antti J Hestnes, Anne Grethe Khanongnuch, Ramita Didriksen, Alena Urich, Tim Tveit, Alexander T Svenning, Mette M |
| author_facet | Rissanen, Antti J Hestnes, Anne Grethe Khanongnuch, Ramita Didriksen, Alena Urich, Tim Tveit, Alexander T Svenning, Mette M Rissanen, Antti J Hestnes, Anne Grethe Khanongnuch, Ramita Didriksen, Alena Urich, Tim Tveit, Alexander T Svenning, Mette M |
| collection | PubMed - marine biology |
| contents | sp. nov. isolated from a coal mine biofilm in the high Arctic Svalbard. Rissanen, Antti J Hestnes, Anne Grethe Khanongnuch, Ramita Didriksen, Alena Urich, Tim Tveit, Alexander T Svenning, Mette M RNA, Ribosomal, 16S Phylogeny DNA, Bacterial Base Composition Bacterial Typing Techniques Nucleic Acid Hybridization Svalbard Sequence Analysis, DNA Genome, Bacterial Biofilms Methane Coal Mining An aerobic methanotroph was isolated from a biofilm of coal mine Gruve 7 (Svalbard) and designated strain G7. Cells of strain G7 were Gram-stain-negative, pink-pigmented and motile rods. Strain G7 could grow at pH 6.8 and at temperatures ranging from 4 to 21 °C. The genome size was 4.00 Mb with a (digital) DNA G+C content of 47.7 mol%. Strain G7 represents a member of the family of the class . It displayed 94.6-99.7% 16S rRNA gene sequence similarity to the type strains of the genus . Whole-genome comparisons based on average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) confirmed that strain G7 represents a novel species. It showed 16S rRNA gene identity of 99.7%, 91.8% ANI and 46% dDDH to the closest type strain, LS7-T4A, with ANI and dDDH being much lower than the typically used 95 and 70% cutoffs, respectively, to delineate different species. For methane activation, strain G7 carries genes encoding particulate methane monooxygenase (pmoCAB). Also, genes of the methane utilization pathways, i.e. oxidation of methane to carbon dioxide and assimilation of methane-carbon to biomass, were encoded in the genome. Strikingly, compared to all other spp. strains, strain G7 did not have nitrogenase genes for nitrogen fixation. Strain G7 also possessed genes for ectoine production, which was not observed in the genomes of its closest relatives. Based on phenotypic, genetic and phylogenetic data, strain G7 represents a novel species within the genus for which the name sp. nov. is proposed, with strain G7 (DSM: 117899; LMG: 33632) as the type strain. |
| format | Artículo científico |
| id | pubmed_41313628 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | International journal of systematic and evolutionary microbiology |
| record_format | pubmed |
| spellingShingle | sp. nov. isolated from a coal mine biofilm in the high Arctic Svalbard. Rissanen, Antti J Hestnes, Anne Grethe Khanongnuch, Ramita Didriksen, Alena Urich, Tim Tveit, Alexander T Svenning, Mette M RNA, Ribosomal, 16S Phylogeny DNA, Bacterial Base Composition Bacterial Typing Techniques Nucleic Acid Hybridization Svalbard Sequence Analysis, DNA Genome, Bacterial Biofilms Methane Coal Mining sp. nov. isolated from a coal mine biofilm in the high Arctic Svalbard. Rissanen, Antti J Hestnes, Anne Grethe Khanongnuch, Ramita Didriksen, Alena Urich, Tim Tveit, Alexander T Svenning, Mette M RNA, Ribosomal, 16S Phylogeny DNA, Bacterial Base Composition Bacterial Typing Techniques Nucleic Acid Hybridization Svalbard Sequence Analysis, DNA Genome, Bacterial Biofilms Methane Coal Mining An aerobic methanotroph was isolated from a biofilm of coal mine Gruve 7 (Svalbard) and designated strain G7. Cells of strain G7 were Gram-stain-negative, pink-pigmented and motile rods. Strain G7 could grow at pH 6.8 and at temperatures ranging from 4 to 21 °C. The genome size was 4.00 Mb with a (digital) DNA G+C content of 47.7 mol%. Strain G7 represents a member of the family of the class . It displayed 94.6-99.7% 16S rRNA gene sequence similarity to the type strains of the genus . Whole-genome comparisons based on average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) confirmed that strain G7 represents a novel species. It showed 16S rRNA gene identity of 99.7%, 91.8% ANI and 46% dDDH to the closest type strain, LS7-T4A, with ANI and dDDH being much lower than the typically used 95 and 70% cutoffs, respectively, to delineate different species. For methane activation, strain G7 carries genes encoding particulate methane monooxygenase (pmoCAB). Also, genes of the methane utilization pathways, i.e. oxidation of methane to carbon dioxide and assimilation of methane-carbon to biomass, were encoded in the genome. Strikingly, compared to all other spp. strains, strain G7 did not have nitrogenase genes for nitrogen fixation. Strain G7 also possessed genes for ectoine production, which was not observed in the genomes of its closest relatives. Based on phenotypic, genetic and phylogenetic data, strain G7 represents a novel species within the genus for which the name sp. nov. is proposed, with strain G7 (DSM: 117899; LMG: 33632) as the type strain. |
| title | sp. nov. isolated from a coal mine biofilm in the high Arctic Svalbard. |
| topic | RNA, Ribosomal, 16S Phylogeny DNA, Bacterial Base Composition Bacterial Typing Techniques Nucleic Acid Hybridization Svalbard Sequence Analysis, DNA Genome, Bacterial Biofilms Methane Coal Mining |
| url | https://pubmed.ncbi.nlm.nih.gov/41313628/ |