Physicochemical characterization of bionanocomposite films based on tuna skin gelatin (Thunnus tonggol), squid ink melanin, and earthworm-derived glycolipoprotein G-90.
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| Format: | Artículo científico |
| Sprache: | en |
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International journal of biological macromolecules
2026
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| _version_ | 1868266119880507393 |
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| author | Elmi, Fatemeh Roshan, Mitra Esmaeilpour Elmi, Maryam Mitra Taleshi, Mojtaba Shokrollahzadeh |
| author_facet | Elmi, Fatemeh Roshan, Mitra Esmaeilpour Elmi, Maryam Mitra Taleshi, Mojtaba Shokrollahzadeh Elmi, Fatemeh Roshan, Mitra Esmaeilpour Elmi, Maryam Mitra Taleshi, Mojtaba Shokrollahzadeh |
| collection | PubMed - marine biology |
| contents | Physicochemical characterization of bionanocomposite films based on tuna skin gelatin (Thunnus tonggol), squid ink melanin, and earthworm-derived glycolipoprotein G-90. Elmi, Fatemeh Roshan, Mitra Esmaeilpour Elmi, Maryam Mitra Taleshi, Mojtaba Shokrollahzadeh Animals Gelatin Melanins Decapodiformes Skin Nanocomposites Oligochaeta Chemical Phenomena Wettability Ink Biocompatible Materials Tensile Strength Hydrophobic and Hydrophilic Interactions This study presents a sustainable strategy for developing multifunctional biopolymer-based composite films from marine and terrestrial bioresources. Gelatin extracted from Thunnus tonggol skin, melanin isolated from squid ink, and glycolipoprotein G-90 derived from earthworms were employed to enhance resource utilization within a circular bioeconomy framework. Four types of films namely gelatin, gelatin-melanin, gelatin-G-90, and gelatin-melanin-G-90 were fabricated and characterized for their structural, mechanical, physicochemical, and biological properties. ATR-FTIR spectra confirmed the successful incorporation of melanin and G-90, revealing strengthened hydrogen bonding and van der Waals interactions without altering gelatin's backbone conformation. Thermal analysis demonstrated that melanin improved stability by delaying degradation and enhancing water retention, while G-90 promoted synergistic stabilization in hybrid films. Contact angle measurements demonstrated tunable surface wettability, with melanin increasing hydrophobicity by 44 % (112.6 ± 9.1°), whereas G-90 introduced pronounced hydrophilicity by reducing WCA by ~57 % (33.8 ± 12.9°). Mechanical testing showed that melanin improved ductility and that G-90 alone reduced tensile strength but, when combined, produced a synergistic effect: the gelatin-melanin-G-90 film exhibited the highest elongation at break (101.6 ± 3.5 %, a ~ 200 % increase compared to pure gelatin) with moderate tensile strength (0.60 ± 0.1 MPa, ~51 % lower than gelatin). In vivo wound-healing evaluation revealed that the gelatin-melanin-G-90 film accelerated wound closure, enhanced angiogenesis, and supported nearly complete re-epithelialization. This work demonstrates a sustainable route for converting biological residues into thermally stable, mechanically flexible, and biologically active films with tunable wettability and strong wound healing performance. |
| format | Artículo científico |
| id | pubmed_41319763 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | International journal of biological macromolecules |
| record_format | pubmed |
| spellingShingle | Physicochemical characterization of bionanocomposite films based on tuna skin gelatin (Thunnus tonggol), squid ink melanin, and earthworm-derived glycolipoprotein G-90. Elmi, Fatemeh Roshan, Mitra Esmaeilpour Elmi, Maryam Mitra Taleshi, Mojtaba Shokrollahzadeh Animals Gelatin Melanins Decapodiformes Skin Nanocomposites Oligochaeta Chemical Phenomena Wettability Ink Biocompatible Materials Tensile Strength Hydrophobic and Hydrophilic Interactions Physicochemical characterization of bionanocomposite films based on tuna skin gelatin (Thunnus tonggol), squid ink melanin, and earthworm-derived glycolipoprotein G-90. Elmi, Fatemeh Roshan, Mitra Esmaeilpour Elmi, Maryam Mitra Taleshi, Mojtaba Shokrollahzadeh Animals Gelatin Melanins Decapodiformes Skin Nanocomposites Oligochaeta Chemical Phenomena Wettability Ink Biocompatible Materials Tensile Strength Hydrophobic and Hydrophilic Interactions This study presents a sustainable strategy for developing multifunctional biopolymer-based composite films from marine and terrestrial bioresources. Gelatin extracted from Thunnus tonggol skin, melanin isolated from squid ink, and glycolipoprotein G-90 derived from earthworms were employed to enhance resource utilization within a circular bioeconomy framework. Four types of films namely gelatin, gelatin-melanin, gelatin-G-90, and gelatin-melanin-G-90 were fabricated and characterized for their structural, mechanical, physicochemical, and biological properties. ATR-FTIR spectra confirmed the successful incorporation of melanin and G-90, revealing strengthened hydrogen bonding and van der Waals interactions without altering gelatin's backbone conformation. Thermal analysis demonstrated that melanin improved stability by delaying degradation and enhancing water retention, while G-90 promoted synergistic stabilization in hybrid films. Contact angle measurements demonstrated tunable surface wettability, with melanin increasing hydrophobicity by 44 % (112.6 ± 9.1°), whereas G-90 introduced pronounced hydrophilicity by reducing WCA by ~57 % (33.8 ± 12.9°). Mechanical testing showed that melanin improved ductility and that G-90 alone reduced tensile strength but, when combined, produced a synergistic effect: the gelatin-melanin-G-90 film exhibited the highest elongation at break (101.6 ± 3.5 %, a ~ 200 % increase compared to pure gelatin) with moderate tensile strength (0.60 ± 0.1 MPa, ~51 % lower than gelatin). In vivo wound-healing evaluation revealed that the gelatin-melanin-G-90 film accelerated wound closure, enhanced angiogenesis, and supported nearly complete re-epithelialization. This work demonstrates a sustainable route for converting biological residues into thermally stable, mechanically flexible, and biologically active films with tunable wettability and strong wound healing performance. |
| title | Physicochemical characterization of bionanocomposite films based on tuna skin gelatin (Thunnus tonggol), squid ink melanin, and earthworm-derived glycolipoprotein G-90. |
| topic | Animals Gelatin Melanins Decapodiformes Skin Nanocomposites Oligochaeta Chemical Phenomena Wettability Ink Biocompatible Materials Tensile Strength Hydrophobic and Hydrophilic Interactions |
| url | https://pubmed.ncbi.nlm.nih.gov/41319763/ |