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| Main Authors: | , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
BMC genomics
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41326993/ |
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Table of Contents:
- A mannitol-based buffer improves single-cell RNA sequencing of high-salt marine cells. Scully, Tal D Klein, Allon M Animals Single-Cell Analysis Mannitol Sequence Analysis, RNA Buffers Ciona intestinalis Salinity Gene Expression Profiling RNA-Seq Transcriptome Single-cell RNA sequencing (scRNA-seq) enables discovery of novel cell states by transcriptomic profiling with minimal prior knowledge, making it useful for studying non-model organisms. For most marine organisms, however, cells are viable at a higher salinity than is compatible with scRNA-seq, impacting data quality and cell representation. We show that a low-salinity phosphate buffer supplemented with D-mannitol (PBS-M) enables higher-quality scRNA-seq of blood cells from the tunicate Ciona robusta. Using PBS-M reduces cell death and ambient mRNA, revealing cell states not otherwise detected. We additionally validate PBS-M in a second tunicate species. This simple protocol modification could enable or improve scRNA-seq for many marine organisms.