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| Main Authors: | , , , , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Journal of agricultural and food chemistry
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41342874/ |
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Table of Contents:
- Chemobiocatalytic Production of 5-Hydroxymethyl-2-furancarboxylic Acid from Sucrose by a Choline Chloride-Lactic Acid Deep Eutectic Solvent and a Novel Strain. Qiu, Zhongyang Niu, Ruoyan Wu, Yue Wang, Binbin Deng, Xinzhu Shao, Wenjun Shao, Shuai Jin, Ci Deng, Yuanfang Xia, Jun Liu, Xiaoyan Xu, Jiaxing Sucrose Choline Lactic Acid Deep Eutectic Solvents Furans Furaldehyde Catalysis Biocatalysis Gluconobacter 5-Hydroxymethyl-2-furancarboxylic acid (HMFCA), a critical polyester intermediate, faces an industrial scale-up challenge due to the high production cost using commercial 5-hydroxymethylfurfural (HMF) as a substrate. This study developed an efficient chemobiocatalytic route to synthesize HMFCA from inexpensive sucrose. A newly isolated strain showed high HMF tolerance (246 mM) and completely converted HMF to HMFCA in 24 h with 99.3% yield (mol/mol). Meanwhile, the choline chloride-lactic acid deep eutectic solvent was found to efficiently convert sucrose to HMF, yielding 106.4 mM HMF (53.2% yield). The chemobiocatalytic potential of HMFCA production was evaluated using sucrose-derived crude HMF and whole-cell catalysis. Within 24 h, the crude HMF (64.8 mM) was completely consumed, and 63.5 mM HMFCA was produced. This integrated process achieved an overall HMFCA yield of approximately 52% based on the initial sucrose. These findings provide an efficient strategy for chemobiocatalytic conversion of inexpensive sucrose to high-value HMFCA.