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| Main Authors: | , , , , , , , , |
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| Format: | Artículo científico |
| Language: | en |
| Published: |
Organic letters
2025
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| Subjects: | |
| Online Access: | https://pubmed.ncbi.nlm.nih.gov/41347944/ |
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| _version_ | 1868266117029429249 |
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| author | Wu, Qiaoling Li, Feng Peng, Ming Chen, Yingying Weng, Xiang Xiao, Zhexiong Tian, Xinpeng Zhu, Hongjie Ju, Jianhua |
| author_facet | Wu, Qiaoling Li, Feng Peng, Ming Chen, Yingying Weng, Xiang Xiao, Zhexiong Tian, Xinpeng Zhu, Hongjie Ju, Jianhua Wu, Qiaoling Li, Feng Peng, Ming Chen, Yingying Weng, Xiang Xiao, Zhexiong Tian, Xinpeng Zhu, Hongjie Ju, Jianhua |
| collection | PubMed - marine biology |
| contents | Gene Cluster Directs Diversified Carbazole Biosynthesis via Cooperative Halogenation, Hydroxylation, and Methylation. Wu, Qiaoling Li, Feng Peng, Ming Chen, Yingying Weng, Xiang Xiao, Zhexiong Tian, Xinpeng Zhu, Hongjie Ju, Jianhua Carbazoles Multigene Family Methylation Molecular Structure Hydroxylation Halogenation Methyltransferases Chlocarbazomycins are bioactive carbazoles with therapeutic potential. Here, we report a conserved gene cluster () that directs its biosynthesis via a novel pathway. This cluster encodes a versatile tailoring system, including a regioselective halogenase (CczH), dedicated hydroxylases (CczA/J/D), and a bifunctional methyltransferase (CczI) that iteratively methylates and accelerates N-OH dehydroxylation. Our findings provide new enzymes for carbazole pathway engineering and structural diversification. |
| format | Artículo científico |
| id | pubmed_41347944 |
| institution | PubMed |
| language | en |
| publishDate | 2025 |
| publisher | Organic letters |
| record_format | pubmed |
| spellingShingle | Gene Cluster Directs Diversified Carbazole Biosynthesis via Cooperative Halogenation, Hydroxylation, and Methylation. Wu, Qiaoling Li, Feng Peng, Ming Chen, Yingying Weng, Xiang Xiao, Zhexiong Tian, Xinpeng Zhu, Hongjie Ju, Jianhua Carbazoles Multigene Family Methylation Molecular Structure Hydroxylation Halogenation Methyltransferases Gene Cluster Directs Diversified Carbazole Biosynthesis via Cooperative Halogenation, Hydroxylation, and Methylation. Wu, Qiaoling Li, Feng Peng, Ming Chen, Yingying Weng, Xiang Xiao, Zhexiong Tian, Xinpeng Zhu, Hongjie Ju, Jianhua Carbazoles Multigene Family Methylation Molecular Structure Hydroxylation Halogenation Methyltransferases Chlocarbazomycins are bioactive carbazoles with therapeutic potential. Here, we report a conserved gene cluster () that directs its biosynthesis via a novel pathway. This cluster encodes a versatile tailoring system, including a regioselective halogenase (CczH), dedicated hydroxylases (CczA/J/D), and a bifunctional methyltransferase (CczI) that iteratively methylates and accelerates N-OH dehydroxylation. Our findings provide new enzymes for carbazole pathway engineering and structural diversification. |
| title | Gene Cluster Directs Diversified Carbazole Biosynthesis via Cooperative Halogenation, Hydroxylation, and Methylation. |
| topic | Carbazoles Multigene Family Methylation Molecular Structure Hydroxylation Halogenation Methyltransferases |
| url | https://pubmed.ncbi.nlm.nih.gov/41347944/ |