Guardado en:
| Autores principales: | , , , , |
|---|---|
| Formato: | Artículo científico |
| Lenguaje: | en |
| Publicado: |
Microbiology spectrum
2026
|
| Materias: | |
| Acceso en línea: | https://pubmed.ncbi.nlm.nih.gov/41363855/ |
| Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
| _version_ | 1868266114441543681 |
|---|---|
| author | Akanbi, Taiwo Mercy Labat, Mariana Sun, Tyler Smith, Derek A Bagby, Sarah C |
| author_facet | Akanbi, Taiwo Mercy Labat, Mariana Sun, Tyler Smith, Derek A Bagby, Sarah C Akanbi, Taiwo Mercy Labat, Mariana Sun, Tyler Smith, Derek A Bagby, Sarah C |
| collection | PubMed - marine biology |
| contents | Isolation and characterization of TayeBlu, a novel bacteriophage of . Akanbi, Taiwo Mercy Labat, Mariana Sun, Tyler Smith, Derek A Bagby, Sarah C Azotobacter vinelandii Bacteriophages Soil Microbiology Rhizosphere Solanum lycopersicum Genome, Viral Nitrogen Fixation Phylogeny Nitrogen Soil microbial communities drive global biogeochemical cycles and alter crop yields through nitrogen fixation. As agents of genetic mobility, mortality, and nutrient release, viruses have been shown to influence microbial community structure and activity in numerous marine and aquatic systems. However, their impacts on terrestrial ecosystems are less well understood, in part because few model phage-host systems have been established for soils. To fill this gap, we sought to develop a new model system for viral infection of nitrogen-fixing bacteria derived from agricultural soil. Here, we report the isolation, characterization, and sequencing of the novel bacteriophage TayeBlu, which infects the globally distributed aerobic soil bacterium , a facultative diazotroph. TayeBlu was isolated from the rhizosphere of tomato plants in a farm greenhouse. We find that the availability of nitrogen to host cells strongly influences TayeBlu infection physiology at the level of adsorption kinetics, time to lysis, and burst size. Taxonomic and comparative genome analyses reveal that TayeBlu belongs to an understudied family in class in which a small core of structural and assembly genes has persisted through adaptive diversification on different bacterial hosts.IMPORTANCEUnderstanding the forces regulating soil microbial activity is critical for building accurate ecosystem models that can inform land-management strategies for mitigating climate risks and stabilizing global food supply. For agricultural sustainability, it is particularly important to understand the dynamics of nitrogen-fixing soil bacteria like , a well-studied and globally distributed species whose activity promotes plant growth and soil fertility. To support detailed investigations of the impact of viruses on diazotroph ecosystem outputs, we isolated and investigated a novel soil virus that infects . The resulting new phage-host system expands the limited toolkit of model experimental systems for soil viral studies. By enabling investigations of viral impacts on terrestrial nitrogen-fixing bacteria, this work sets the stage for future studies illuminating a critical but poorly understood aspect of soil ecology. Moreover, TayeBlu belongs to a novel viral family, and this study provides the first description of this family's conserved features. |
| format | Artículo científico |
| id | pubmed_41363855 |
| institution | PubMed |
| language | en |
| publishDate | 2026 |
| publisher | Microbiology spectrum |
| record_format | pubmed |
| spellingShingle | Isolation and characterization of TayeBlu, a novel bacteriophage of . Akanbi, Taiwo Mercy Labat, Mariana Sun, Tyler Smith, Derek A Bagby, Sarah C Azotobacter vinelandii Bacteriophages Soil Microbiology Rhizosphere Solanum lycopersicum Genome, Viral Nitrogen Fixation Phylogeny Nitrogen Isolation and characterization of TayeBlu, a novel bacteriophage of . Akanbi, Taiwo Mercy Labat, Mariana Sun, Tyler Smith, Derek A Bagby, Sarah C Azotobacter vinelandii Bacteriophages Soil Microbiology Rhizosphere Solanum lycopersicum Genome, Viral Nitrogen Fixation Phylogeny Nitrogen Soil microbial communities drive global biogeochemical cycles and alter crop yields through nitrogen fixation. As agents of genetic mobility, mortality, and nutrient release, viruses have been shown to influence microbial community structure and activity in numerous marine and aquatic systems. However, their impacts on terrestrial ecosystems are less well understood, in part because few model phage-host systems have been established for soils. To fill this gap, we sought to develop a new model system for viral infection of nitrogen-fixing bacteria derived from agricultural soil. Here, we report the isolation, characterization, and sequencing of the novel bacteriophage TayeBlu, which infects the globally distributed aerobic soil bacterium , a facultative diazotroph. TayeBlu was isolated from the rhizosphere of tomato plants in a farm greenhouse. We find that the availability of nitrogen to host cells strongly influences TayeBlu infection physiology at the level of adsorption kinetics, time to lysis, and burst size. Taxonomic and comparative genome analyses reveal that TayeBlu belongs to an understudied family in class in which a small core of structural and assembly genes has persisted through adaptive diversification on different bacterial hosts.IMPORTANCEUnderstanding the forces regulating soil microbial activity is critical for building accurate ecosystem models that can inform land-management strategies for mitigating climate risks and stabilizing global food supply. For agricultural sustainability, it is particularly important to understand the dynamics of nitrogen-fixing soil bacteria like , a well-studied and globally distributed species whose activity promotes plant growth and soil fertility. To support detailed investigations of the impact of viruses on diazotroph ecosystem outputs, we isolated and investigated a novel soil virus that infects . The resulting new phage-host system expands the limited toolkit of model experimental systems for soil viral studies. By enabling investigations of viral impacts on terrestrial nitrogen-fixing bacteria, this work sets the stage for future studies illuminating a critical but poorly understood aspect of soil ecology. Moreover, TayeBlu belongs to a novel viral family, and this study provides the first description of this family's conserved features. |
| title | Isolation and characterization of TayeBlu, a novel bacteriophage of . |
| topic | Azotobacter vinelandii Bacteriophages Soil Microbiology Rhizosphere Solanum lycopersicum Genome, Viral Nitrogen Fixation Phylogeny Nitrogen |
| url | https://pubmed.ncbi.nlm.nih.gov/41363855/ |